Development of novel glycan probe for monitoring molecular chaperone activity.
| Project/Area Number |
25420844
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | University of Toyama (2014-2015)
Institute of Physical and Chemical Research (2013) |
|---|
Principal Investigator |
SAKONO MASAFUMI 富山大学, 大学院理工学研究部(工学), 准教授 (50391959)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 分子シャペロン / レクチン / 糖鎖 / 小胞体シャペロン / 糖タンパク質品質管理 / 分子間相互作用 / 環境応答性分子 |
|---|
| Outline of Final Research Achievements |
NileRed derivatives combined with spacer were synthesized. These derivatives were connected to amidated glycan, resulting that glycan which has environmental responsible ability. Recombinant human CRT was produced by E. coli. A cDNA encoding human CRT was cloned into pCold I expression plasmid , which was designed to produce N-terminally (His)6-tagged proteins.
|
Report
(4 results)
Research Products
(6 results)
