| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We investigated mechanisms of dendritic differentiation of cerebellar Purkinje cells mediated by ryanodine receptors (RyRs). Using single-cell electroporation of siRNA and calcium imaging, we revealed that RyR type 1 expressed by Purkinje cells promotes dendritic differentiation of Purkinje cells through phosphorylation of the common substrates of calcium/calmodulin-dependent protein kinase (CaMK) IIa, IIb and IV. On the other hand, RyR type 2 expressed by granule cells was also found to promote dendritic differentiation of Purkinje cells through secretion of brain-derived neurotrophic factor (BDNF) from granule cells.
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