Development of a molecular connection method for generating highly functional antibodies
Project/Area Number |
25420836
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
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Research Institution | Osaka City University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 抗体 / 酵素 / 受容体 / タンパク質工学 / 分子認識 |
Outline of Final Research Achievements |
In this study, we used a bacterial expression system to produce a bispecific antibody that was heterotetramerized through hetero-associating peptides and that targeted cancer cells. As the results, we succeeded in preparing the recombinant antibody which bound to two target molecules simultaneously. Furthermore, the bispecific antibody showed cytotoxic activity against target tumor cells, and its cytotoxicity was comparable to that of a commercial therapeutic antibody. These results show that our method has high potential for the cost-efficient production of highly functional antibodies.
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Report
(4 results)
Research Products
(19 results)
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[Presentation] 大腸菌発現系によるTNFα融合抗体の作製2015
Author(s)
大水 貴裕, 大﨑 智弘, 青木 基, 北村 昌也, 中西 猛
Organizer
BMB2015(第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会)
Place of Presentation
神戸国際展示場(兵庫県神戸市)
Year and Date
2015-12-01
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