| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
CREB-dependent gene expression is critical for long-term memory (LTM) formation. In Drosophila single CREB (dCREB2) gene generates both activator dCREB2a and 2d and repressor dCREB2b isoforms by alternative splicing. Thus, it has been suggested that activator to repressor (activator/repressor) ratio is critical for LTM-related gene expression and this ratio is increased by spaced training which produces LTM. However, the splicing mechanism that regulates activator/repressor ratio is not yet known.
To explore the mechanism that regulates activator/repressor ratio, we focused on K-homology(KH) domain proteins which regulates alternative splicing of mRNA. In this study we report a KH domain protein which expression was increased by overexpressing dCREB2d, during spaced training. We suspect that this KH domain protein may play critical role in activator/repressor regulation during LTM formation.
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