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Tumor-associated macrophages and microvascular proliferation in glioblastoma

Research Project

Project/Area Number 25430051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionSaitama Medical University

Principal Investigator

Sasaki Atsushi  埼玉医科大学, 医学部, 教授 (80225862)

Co-Investigator(Kenkyū-buntansha) YOKOO Hideaki  群馬大学, 医学(系)研究科(研究院), 教授 (40282389)
本間 琢  日本大学, 医学部, 助教 (00307852)
Co-Investigator(Renkei-kenkyūsha) HONMA Taku  日本大学, 医学部, 助教 (00307852)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsミクログリア / マクロファージ / グリオーマ / 腫瘍随伴マクロファージ / 血管新生 / 微小血管増殖 / M1マクロファージ / M2マクロファージ / M1マクロファージ / 脳腫瘍
Outline of Final Research Achievements

We examined human glioblastoma (GBs) to obtain a detailed alteration of tumor-associated macrophages (TAMs) within and around proliferating vessels. Expression of various macrophage markers, including Iba1, CD68, CD163, CD204, Glut5, HLA-DR, was evaluated in surgically resected GBs using immunohistochemistry as well as double immunofluorescence. 3D images were reconstructed in glomeruloid vascular proliferation with Iba1 immunohistocemistry using recent computer imaging software. Iba1-positive, TAMs increased around the MVP structure. The expression of CD204 was most frequently shown, followed by that of CD163, but HLA-DR was rarely found. Double immunofluorescence showed the different localization of Iba1 and a-SMA in TAMs and pericytic cells, respectively. 3D image analysis showed the accumulation of Iba1-labeled macrophages along the vascular wall of glomeruloid vessel, These results indicate that M2-like TAMs might be an important molecule for TAMs contributing to MVP in GB.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014 2013 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results) Presentation (5 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Variable expression of microglial DAP12 and TREM2 genes in Nasu-Hakola disease.2015

    • Author(s)
      Sasaki A, Kakita A, Yoshida K, Konno T, Ikeuchi T, Hayashi S, Matsuo H, Shioda K
    • Journal Title

      Neurogenetics

      Volume: 16 Issue: 4 Pages: 265-276

    • DOI

      10.1007/s10048-015-0451-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Intratumoral heterogeneity of genomic imbalance in a case of epithelioid glioblastoma with BRAF V600E mutation.2014

    • Author(s)
      Nobusawa S, Hirato J, Kurihara H, Ogawa A, Okura N, Nagaishi M, Ikota H, Yokoo H, Nakazato Y.
    • Journal Title

      Brain Pathol

      Volume: 24 Issue: 3 Pages: 239-246

    • DOI

      10.1111/bpa.12114

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of microglia/macrophages in gliomas developed in S100β-v-erbB transgenic rats2013

    • Author(s)
      Sasaki A, Yokoo H, Nakazato Y
    • Journal Title

      Neuropathology

      Volume: - Issue: 5 Pages: 505-514

    • DOI

      10.1111/neup.12015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Tumor-associated macrophages and microvascular proliferation in glioblastoma2016

    • Author(s)
      Atsushi Sasaki
    • Organizer
      21st International Conference on Brain Tumor Research and Therapy
    • Place of Presentation
      万国津梁館(沖縄県名護市)
    • Year and Date
      2016-04-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 悪性グリオーマ組織内微小血管増殖における腫瘍随伴マクロファージの特性について2015

    • Author(s)
      佐々木 惇
    • Organizer
      第56回日本神経病理学会総会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膠芽腫(GBM)患者長期生存例の検討:多施設共同研究.2014

    • Author(s)
      藤巻高光,脇谷健司,佐々木惇
    • Organizer
      第32回日本脳腫瘍学会
    • Place of Presentation
      シェラトン・グランデ・トーキョーベイ・ホテル(千葉県浦安市)
    • Year and Date
      2014-12-01
    • Related Report
      2014 Research-status Report
  • [Presentation] 乏突起膠腫成分を伴う膠芽腫の細胞像.2014

    • Author(s)
      金野美年子,本間琢,政岡秀彦,瀬山幸子,土居美枝子,望野唯明,清水道生,佐々木惇
    • Organizer
      第53回日本臨床細胞学会秋期大会
    • Place of Presentation
      下関市民会館(山口県下関市)
    • Year and Date
      2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Clinicopathologic features of pineal parenchymal tumors in a single institutional study.2014

    • Author(s)
      Sasaki A, Ishizawa K, Shimizu M, Nishikawa R, Honma T
    • Organizer
      International Congress of Neuropathology
    • Place of Presentation
      Sheraton Rio Hotel(Rio de Janeiro, Brasil)
    • Year and Date
      2014-09-15
    • Related Report
      2014 Research-status Report
  • [Remarks] 埼玉医科大学病理学・中央病理診断部

    • URL

      http://www.saitama-pathology.com/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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