Development of novel glycan probe for monitoring molecular chaperone activity.

• Project/Area Number: 25420844
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: University of Toyama (2014-2015); Institute of Physical and Chemical Research (2013)
• Principal Investigator: SAKONO MASAFUMI 富山大学, 大学院理工学研究部(工学), 准教授 (50391959)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 分子シャペロン / レクチン / 糖鎖 / 小胞体シャペロン / 糖タンパク質品質管理 / 分子間相互作用 / 環境応答性分子

Outline of Final Research Achievements

NileRed derivatives combined with spacer were synthesized. These derivatives were connected to amidated glycan, resulting that glycan which has environmental responsible ability. Recombinant human CRT was produced by E. coli. A cDNA encoding human CRT was cloned into pCold I expression plasmid , which was designed to produce N-terminally (His)6-tagged proteins.

Research Products

• [Journal Article] Hydrophobic Tagged Dihydrofolate Reductase for Creating Misfolded Glycoprotein Mimetics (2016)
  • Author(s): Masakazu Hachisu, Akira Seko, Shusaku Daikoku, Yoichi Takeda, Masafumi Sakono, Yukishige Ito
  • Journal Title: ChemBioChem Volume: 17 Issue: 4 Pages: 300-303
  • DOI: 10.1002/cbic.201500595
  • Peer Reviewed
• [Journal Article] PDI family protein ERp29 forms 1:1 complex with lectin chaperone calreticulin. (2014)
  • Author(s): Masafumi Sakono, Akira Seko, Yoichi Takeda, Yukishige Ito
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 452 Issue: 1 Pages: 27-31
  • DOI: 10.1016/j.bbrc.2014.08.041
  • Peer Reviewed
• [Journal Article] Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions. (2014)
  • Author(s): Masafumi Sakono, Akira Seko, Yoichi Takeda, Jun-ichi Aikawa, Masakazu Hachisu, Akihiko Koizumi, Kohki Fujikawa, Yukishige Ito
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1840 Issue: 9 Pages: 2904-2913
  • DOI: 10.1016/j.bbagen.2014.04.012
  • Peer Reviewed
• [Journal Article] Parallel quantification of lectin-glycan interaction using ultrafiltration (2013)
  • Author(s): Yoichi Takeda, Akira Seko, Masafumi Sakono, Masakazu Hachisu, Akihiko Koizumi, Kohki Fujikawa, Yukishige Ito
  • Journal Title: Carbohydrate Research Volume: 375 Pages: 112-117
  • DOI: 10.1016/j.carres.2013.04.032
  • Peer Reviewed
• [Journal Article] Folding of Synthetic Homogeneous Glycoproteins in the Presence of a Glycoprotein Folding Sensor Enzyme (2013)
  • Author(s): Simone Dedola, Masayuki Izumi, Yutaka Makimura, Akira Seko, Akiko Kanamori, Masafumi Sakono, Yukishige Ito, Yasuhiro Kajihara
  • Journal Title: Angewandte Chemie International Edition Volume: 53 Issue: 11 Pages: 2883-2887
  • DOI: 10.1002/anie.201309665
  • Peer Reviewed
• [Presentation] 合成糖鎖によるレクチン様分子シャペロンの基質認識機構の解明 (2016)
  • Author(s): 迫野昌文
  • Organizer: 第22回ペプチドフォーラム
  • Place of Presentation: 金沢大学サテライト・プラザ
  • Year and Date: 2016-03-05
  • Invited