Investigation of mechanisms of dendritic differentiation of cerebellar Purkinje cells mediated by ryanodine receptors
| Project/Area Number |
25430040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Nagoya City University |
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Principal Investigator |
Tanaka Masahiko 名古屋市立大学, 薬学研究科(研究院), 准教授 (60267953)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | リアノジン受容体 / カルシウム / プルキンエ細胞 / 樹状突起 / 小脳 / siRNA / 単一細胞エレクトロポレーション / CaM kinase / 顆粒細胞 |
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| Outline of Final Research Achievements |
We investigated mechanisms of dendritic differentiation of cerebellar Purkinje cells mediated by ryanodine receptors (RyRs). Using single-cell electroporation of siRNA and calcium imaging, we revealed that RyR type 1 expressed by Purkinje cells promotes dendritic differentiation of Purkinje cells through phosphorylation of the common substrates of calcium/calmodulin-dependent protein kinase (CaMK) IIa, IIb and IV. On the other hand, RyR type 2 expressed by granule cells was also found to promote dendritic differentiation of Purkinje cells through secretion of brain-derived neurotrophic factor (BDNF) from granule cells.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] ZIP2 protein, a zinc transporter, is associated with keratinocyte differentiation2014
Author(s)
Yu Inoue, Seiji Hasegawa, Sadanori Ban, Takaaki Yamada, Yasushi Date, Hiroshi Mizutani, Satoru Nakata, Masahiko Tanaka, Naohide Hirashima
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Journal Title
Journal of Biological Chemistry
Volume: 289
Issue: 31
Pages: 21451-21462
DOI
Related Report
Peer Reviewed
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