Study of the central olfactory projection with genetic methods

• Project/Area Number: 25430045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: National Institute of Genetics
• Principal Investigator: KAWASAKI Takahiko 国立遺伝学研究所, 総合遺伝研究系, 助教 (00322751)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経回路形成 / 軸索ガイダンス

Outline of Final Research Achievements

Conditional KO mice for target axon guidance signaling were made. With proper activity of the Cre recombinase, these mice showed abnormal phenotype in the axon guidance. However, two mice lines which express Cre recombinase in the olfactory bulb could not show enough activity to induce abnormal network formation in the central olfactory system. The tTA/tetO system also did not work well in the central olfactory projection.

Research Products

• [Journal Article] The COUP-TFII/Neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain (2015)
  • Author(s): Shigeaki Kanatani, Takao Honda, Michihiko Aramaki, Kanehiro Hayashi, Ken-ichiro Kubo, Mami Ishida, Daisuke H. Tanaka, Takeshi Kawauchi, Katsutoshi Sekine, Sayaka Kusuzawa, Takahiko Kawasaki, Tatsumi Hirata, Hidenori Tabata, Per Uhlen and Kazunori Nakajima
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 112(36) Issue: 36
  • DOI: 10.1073/pnas.1420701112
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Semaphorin 4D induces vaginal epithelial cell apoptosis to control mouse postnatal vaginal tissue remodeling. (2015)
  • Author(s): Ito T, Bai T, Tanaka T; Yoshida K, Ueyama T, Miyajima M, Negishi T, Kawasaki T, Takamatsu H, Kikutani H, Atsushi Kumanogoh A, Yukawa K
  • Journal Title: Mol Med Rep Volume: 11 Issue: 2 Pages: 829-836
  • DOI: 10.3892/mmr.2014.2773
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcallosal projections require glycoprotein M6-dependent neurite growth and guidance. (2014)
  • Author(s): S. Mita, P. Monasterio-Schrader, U. Fünfschilling, T. Kawasaki, H. Mizuno, T. Iwasato, K.-A. Nave, H. B. Werner, and T. Hirata
  • Journal Title: Cerebral Cortex Volume: [Epub ahead of print] Issue: 11 Pages: 4111-4125
  • DOI: 10.1093/cercor/bhu129
  • Peer Reviewed
• [Journal Article] Estrogen-dependent proteolytic cleavage of semaphorin 4D and plexin-B1 enhances semaphorin 4D-induced apoptosis during postnatal vaginal remodeling in pubescent mice. (2014)
  • Author(s): Ito T, Bai T, Tanaka T, Yoshida K, Ueyama T, Miyajima M, Negishi T, Kawasaki T, Takamatsu H, Kikutani H, Kumanogoh A, Yukawa K
  • Journal Title: PLoS One Volume: 9
  • Peer Reviewed / Open Access