In vivo whole-cell patch-clamp analysis of correlation between ECoG and the membrane potentials of cortical neurons in awake rats.
| Project/Area Number |
25430013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Hagoromo International University (2015)
Tamagawa University (2013-2014) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISOMURA Yoshikazu 玉川大学, 脳科学研究所, 教授 (00415077)
FUJIYAMA Fumino 同志社大学, 脳科学研究科, 教授 (20244022)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脳波 / 大脳皮質 / 錐体細胞 / 線条体 / 中型有棘神経細胞 / インビボ / パッチクランプ / 無麻酔 |
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| Outline of Final Research Achievements |
It is said that electrocorticogram (ECoG) reflects the summation of the synchronous activity of post synaptic potentials occured in cortical neurons. However, the generation mehcanisms of ECoG isn't elucidated in detail yet. Since the movement of animals causes the failure of stable whole-cell patch-clamp recordings, the comparison of the fluctuations of membrane potentials with ECoG without anesthesia has been difficult. In this study, we succeeded in stable simultaneous recordings of ECoG and the membrane potentials of pyramidal cells in cortical M1 area or of medium spiny neurons in striatum in awake, task-performing rats. We observed high correlation between membrane potential fluctuations and the wave form of ECoG, and its behavior-related changes were apparent.
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Report
(4 results)
Research Products
(7 results)
