Elucidation of Neural Circuit Mechanisms Underlying Transformation of Olfactory Inputs into Behavioral Outputs

• Project/Area Number: 25430025
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Miyasaka Nobuhiko 国立研究開発法人理化学研究所, 脳科学総合研究センター, 副チームリーダー (70332335)
• Research Collaborator: YOSHIHARA Yoshihiro ; KOIDE Tetsuya ; WAKISAKA Noriko ; MASUDA Miwa ; YABUKI Yoichi
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 嗅覚 / 神経回路 / 脳 / 行動 / 遺伝子工学 / 視床下部 / 嗅覚受容体 / ゼブラフィッシュ / 嗅球 / 間脳

Outline of Final Research Achievements

Odor information is represented as a chemotopic map in the olfactory bulb. This odor map must be transferred to higher brain centers and translated into appropriate behavioral responses. In the present study, we analyzed neural circuit mechanisms underlying transformation of odor inputs into behavioral outputs in zebrafish. We identified an olfactory receptor for prostaglandin F2α (PGF2α), a female sex pheromone, and found that male zebrafish mutant for this receptor show impaired courtship behaviors toward female fish. Olfactory stimulation with PGF2α activated a ventral telencephalic nucleus and several hypothalamic nuclei. ATP and alanine, food-derived odors, attracted zebrafish and commonly activated several brain regions including the posterior tuberculum, which constitutes the olfactory bulb-hypothalamus direct pathway. Furthermore, we identified brain regions that are specifically activated by different odor stimuli.

Research Products

• [Journal Article] An adenosine receptor for olfaction in fish (2017)
  • Author(s): Wakisaka N., Miyasaka N., Koide T., Masuda M., Hiraki-Kajiyama T., Yoshihara Y.
  • Journal Title: Current Biology Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Olfactory receptor for prostaglandin F2α mediates male fish courtship behavior. (2016)
  • Author(s): Yabuki Y, Koide T, Miyasaka N, Wakisaka N, Masuda M, Ohkura M, Nakai J, Tsuge K, Tsuchiya S, Sugimoto Y, Yoshihara Y
  • Journal Title: Nature Neuroscience Volume: 19 Issue: 7 Pages: 897-904
  • DOI: 10.1038/nn.4314
  • Peer Reviewed / Open Access
• [Journal Article] Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling (2014)
  • Author(s): Nobuhiko Miyasaka, Ignacio Arganda-Carreras, Noriko Wakisaka, Miwa Masuda, Uygar Sumbul, H. Sebastian Seung, Yoshihiro Yoshihara
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 3639-3639
  • DOI: 10.1038/ncomms4639
  • Peer Reviewed
• [Presentation] Olfactory receptor for prostaglandin F2α mediates courtship behavior of male zebrafish (2016)
  • Author(s): Yabuki Y., Koide T., Miyasaka N., Wakisaka N, Masuda M., Ohkura M., Nakai J., Tsuge K., Tsuchiya S., Sugimoto Y., Yoshihara Y.
  • Organizer: 17th International Symposium on Olfaction and Taste (ISOT)
  • Place of Presentation: パシフィコ横浜（神奈川・横浜）
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labeling
  • Author(s): Nobuhiko Miyasaka
  • Organizer: Workshop on Sensory Systems
  • Place of Presentation: 東京工業大学 横浜市
  • Invited
• [Presentation] Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labeling
  • Author(s): Nobuhiko Miyasaka, Yoshihiro Yoshihara
  • Organizer: The 2nd "International Institute for Advanced Studies" Conference of Novel Developments of Life and Biological Systems Based on Genome Engineering and Imaging Science
  • Place of Presentation: 国際高等研究所 京都府
  • Invited
• [Book] Immunocytochemistry and Related Techniques (Neuromethods Vol. 101), Chapter 4: Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain. (2015)
  • Author(s): Nobuhiko Miyasaka, Noriko Wakisaka, Yoshihiro Yoshihara
  • Total Pages: 12
  • Publisher: Springer
• [Remarks] 嗅覚神経回路の精緻な配線図の解読に成功
  • URL: http://www.riken.jp/pr/press/2014/20140409_1/