| Project/Area Number |
25420838
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Shibaura Institute of Technology |
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Principal Investigator |
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| Research Collaborator |
OTSUKA Osamu
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | レアメタル / セレン / 生物気化 / メカニズム |
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| Outline of Final Research Achievements |
Pseudomonas stutzeri strain NT-I reduce selenate, selenite, and biogenic elemental selenium into volatilized selenium (Se), which was determined as dimethyldiselenide (DMDSe) by Gas Chromatograph Mass Spectrometer. The maximal culture conditions were 38℃, pH 9.0, agitation speed 250rpm, aeration rate 3L/min for DMDSe synthesis, the speed of which was 37.8μmol/h・L. The DMDSe synthesis rate is fastest among previous reports, demonstrating that strain NT-I is a promising biocalalyst for Se recovery.
From the genome analysis in strain NT-I, the disA gene coding for DMDSe synthesis enzyme was cloned into a TA cloning vector. The recombinant Escherichia coli carrying disA gene produced DMDSe from selenite, and biogenic elemental selenium. Therefore, we discovered the disA as DMDSe synthesis gene.
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