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Synthesis of hollow calcium carbonate nano-particles in a bubble column using carbonic anhydrase reaction in a bilamellar system

Research Project

Project/Area Number 25420833
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionYamaguchi University

Principal Investigator

Yoshimoto Makoto  山口大学, 医学(系)研究科(研究院), 准教授 (80322246)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords生体材料 / ナノ材料 / バイオリアクター / 酵素反応 / 生物・生体工学
Outline of Final Research Achievements

The carbonic anhydrase molecules were covalently conjugated to the negatively charged surface of phospholipid bilayer vesicles (liposomes). The liposomes were covered with different lipid bilayers to form the bilamellar liposomes. The formation of calcium carbonate was enhanced by the membrane-adsorbed calcium and the carbonic anhydrase-catalyzed hydration of carbon dioxide on the surface of liposomes with a carbon dioxide-saturated water solution. Furthermore, the process for the formation of calcium carbonate particles was developed by using the liposomal enzymes as catalysts in the gas-liquid contacting reactor (airlift bubble column) which was operated by continuously introducing carbon dioxide-containing gas.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (27 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 4 results) Presentation (19 results)

  • [Int'l Joint Research] スイス連邦工科大学(スイス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Enhanced heat stability of α-chymotrypsin through single-enzyme confinement in attoliter liposomes2016

    • Author(s)
      M. Yoshimoto, J. Yamada, M. Baba, P. Walde
    • Journal Title

      ChemBioChem

      Volume: 印刷中 Issue: 13 Pages: 1221-1224

    • DOI

      10.1002/cbic.201600150

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Liposomes as chaperone mimics with controllable affinity toward heat-denatured formate dehydrogenase from Candida boidinii2015

    • Author(s)
      Makoto Yoshimoto, Ryohei Kozono and Naoki Tsubomura
    • Journal Title

      Langmuir

      Volume: 31 Issue: 2 Pages: 762-770

    • DOI

      10.1021/la504126b

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Selective oxidation of D-amino acids catalyzed by oligolamellar liposomes intercalated with D-amino acid oxidase2014

    • Author(s)
      Makoto Yoshimoto, Masakazu Okamoto, Kouta Ujihashi and Takayuki Okita
    • Journal Title

      Langmuir

      Volume: 30 Issue: 21 Pages: 6180-6186

    • DOI

      10.1021/la500786m

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A kinetic analysis of catalytic production of oxygen in catalase-containing liposome dispersions for controlled transfer of oxygen in a bioreactor.2014

    • Author(s)
      Makoto Yoshimoto and Minato Higa
    • Journal Title

      J. Chem. Technol. Biotechnol.

      Volume: 89 Issue: 9 Pages: 1388-1395

    • DOI

      10.1002/jctb.4216

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mechanosensitive liposomes as artificial chaperones for shear-driven acceleration of enzyme-catalyzed reaction2014

    • Author(s)
      Tomotaka Natsume and Makoto Yoshimoto
    • Journal Title

      ACS Appl. Mater. Interfaces

      Volume: 6 Pages: 3671-3679

    • DOI

      10.1016/j.chemphyslip.2013.06.001

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phase transition-induced rapid permeabilization of liposome membranes composed of milk-sphingomyelin2014

    • Author(s)
      Makoto Yoshimoto and Yoshitaka Todaka
    • Journal Title

      Eur. J. Lipid Sci. Technol.

      Volume: 116 Issue: 2 Pages: 226-231

    • DOI

      10.1002/ejlt.201300332

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A method to estimate the average shear rate in a bubble column using liposomes2013

    • Author(s)
      Tomotaka Natsume and Makoto Yoshimoto
    • Journal Title

      Ind. Eng. Chem. Res.

      Volume: 52 Issue: 51 Pages: 18498-18502

    • DOI

      10.1021/ie402874q

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] リポソーム膜に結合させた炭酸脱水酵素と気泡塔による炭酸カルシウム粒子生成反応2016

    • Author(s)
      前島慶輔,相島賢司,吉本誠
    • Organizer
      混相流シンポジウム2016
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-08-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] イオン液体共存下のリポソーム複合化セルラーゼ活性に及ぼす脂質膜疎水性領域の影響2016

    • Author(s)
      三穂野海,大津豪志,大谷舞,吉本誠,上村明男
    • Organizer
      化学工学会第81年会
    • Place of Presentation
      関西大学(大阪府吹田市)
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 荷電脂質膜内のセルラーゼを用いたイオン液体共存下における不溶性セルロース糖化反応2015

    • Author(s)
      三穂野海,大津豪志,吉本誠,上村明男
    • Organizer
      化学工学会第47回秋季大会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] リン脂質ベシクルの微小液滴内に隔離された酵素一分子の特性と応用2015

    • Author(s)
      吉本誠,山田純
    • Organizer
      化学工学会第47回秋季大会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] セルラーゼ封入リポソームを用いたイオン液体共存下における不溶性セルロース糖化反応2014

    • Author(s)
      三穂野海,大津豪志,吉本誠,上村明男
    • Organizer
      化学工学会中国四国支部大学院生発表会
    • Place of Presentation
      RCC文化センター(広島市)
    • Year and Date
      2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 脂質膜の透過選択性と酵素安定化機能を利用するリポソーム系D-アミノ酸酸化反応2014

    • Author(s)
      吉本誠,岡本真和,宇治橋康太,沖田敬之
    • Organizer
      膜シンポジウム2014
    • Place of Presentation
      神戸大学(神戸市)
    • Year and Date
      2014-11-26 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Clusters of phospholipid vesicles as platforms for glucose oxidase-catalyzed reaction in a bubble column bioreactor2014

    • Author(s)
      Makoto Yoshimoto, Yuki Sakakida, Ryota Tamura, Tomotaka Natsume and Tomoaki Ikeda
    • Organizer
      2nd Int. Symp. Multiscale Multiphase Process Eng. (MMPE)
    • Place of Presentation
      Hamburg (Germany)
    • Year and Date
      2014-09-24 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Estimation of the shear rate in airlift and normal bubble columns on the basis of shear-dependent permeability of liposome membranes2014

    • Author(s)
      Tomotaka Natsume, Makoto Yoshimoto and Katsumi Nakao
    • Organizer
      2nd Int. Symp. Multiscale Multiphase Process Eng. (MMPE)
    • Place of Presentation
      Hamburg (Germany)
    • Year and Date
      2014-09-24 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] リポソームを用いる気泡塔内せん断速度に及ぼす装置形状と液深の影響の評価2014

    • Author(s)
      夏目友誉,吉本誠,中尾勝實
    • Organizer
      化学工学会第46回秋季大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 気液流動で分散性が変化するクラスター化リポソームを利用したグルコース酸化反応操作2014

    • Author(s)
      榊田祐希,吉本誠
    • Organizer
      化学工学会第46回秋季大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 蟻酸脱水素酵素の熱安定性に及ぼす共存脂質二分子膜の疎水鎖特性と電荷の影響2014

    • Author(s)
      小園遼平,吉本誠
    • Organizer
      化学工学会第46回秋季大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] リポソームを用いる微小円管内せん断流中のグルコース酸化酵素の活性化2014

    • Author(s)
      夏目友誉,吉本誠
    • Organizer
      化学工学会第79年会
    • Place of Presentation
      岐阜大学(岐阜市)
    • Related Report
      2013 Research-status Report
  • [Presentation] スフィンゴミエリン膜の特異な透過現象に基づく酵素反応制御2014

    • Author(s)
      吉本誠,戸高好貴
    • Organizer
      化学工学会第79年会
    • Place of Presentation
      岐阜大学(岐阜市)
    • Related Report
      2013 Research-status Report
  • [Presentation] リポソーム膜表層に共有結合させたD-アミノ酸酸化酵素の熱安定性2013

    • Author(s)
      岡本真和,吉本誠
    • Organizer
      化学工学会中国四国支部大会宇部大会
    • Place of Presentation
      山口大学(宇部市)
    • Related Report
      2013 Research-status Report
  • [Presentation] リポソームの可逆的クラスター化に及ぼす脂質膜組成の影響2013

    • Author(s)
      田村嶺多,夏目友誉,池田智昭,吉本誠
    • Organizer
      化学工学会中国四国支部大会宇部大会
    • Place of Presentation
      山口大学(宇部市)
    • Related Report
      2013 Research-status Report
  • [Presentation] カタラーゼ封入リポソーム懸濁系における過酸化水素分解反応の解析と酸素移動特性2013

    • Author(s)
      比嘉南斗,吉本誠
    • Organizer
      化学工学会第45回秋季大会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Research-status Report
  • [Presentation] スフィンゴミエリンベシクルの調製と膜透過性に及ぼす温度の影響2013

    • Author(s)
      戸高 好貴,吉本 誠
    • Organizer
      化学工学会第45回秋季大会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Research-status Report
  • [Presentation] カルシウムイオンによる混合脂質リポソームの可逆的クラスター化2013

    • Author(s)
      田村 嶺多,夏目 友誉,吉本 誠
    • Organizer
      化学工学会第45回秋季大会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Research-status Report
  • [Presentation] リポソーム膜の構造変化に基づく液体せん断ストレスセンシング2013

    • Author(s)
      夏目 友誉,吉本 誠
    • Organizer
      化学工学会第45回秋季大会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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