A role of Plexin A2/A4 signaling in layer formation of the mouse cerebral cortex
| Project/Area Number |
25430020
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
HATANAKA Yumiko 生理学研究所, 基盤神経科学研究領域, 特別協力研究員 (40271548)
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| Co-Investigator(Kenkyū-buntansha) |
平田 たつみ 国立遺伝学研究所, 総合遺伝研究系, 教授 (80260587)
川崎 能彦 国立遺伝学研究所, 総合遺伝研究系, 助教 (00322751)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 神経発生 / 細胞移動 / 層形成 / 大脳皮質形成 / 細胞間相互作用 / 解剖学 / 発生・分化 / 脳・神経 / 神経細胞移動 / 発生•分化 |
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| Outline of Final Research Achievements |
We investigated a phenotype of Plexin A2/A4 double mutant mouse, in which a fraction of upper layer neurons of the cerebral cortex failed to terminate migration and ectopically invaded layer 1. PlexinA2/A4 proteins were expressed on leading processes of upper layer neurons and rescue experiment indicated that Plexin A2/A4 played a cell-autonomous role. On the other hand, Semaphorin 6A, the ligand for Plexin A2/A4, was concentrated in layer 1 and found to originate from radial glial cells in the ventricular zone. These results provide a novel model for termination of neuronal migration; (1) neurons migrate along radial glial cell fiber to reach the top of the cortical plate, (2) Plexin A2/A4-positive leading process touch the distal part of radial glial cell fiber in layer 1, which expresses Semaphorin 6A, and (3) probably due to repulsive effect, interaction between these molecules leads to detachment of migrating neurons from their substrate, thereby terminating migration.
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Report
(5 results)
Research Products
(8 results)
