| Project/Area Number |
25430012
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IKEDA KEIKO 兵庫医科大, 医学部, 教授 (10265241)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Phox2b / 呼吸中枢 / 延髄 / トランスジェニックラット / 光遺伝学 / archrhodopsin / EYFP / pFRG / 呼吸リズム / カルシウムイメージング / オプトジェネティクス / 中枢化学受容器 |
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| Outline of Final Research Achievements |
We have analyzed neuron networks of the medullary respiratory center using transgenic (Tg) rats in which fluorescent protein (EYFP) or photosensitive ion channels, archaerhodopsin (Arch) were expressed under control of Phox2b gene expression. First, we confirmed specific expression of EYFP in Phox2b positive cells of Tg rats. The central respiratory activity in the brainstem-spinal cord preparation from Tg rats did not differ from that of wild type rats. Using calcium imagings, we confirmed that many of EYFP positive cells in the pFRG showed Pre-I neuron like activity. We also confirmed that optogenetics is useful for analysis of respiratory rhythm generation in the en bloc newborn rat preparation from Arch expressing Tg rats.
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