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Targeted disruption of a gene that encodes a signaling molecule for a neurite branching.

Research Project

Project/Area Number 25430033
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionNational Institute for Physiological Sciences (2015-2016)
Niigata University (2013-2014)

Principal Investigator

Kishi Masashi  生理学研究所, 生体機能調節研究領域, 特別協力研究員 (60573938)

Research Collaborator FURUSE Mikio  生理学研究所, 生体機能調節研究領域, 教授
NAGAOKA Tadahiro  生理学研究所, 生体機能調節研究領域, 特別協力研究員
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords神経突起 / 蛋白質リン酸化 / 行動解析 / シナプス / ミトコンドリア / 行動テストバッテリー / 酵母two-hybrid法 / エネルギー産生 / 蛋白凝集 / 神経突起の内径 / 抗ニューロフィラメント抗体 / 結合蛋白
Outline of Final Research Achievements

A protein kinase called BrancK expressed in the mouse brain was analyzed in vivo. Targeted disruption of BrancK in mice revealed that this molecule is important for normal formation and branching of the neurites of the neurons of the central and peripheral nervous systems. We further analyzed morphological, behavioral, and biochemical defects of the mutant mice, and identified a disease phenotype and binding proteins. These results shed light on the role for a protein kinase signaling in the development of human brain and psychiatric diseases.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 2013

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] The planar cell polarity protein Vangl2 is involved in postsynaptic compartmentalization.2016

    • Author(s)
      Nagaoka T, Kishi M.
    • Journal Title

      Neurosci Lett.

      Volume: 612 Pages: 251-255

    • DOI

      10.1016/j.neulet.2015.12.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] PDZ interaction of Vangl2 links PSD-95 and Prickle2 but plays only a limited role in the synaptic localisation of Vangl2.2015

    • Author(s)
      Nagaoka T, Tabuchi K, Kishi M.
    • Journal Title

      Sci Rep.

      Volume: 5 Issue: 1 Pages: 12916-12916

    • DOI

      10.1038/srep12916

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Vangl2 regulates E-cadherin in epithelial cells.2014

    • Author(s)
      Nagaoka, T., Inutsuka, A., Begum, K., Bin hafiz, K. & Kishi, M.
    • Journal Title

      Scientific reports

      Volume: 4 Issue: 1 Pages: 6940-6940

    • DOI

      10.1038/srep06940

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Simple and Highly Efficient Method to Identify the Integration Site of a Transgene in the Animal Genome.2014

    • Author(s)
      Uemura S, Nagaoka T, Yokoyama M, Igarashi M, Kishi M.
    • Journal Title

      Neurosci. Res.

      Volume: 80 Pages: 91-94

    • DOI

      10.1016/j.neures.2013.11.007

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] SAD kinases control the maturation of nerve terminals in the mammalian peripheral and central nervous systems.2014

    • Author(s)
      Lilley B, Krishnaswamy A, Wang Z, Kishi M, Frank E, Sanes JR.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111 Issue: 3 Pages: 1138-1143

    • DOI

      10.1073/pnas.1321990111

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Wnt/Planar Cell Polarity Pathway Component Vangl2 Induces Synapse Formation through Direct Control of N-Cadherin.2014

    • Author(s)
      Nagaoka T, Ohashi R, Inutsuka A, Sakai S, Fujisawa N, Yokoyama M, Huang YH, Igarashi M, Kishi M
    • Journal Title

      Cell Rep

      Volume: 6 Issue: 5 Pages: 916-27

    • DOI

      10.1016/j.celrep.2014.01.044

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Role of planar cell polarity protein Vangl2 in synapse formation.2015

    • Author(s)
      T. Nagaoka, M. Kishi
    • Organizer
      2015 cell biology ascb annual meeting.
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Planar Cell Polarity in the Neural Circuit.2015

    • Author(s)
      Masashi Kishi, Tadahiro Nagaoka
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
  • [Presentation] Planar Cell Polarity in Synapse Formation2015

    • Author(s)
      Masashi Kishi, Tadahiro Nagaoka
    • Organizer
      Neuro2015
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Roles of Cell Polarity Regulators at Mammalian Synapsses.2013

    • Author(s)
      Kishi M, Nagaoka T, Uemura S., Inutsuka A., Sakai S., Fujisawa N., Yokoyama M., Igarashi M.
    • Organizer
      Neuro2013 (The 36th annual Meeting of the Japan Neuroscience Society)
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Identification of S1 antigen which interacts with and enhances the internalization of N-cadherin.2013

    • Author(s)
      Kishi M, Nagaoka T, Uemura S., Ohashi R., Inutsuka A., Sakai S., Fujisawa N., Yokoyama M., Igarashi M.
    • Organizer
      XXIII International Symposium on Morphological Sciences (symposium organization: Neuron and Circuit Formation)
    • Place of Presentation
      Niigata, Japan
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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