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Developmental process of cerebellar compartmentalization and topographic axonal projections as basis for cerebellar functional localization

Research Project

Project/Area Number 25430032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SUGIHARA Izumi  東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60187656)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsマウス / 小脳 / 縦縞状区画構造 / Pcdh10 / レポーターマウス / 形成過程 / プルキンエ細胞 / プロトカドヘリン10 / 軸索投射 / アルドラーゼC / 神経解剖学 / 下オリーブ核 / 遺伝子改変マウス / 軸索再構築
Outline of Final Research Achievements

We analyzed the process of formation of the cerebellar compartmentalization and topographic axonal projection, which are important for varieties of cerebellar functions such as the control of many motor activities. Seven compartments elongated in the rostrocaudal direction were recognized in the early cerebellum at the embryonic day 14.5 (E14.5). The process in which these compartments were separated and divided into some 50 compartments in three days by E17.5 was clarified in the three-dimensional space. It was also observed that topographic axonal connection was formed first in the cerebellar nuclei, which then leads to compartmentalization. Results obtained in the related research projects were published in five research papers in international academic journals with the principal investigator as the corresponding author during the three-year period of the grant support. However, some central results are still under preparation for publication.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (19 results) (of which Invited: 5 results) Remarks (2 results)

  • [Journal Article] Compartmentalization of the chick cerebellar cortex based on the link between the striped expression pattern of aldolase C and the topographic olivocerebellar projection2015

    • Author(s)
      Vibulyaseck S, Luo Y, Fujita H, Oh-Nishi A, Ohki-Hamazaki H, Sugihara
    • Journal Title

      J Comp Neurol

      Volume: 印刷中 Issue: 13 Pages: 1886-1912

    • DOI

      10.1002/cne.23769

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 小脳の解剖(小葉構造)と基本的な機能局在2015

    • Author(s)
      杉原泉
    • Journal Title

      医学のあゆみ

      Volume: 255 Pages: 927-933

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Detailed expression pattern of aldolase C (aldoc) in the cerebellum, retina and other areas of the CNS studied in aldoc-venus knock-in mice.2014

    • Author(s)
      Fujita H, Aoki H, Ajioka I, Yamazaki M, Abe M, Oh-Nishi A, Sakimura K, Sugihara I.
    • Journal Title

      PLoS One.

      Volume: 9(1) Issue: 1 Pages: e86679-e86679

    • DOI

      10.1371/journal.pone.0086679

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Systematic analysis of neuronal wiring of the rodent deep cerebellar nuclei reveals differences reflecting adaptations at the neuronal circuit and internuclear level2014

    • Author(s)
      Hamodeh S, Baizer J, Sugihara I, Sultan F
    • Journal Title

      J Comp Neurol

      Volume: 522(11) Issue: 11 Pages: 2481-2497

    • DOI

      10.1002/cne.23545

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cerebellar afferents originating from the medullary reticular formation that are different from mossy, climbing or monoaminergic fibers in the rat2014

    • Author(s)
      Luo Y, Sugihara I
    • Journal Title

      Brain Res

      Volume: 1566 Pages: 31-46

    • DOI

      10.1016/j.brainres.2014.04.020

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Systematic regional variations in Purkinje cell spiking patterns.2014

    • Author(s)
      Xiao J, Cerminara NL, Kotsurovskyy Y, Aoki H, Burroughs A, Wise AK, Luo Y, Marshall SP, Sugihara I, Apps R, Lang EJ
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 8 Pages: e105633-e105633

    • DOI

      10.1371/journal.pone.0105633

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Modulation of Purkinje cell complex spike waveform by synchrony levels in the olivocerebellar system.2014

    • Author(s)
      Lang EJ, Tang T, Suh CY, Xiao J, Kotsurovskyy Y, Blenkinsop TA, Marshall SP, Sugihara I
    • Journal Title

      Front Syst Neurosci

      Volume: 8 Pages: 210-210

    • DOI

      10.3389/fnsys.2014.00210

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Morphology of the olivocerebellar projection of the chick: An axonal reconstruction study.2013

    • Author(s)
      Sasanuma, K., Ohki-Hamazaki, H. and Sugihara, I.
    • Journal Title

      J. Comp. Neurol.

      Volume: 521 Issue: 14 Pages: 3321-3339

    • DOI

      10.1002/cne.23352

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural basis of cerebellar microcircuits in the rat2013

    • Author(s)
      Cerminara N, Aoki H, Loft M, Sugihara I, Apps R
    • Journal Title

      J Neurosci.

      Volume: 33(42) Issue: 42 Pages: 16427-16442

    • DOI

      10.1523/jneurosci.0861-13.2013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 小葉の立体構造、軸索投射、分子発現パタンの解析に基づくヒト、ニホンザル、マーモセット、ラット、マウスの小脳半球部小葉構造の相同性2016

    • Author(s)
      杉原 泉
    • Organizer
      第121回日本解剖学会
    • Place of Presentation
      ビッグパレットふくしま, 郡山
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Optogenetic dissection of multiple signals in single cerebellar granules cells in vivo.2016

    • Author(s)
      Misa Shimuta, Izumi Sugihara, Taro Ishikawa
    • Organizer
      The 93th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      札幌コンベンションセンター, 札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Single axon morphology of vestibulocerebellar mossy fibers indicates the phylogenetically old nature of the vestibulocerebellum2015

    • Author(s)
      Izumi Sugihara, Mitsuhito Ueda, Takahiro Ando, Yuanjun Luo
    • Organizer
      The 38th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Kobe Convention Center, Kobe
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Reconstruction of spinocerebellar single axons in the mouse2015

    • Author(s)
      YuanJun Luo, Izumi Sugihara
    • Organizer
      The 38th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Kobe Convention Center, Kobe
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Tail proprioceptice representation area in the cerebellum identified by using Aldoc-venus knock-in mice.2015

    • Author(s)
      Takeru Onozato, Yuanjun Luo, Izumi Sugihara
    • Organizer
      The 92th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      神戸国際会議場・展示場、兵庫県神戸市
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Reconstruction of BDA-labeled long projecting axons in the central nervous system in bright-field serial section specimen2015

    • Author(s)
      Yuanjun Luo, Izumi Sugihara
    • Organizer
      CBIR Young Investigator Symposium
    • Place of Presentation
      東京医科歯科大学、東京都文京区
    • Year and Date
      2015-02-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 小脳における縦縞区画可視化Aldoc-Vinusマウスを用いた in vivo 実験について2015

    • Author(s)
      Takeru Onozato, Kenji Sakimura, Izumi Sugihara
    • Organizer
      第7回CBIR・ONSA 若手インスパイアシンポジウム
    • Place of Presentation
      東京医科歯科大学、東京都文京区
    • Year and Date
      2015-02-21
    • Related Report
      2014 Research-status Report
  • [Presentation] アルドラーゼC発現の縞構造と登上線維投射の部位対応性に基づいたトリ小脳の区画構造2014

    • Author(s)
      Yuanjun Luo, Suteera Vibulyaseck, Hirofumi Fujita, Hiroko Ohki-Hamazaki, and Izumi Sugihara
    • Organizer
      平成26年度『包括型脳科学研究推進支援ネットワーク』冬のシンポジウム
    • Place of Presentation
      東京医科歯科大学、ホテル東京ガーデンパレス、東京都文京区
    • Year and Date
      2014-12-11 – 2014-12-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Aldoc-Venusマウスによる小脳縦縞区画可視化とin vivo実験への応用2014

    • Author(s)
      小野里尊、崎村建司、杉原泉
    • Organizer
      平成26年度『包括型脳科学研究推進支援ネットワーク』冬のシンポジウム
    • Place of Presentation
      東京医科歯科大学、ホテル東京ガーデンパレス、東京都文京区
    • Year and Date
      2014-12-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Protocadherin 10 expression pattern in the developing mouse cerebellar cortex.2014

    • Author(s)
      VIBULYASECK; S. HIRANO; I. SUGIHARA
    • Organizer
      Neuroscience 2014  (北米神経科学会大会)
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 感覚運動適応能力の基盤としての小脳と前庭系の神経回路2014

    • Author(s)
      杉原 泉
    • Organizer
      第42回秋田めまい懇話会
    • Place of Presentation
      秋田メトロポリタンホテル、秋田県秋田市
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Striped Protocadherin 10 expression pattern in the adult and developing mouse cerebellar cortex2014

    • Author(s)
      Suteera Vibulyaseck, Shinji Hirano, Izumi Sugihara
    • Organizer
      The 37th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Pacifico Yokohama, Kanagawa, Yokohama
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Projection patterns of individual spinocerebellar axons in the mouse.2014

    • Author(s)
      Yuanjun Luo, Kazuma Sasamura, Radhika Pooja Patel, Izumi Sugihara
    • Organizer
      The 37th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Pacifico Yokohama, Kanagawa, Yokohama
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of functional compartmentalization of the cerebellum2014

    • Author(s)
      Suteera Vibulyaseck, Shinji Hirano, Izumi Sugihara
    • Organizer
      Physiology Symposium 2014 & Joint Scientific Conference of The Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong
    • Place of Presentation
      Hong Kong, Hong Kong Special Administrative Region of the People's Republic of China
    • Year and Date
      2014-06-12 – 2014-06-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular compartments and axonal projections in the cerebellum.2014

    • Author(s)
      Sugihara, I
    • Organizer
      A course in Molecular Neuroanatomy MNA 2014
    • Place of Presentation
      Okinawa
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Compartmentalization of the cerebellar nuclei and cortex based on input and output axonal projections and molecular expressions2013

    • Author(s)
      Sugihara, I
    • Organizer
      GRC on the Cerebellum
    • Place of Presentation
      New London, NH, U.S.A.
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Basic organization of the mammalian cerebellum as revealed by detailed analysis of aldolase C compartmentalization in the marmoset.2013

    • Author(s)
      Sugihara, I
    • Organizer
      6th Igakuken International Symposium Marmoset Neuroscience
    • Place of Presentation
      Tokyo
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 小脳の構造と機能

    • Author(s)
      杉原泉
    • Organizer
      小脳研究会
    • Place of Presentation
      東京、都市センターホテル
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Longitudinal stripes and the climbing fiber projection in the avian cerebellum

    • Author(s)
      Suteera Vibulyaseck, Kazuma Sasamura, Hiroko Ohki-Hamazaki, Izumi Sugihara
    • Organizer
      CBIR若手インスパイアシンポジウム
    • Place of Presentation
      Tokyo
    • Related Report
      2013 Research-status Report
  • [Remarks] システム神経生理学分野

    • URL

      http://www.tmd.ac.jp/med/phy1/phy1.html

    • Related Report
      2013 Research-status Report
  • [Remarks] Systems Neurophysiology

    • URL

      http://www.tmd.ac.jp/med/eng/eng/phy1-E.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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