Tumor-associated macrophages and microvascular proliferation in glioblastoma
| Project/Area Number |
25430051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOKOO Hideaki 群馬大学, 医学(系)研究科(研究院), 教授 (40282389)
本間 琢 日本大学, 医学部, 助教 (00307852)
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| Co-Investigator(Renkei-kenkyūsha) |
HONMA Taku 日本大学, 医学部, 助教 (00307852)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ミクログリア / マクロファージ / グリオーマ / 腫瘍随伴マクロファージ / 血管新生 / 微小血管増殖 / M1マクロファージ / M2マクロファージ / Ｍ１マクロファージ / 脳腫瘍 |
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| Outline of Final Research Achievements |
We examined human glioblastoma (GBs) to obtain a detailed alteration of tumor-associated macrophages (TAMs) within and around proliferating vessels. Expression of various macrophage markers, including Iba1, CD68, CD163, CD204, Glut5, HLA-DR, was evaluated in surgically resected GBs using immunohistochemistry as well as double immunofluorescence. 3D images were reconstructed in glomeruloid vascular proliferation with Iba1 immunohistocemistry using recent computer imaging software. Iba1-positive, TAMs increased around the MVP structure. The expression of CD204 was most frequently shown, followed by that of CD163, but HLA-DR was rarely found. Double immunofluorescence showed the different localization of Iba1 and a-SMA in TAMs and pericytic cells, respectively. 3D image analysis showed the accumulation of Iba1-labeled macrophages along the vascular wall of glomeruloid vessel, These results indicate that M2-like TAMs might be an important molecule for TAMs contributing to MVP in GB.
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Report
(4 results)
Research Products
(9 results)
