Egg-based oral immunotherapy using genetically manipulated chickens
| Project/Area Number |
25420834
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyushu University |
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Principal Investigator |
Kawabe Yoshinori 九州大学, 工学(系)研究科(研究院), 助教 (30448401)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 遺伝子導入ニワトリ / アレルゲンエピトープ / エピトープペプチド / スギ花粉症 / ワクチン卵 / M細胞 / 経口免疫ワクチン / MHC / トランスジェニックニワトリ卵 / アレルギー治療 / 経口免疫寛容 / RANKL / 花粉症モデルマウス / 腸管免疫寛容 / トランスジェニックニワトリ |
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| Outline of Final Research Achievements |
Here, we attempted to develop egg-based oral immunotherapy using genetically manipulated (GM) chickens. By using egg white derived from GM hens producing human T-cell epitopes fused with MHC molecules (MHC-Crp-Fc), the number of sneezing and IgE level were significantly decreased in allergic mice fed with MHC-Crp-Fc-containing egg white. TGF-beta1 could be produced in an oviduct-specific manner. Recombinant RANKL that can induce M cells was produced. Eggs derived from GM chickens may be served as edible pharmaceuticals for allergy immunotherapy.
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Report
(4 results)
Research Products
(11 results)
