Investigation of bio-volatilization in rare metal metabolizing bacterium
Project/Area Number |
25420838
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
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Research Institution | Shibaura Institute of Technology |
Principal Investigator |
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Research Collaborator |
OTSUKA Osamu
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | レアメタル / セレン / 生物気化 / メカニズム |
Outline of Final Research Achievements |
Pseudomonas stutzeri strain NT-I reduce selenate, selenite, and biogenic elemental selenium into volatilized selenium (Se), which was determined as dimethyldiselenide (DMDSe) by Gas Chromatograph Mass Spectrometer. The maximal culture conditions were 38℃, pH 9.0, agitation speed 250rpm, aeration rate 3L/min for DMDSe synthesis, the speed of which was 37.8μmol/h・L. The DMDSe synthesis rate is fastest among previous reports, demonstrating that strain NT-I is a promising biocalalyst for Se recovery. From the genome analysis in strain NT-I, the disA gene coding for DMDSe synthesis enzyme was cloned into a TA cloning vector. The recombinant Escherichia coli carrying disA gene produced DMDSe from selenite, and biogenic elemental selenium. Therefore, we discovered the disA as DMDSe synthesis gene.
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Report
(4 results)
Research Products
(28 results)
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[Presentation] Coprecipitation of radioactive strontium in sea water during formation of biogeneic calcite2015
Author(s)
T. Ohnuki, N. Kozai, F. Sakamoto, T. Saito, Q. Yu, M. Yamashita, T. Horiike, S. Utsunomiya.
Organizer
15th International Conference on the Chemistry and Migration Behaviour of Actinides and Fission Products in the Geosphere
Place of Presentation
Santa Fe Community Convention Center, Santa Fe, NM, USA
Year and Date
2015-09-13
Related Report
Int'l Joint Research
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