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Development of high performance power-generation bio-devices using bio-finctional 3D-electrode

Research Project

Project/Area Number 25420837
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionSaitama Institute of Technology

Principal Investigator

Hasebe Yasushi  埼玉工業大学, 工学部, 教授 (20212144)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsバイオ電池 / バイオセンサ / 酵素 / タンパク質 / 酸化還元酵素 / 3次元電極 / 酵素機能電極 / 触媒電流 / バイオ発電デバイス / グルコースオキシダーゼ / グルコースデヒドロゲナーゼ / カーボンフェルト / 機能電極
Outline of Final Research Achievements

To develop highly functional protein-based bio-fuel cells, and self-powered biosensing devices, several redox-proteins and metal-proteins was successfully immobilized on the surface of carbon-felt, which is micro-electrode ensemble of micro carbon fiber (ca. 7 um in diameter) and possesses a random three-dimensional structure. To obtain larger bioelectrocatalytic current density, various experimental parameters (protein-immobilization method, kinds of buffer, pH, temperature and kinds of electron-transfer mediators) were optimized, and performance of these elecrodes were evaluated.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (20 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (7 results) Book (2 results) Remarks (3 results)

  • [Int'l Joint Research] 遼寧科技大学(中国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Carbon felt-based bioelectrocatalytic flow-through detectors: 2,6-dichlorophenol indophenol and peroxidase coadsorbed carbon-felt for flow-amperometric determination of hydrogen peroxide2014

    • Author(s)
      Yue Wang, Yasushi Hasebe
    • Journal Title

      Materials

      Volume: 7 Issue: 2 Pages: 1142-1154

    • DOI

      10.3390/ma7021142

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Poly(lactic acid) microparticles coated with insulin-containing LbL films and their pH-dependent insuline release2014

    • Author(s)
      R. Hashide, K. Yoshida, Y. Hasebe, M. Seno, S. Takahashi, K. Sato, and J. Anzai
    • Journal Title

      Journal of Nanoscience and Nanotechnology

      Volume: 14 Pages: 3100-3105

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Layer-by-layer deposited nano- and micro-assemblies for insuline delivery: A review2014

    • Author(s)
      K. Yoshida, Y. Hasebe, S. Takahashi, K. Sato, J. Anzai
    • Journal Title

      Mater. Sci. Eng. C

      Volume: 34 Pages: 384-392

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Layer-by-layer deposited nano- and micro-assemblies for insulin delivery: A review2014

    • Author(s)
      Kentaro Yoshida, Yasushi Hasebe, Shigehiro Takahashi, Katsuhiko Sato, Jun-ichi Anzai
    • Journal Title

      Materials Science and Engineering: C

      Volume: 34 Pages: 384-392

    • DOI

      10.1016/j.msec.2013.09.045

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hemin-adsorbed carbon felt for sensitive and rapid flow-amperometric detection of dissolved oxygen2013

    • Author(s)
      Yue Wang, Yasushi Hasebe
    • Journal Title

      Microchimica Acta

      Volume: 180 Issue: 13-14 Pages: 1295-1302

    • DOI

      10.1007/s00604-013-1057-9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sensitive voltammetric and amperometric responses of respiratory toxins at hemin-adsorbed carbon-felt2013

    • Author(s)
      Yasushi Hasebe, Yue Wang
    • Journal Title

      Journal of Environmental Sciences

      Volume: 25 Issue: 6 Pages: 1055-1062

    • DOI

      10.1016/s1001-0742(12)60128-4

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Protein and polysaccharide-composite sol-gel silicate film for an interference-free amperometric glucose biosensor2013

    • Author(s)
      Hironori Matsuhisa, Munenori Tsuchiya, Yasushi Hasebe
    • Journal Title

      Coloids and Surfaces B: Biointerfaces

      Volume: 111 Pages: 523-529

    • DOI

      10.1016/j.colsurfb.2013.06.046

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ウリカーゼ・ペルオキシダーゼ固定化カーボンフェルトを利用するFIA式尿酸センサ2016

    • Author(s)
      長谷部 靖、王 月、関 真一
    • Organizer
      第60回化学センサ研究発表会
    • Place of Presentation
      大阪大学
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 二種酵素固定化カーボンフェルトを利用するFIA式バイオセンサの開発2015

    • Author(s)
      渡辺 崇史、王 月、長谷部 靖
    • Organizer
      第59回化学センサ研究発表会
    • Place of Presentation
      埼玉工業大学
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Flow Amperometric Inhibition Type Biosensor Using Tyrosinase-Modified Carbon-Felt2014

    • Author(s)
      Yasushi Hasebe, Yue Wang, Yugo Kikuchi
    • Organizer
      19th International Conference on Flow Injection Analysis and Related Techniques
    • Place of Presentation
      ACROS Fukuoka
    • Year and Date
      2014-11-30 – 2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] チロシナーゼ固定化カーボンフェルトを用いるフロー型バイオセンサ2014

    • Author(s)
      奈良和幸、青木詩織、王月、長谷部靖
    • Organizer
      第57回化学センサ研究発表会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 分光学的手法によるグルコースオキシダーゼとメチレンブルーの結合相互作用解析2014

    • Author(s)
      青木詩織、王月、長谷部靖
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] センサシグナル増幅効果をもたらす有機色素とチロシナーゼの相互作用解析

    • Author(s)
      青木 詩織、王 月、長谷部 靖
    • Organizer
      第55回化学センサ研究発表会
    • Place of Presentation
      東京工業大学大岡山キャンパス(東京都目黒区大岡山2-12-1)
    • Related Report
      2013 Research-status Report
  • [Presentation] へミン吸着電極の電解触媒活性を利用する溶存酸素および呼吸毒の電気化学センシング

    • Author(s)
      細野 徹、王 月、長谷部 靖
    • Organizer
      第56回化学センサ研究発表会
    • Place of Presentation
      関西大学千里山キャンパス(吹田市山手町3-3-35)
    • Related Report
      2013 Research-status Report
  • [Book] 分子認識を基礎とする分析化学2015

    • Author(s)
      内山 俊一、長谷部 靖、浜名 浩、松浦 宏昭、斉藤 貴、吉田 泰彦、石井 茂、藤沼 弘
    • Total Pages
      207
    • Publisher
      宣協社
    • Related Report
      2015 Annual Research Report
  • [Book] 分子認識を基礎とする分析化学2013

    • Author(s)
      内山俊一、長谷部靖、浜名浩、松浦宏昭、斉藤貴、吉田泰彦、石井茂、藤沼弘
    • Total Pages
      207
    • Publisher
      専協社
    • Related Report
      2013 Research-status Report
  • [Remarks] 埼玉工業大学 長谷部研究室ホームページ

    • URL

      http://www.sit.ac.jp/user/hasebe/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 埼玉工業大学 バイオ機能デバイス(長谷部)研究室ホームページ

    • URL

      http://www.sit.ac.jp/user/hasebe/index.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 埼玉工業大学 工学部 生命環境化学科 長谷部研究室(バイオ機能デバイス研究室)

    • URL

      http://www.sit.ac.jp/user/hasebe/index.html

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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