Establish of a three-dimensional human peritoneal tissue evaluation method using the cell sheet lamination technology and its application to new treatment development

• Project/Area Number: 25420839
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Sakiyama Ryoichi 東京女子医科大学, 医学部, 助教 (30408471)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: solute permeability / peritoneal tissue / cell sheets / cell sheet lamination / kidney failure / 腹膜透析 / 積層化細胞シート / 溶質透過係数 / 腹膜組織モデル / 再生治療 / 組織工学 / 化学工学 / 再生医療

Outline of Final Research Achievements

This study is one of the development of techniques to assess whether PD liquid used in renal failure treatment is suitable for the living body. By stacking the cell sheet in vitro, it was successful in the production of pseudo-peritoneal tissue composed of three layers. Further, the pseudo-peritoneal tissue lumen structure of a single layer was observed vessels were observed. On the other hand, the solute permeability coefficient of the pseudo-peritoneal tissue, compared to a single-layer structure, 1 / 26.5 in molecular weight of 4000, 1 / 4.5 in molecular weight of 10,000, 1 / 1.9 in molecular weight of 70000, the solute is less likely to pass by organizing It becomes and it is shown that, became a result close to a living body.
To create a pseudo-peritoneal tissue in vitro, it has succeeded in the development of the evaluation method.

Research Products

• [Journal Article] Performance evaluation of developed polysulfone membrane hemodiafilters, ABH-F and ABH-P, in post- and pre-dilution hemodiafiltration. (2015)
  • Author(s): Sakiyama R, Yamamoto K, Ishimori I, Yoshida S, Akiba T, Mineshima M.
  • Journal Title: J Artif Organs Volume: 18 Issue: 4 Pages: 330-337
  • DOI: 10.1007/s10047-015-0844-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of core active disaccharides in heparin for HGF-inducing activity (2015)
  • Author(s): Takashi Kato, Ryoichi Sakiyama, Kiyomasa Oka, Toshikazu Nakamura
  • Journal Title: Journal of Pharmacology and Pharmacotherapeutics Volume: 6 Issue: 2 Pages: 77-82
  • DOI: 10.4103/0976-500x.155483
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] In vitro腹膜組織モデルを用いた溶質透過試験 (2016)
  • Author(s): 崎山亮一、大内奎、山下明泰、大和雅之、峰島 三千男
  • Organizer: 日本医工学治療学会第32回学術大会
  • Place of Presentation: 甲府富士屋ホテル
  • Year and Date: 2016-03-19
• [Presentation] 腹膜傷害評価の試みと3次元腹膜組織モデルの作製 (2016)
  • Author(s): 崎山亮一、大内奎、山下明泰、大和雅之、峰島 三千男
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17
• [Presentation] Performance Evaluation of a Super High Flux Dialyzer with Highly Enhanced Internal Filtration (2016)
  • Author(s): Sakiyama Ryoichi, Yamamoto Kenichiro, Ishimori Isamu, Okano Kazuhiro, Akiba Takashi, Mineshima Michio
  • Organizer: 36th Annual Dialysis Conference
  • Place of Presentation: The Washington State Convention Center
  • Year and Date: 2016-02-27
• [Presentation] 蛋白結合性尿毒素インドキシル硫酸の細胞への影響と細胞による解離特性 (2015)
  • Author(s): 崎山亮一、清水聖也、山下明泰、峰島 三千男
  • Organizer: 第53回日本人工臓器学会
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 2015-11-19
• [Presentation] on line 前希釈HDF条件下におけるABH-P溶質除去能の経時変化 (2015)
  • Author(s): 崎山亮一、山本健一郎、石森勇、岡野一祥、秋葉隆、峰島三千男
  • Organizer: 第21回日本HDF研究会学術集会・総会
  • Place of Presentation: ホテル日航熊本
  • Year and Date: 2015-09-26
• [Presentation] 腹膜モデルの作製と腹膜傷害因子による溶質透過亢進の評価 (2015)
  • Author(s): 崎山亮一、大内奎、山下明泰、峰島 三千男
  • Organizer: 化学工学会第47回秋季大会
  • Place of Presentation: 北海道大学工学部
  • Year and Date: 2015-09-09
• [Presentation] 細胞を用いた蛋白結合型尿毒素インドキシル硫酸の解離特性の基礎検討 (2015)
  • Author(s): 崎山亮一、清水聖也、山下明泰、峰島 三千男
  • Organizer: 第60回日本透析医学会学術集会．総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-06-25
• [Presentation] 腹膜モデルにおける溶質透過の検討 (2015)
  • Author(s): 大内 奎、﨑山 亮一、峰島 三千男、山下 明泰
  • Organizer: 化学工学会第80年会
  • Place of Presentation: 芝浦工業大学豊洲キャンパス
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] In vitro疑似腹膜モデル作製のための基礎検討 (2014)
  • Author(s): 大内 奎、﨑山 亮一、峰島 三千男、山下 明泰
  • Organizer: 化学工学会第46回秋季大会
  • Place of Presentation: 九州大学伊都キャンパス
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] Effect of Hepatocyte Growth Factor for Prevention of Solute Hyper Permeation in Mesothelial Cell Layer Induced by Methylglyoxal (2014)
  • Author(s): Sakiyama Ryoichi, Kanako Fukuda, Shimizu Mutumi, Sakai Kiyotaka, Mineshima Michio
  • Organizer: 34rd Annual Dialysis Conference
  • Place of Presentation: Atlanta, Gergia
• [Presentation] 中皮細胞層モデルを用いた腹膜傷害因子による溶質透過性亢進におけるHGFの抑制効果 (2014)
  • Author(s): 崎山 亮一、福田 佳那子、清水 睦、酒井 清孝、峰島 三千男
  • Organizer: 化学工学会第76回年会
  • Place of Presentation: 岐阜県岐阜市
• [Presentation] 中皮細胞層モデルを用いた溶質透過性亢進におけるHGFの抑制効果 (2014)
  • Author(s): 崎山 亮一、福田 佳那子、清水 睦、酒井 清孝、峰島 三千男
  • Organizer: 日本医工学治療学会 第30回学術大会
  • Place of Presentation: 愛知県名古屋市