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Analyses of class II ARF family proteins

Research Project

Project/Area Number 25430061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionGunma University

Principal Investigator

Sadakata Tetsushi  群馬大学, テニュアトラック普及推進室, 講師 (90391961)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsTremor / 振戦 / ARF / trafficking / tremor
Outline of Final Research Achievements

ADP-ribosylation factors (ARFs) constitute a family of small monomeric GTPases. The ARF family consists of three classes and a total of six members: class I, consisting of ARFs 1-3; class II, consisting of ARFs 4 and 5; and class III, which comprises ARF6. Little is known about the role of the class II ARFs in membrane trafficking. Here, we generated class II ARF knockout mice, and found that ARF4+/-/ARF5-/- mice showed severe tremor. Propranolol and gabapentin, which alleviate symptoms in essential tremor patients, reduced the amplitude of the pathologic tremor. Immunochemical studies showed that Nav1.6 proteins, normally localized in axon initial segment (AIS), was highly decreased in ARF4+/-/ARF5-/- Purkinje cell. These results indicate that the impairment of electrophysiological properties of Purkinje cell by Nav1.6 decrease is related to the onset of pathologic tremor of essential tremor patients.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 4 results,  Open Access: 4 results) Presentation (6 results) (of which Invited: 5 results)

  • [Journal Article] Interaction of Ca2+-dependent activator protein for secretion 1 (CAPS1) with septin family proteins in mouse brain2016

    • Author(s)
      Hosono M, Shinoda Y, Hirano T, Ishizaki Y, Furuichi T and Sadakata T
    • Journal Title

      Neurosci. Lett.

      Volume: 617 Pages: 232-235

    • DOI

      10.1016/j.neulet.2016.02.035

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of gene expression in Ca2+-dependent activator protein for secretion 2 (Cadps2) knockout cerebellum using GeneChip and KEGG pathways2016

    • Author(s)
      Sadakata T, Shinoda Y, Ishizaki Y, Furuichi T.
    • Journal Title

      Neuroscience Letters

      Volume: 639 Pages: 88-93

    • DOI

      10.1016/j.neulet.2016.12.068

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 自閉症関連遺伝子CAPS2の解析2016

    • Author(s)
      定方哲史
    • Journal Title

      生化学

      Volume: 88 Pages: 501-505

    • NAID

      40020948554

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Lack of stress responses to long-term effects of corticosterone in Caps2 knockout mice.2015

    • Author(s)
      Yuriko Mishima, Yo Shinoda, Tetsushi Sadakata, Masami Kojima, Shigeharu Wakana, Teiichi Furuichi.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 1-6

    • DOI

      10.1038/srep08932

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Age-dependent redistribution and hypersialylation of the central myelin paranodal loop membrane protein Opalin in the mouse brain.2014

    • Author(s)
      Yumi Sato, Fumio Yoshikawa, Tetsushi Sadakata, Yo Shinoda, Michinori Koebis, Teiichi Furuichi.
    • Journal Title

      Neurosci Letter

      Volume: 581 Pages: 14-19

    • DOI

      10.1016/j.neulet.2014.08.017

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Axonal localization of Ca2+-dependent activator protein for secretion 2 is critical for subcellular locality of brain-derived neurotrophic factor and neurotrophin-3 release affecting proper development of postnatal mouse cerebellum.2014

    • Author(s)
      Sadakata T, Kakegawa W, Shinoda Y, Hosono M, Katoh-Semba R, Sekine Y, Sato Y, Saruta C, Ishizaki Y, Yuzaki M, Kojima M, Furuichi T.
    • Journal Title

      PLoS One.

      Volume: 9(6) Issue: 6 Pages: 1-10

    • DOI

      10.1371/journal.pone.0099524

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mouse models of mutations and variations in autism spectrum disorder-associated genes : mice expressing caps2/cadps2 copy number and alternative splicing variants2013

    • Author(s)
      Sadakata T, Shinoda Y, Sato A, Iguchi H, Ishii C, Matsuo M, Yamaga R, Furuichi T
    • Journal Title

      Int J Environ Res Public Health

      Volume: 10 Issue: 12 Pages: 6335-6353

    • DOI

      10.3390/ijerph10126335

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] CAPS1 deficiency perturbs dense-core vesicle trafficking and Golgi structure and reduces presynaptic release probability in the mouse brain.2013

    • Author(s)
      Sadakata T, Kakegawa W, Shinoda Y, Hosono M, Katoh-Semba R, Sekine Y, Sato Y, Tanaka M, Iwasato T, Itohara S, Furuyama K, Kawaguchi Y, Ishizaki Y, Yuzaki M, Furuichi T.
    • Journal Title

      J Neurosci.

      Volume: 33 Issue: 44 Pages: 17326-17334

    • DOI

      10.1523/jneurosci.2777-13.2013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Animal models of autism spectrum disorder (ASD): a synaptic-level approach to autistic-like behavior in mice.2013

    • Author(s)
      Shinoda Y, Sadakata T, Furuichi T.
    • Journal Title

      Exp Anim.

      Volume: 62(2) Pages: 71-8

    • NAID

      10031163545

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Autistic-like behavioral phenotypes in a mouse model with copy number variation of the CAPS2/CADPS2 gene2013

    • Author(s)
      Sadakata T, Shinoda Y, Oka M, Sekine Y, Furuichi T.
    • Journal Title

      FEBS Lett

      Volume: 587 Issue: 1 Pages: 54-59

    • DOI

      10.1016/j.febslet.2012.10.047

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] CAPS2のexon3スキップと自閉症2015

    • Author(s)
      定方哲史
    • Organizer
      BMB2015(第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸ポートアイランド(兵庫、神戸)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 自閉症モデルマウスを用いた包括的解析2015

    • Author(s)
      定方哲史
    • Organizer
      第2回生物学的自閉症研究会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2015-07-19
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 有芯小胞分泌不全と自閉症2014

    • Author(s)
      定方哲史
    • Organizer
      第36回日本生物学的精神医学会・第57回日本神経化学会大会合同年会
    • Place of Presentation
      奈良
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 自閉症関連遺伝子CAPS22014

    • Author(s)
      定方哲史
    • Organizer
      第61回北関東医学会総会
    • Place of Presentation
      前橋
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Reduced axonal localization of a Caps2 splice variant impairs axonal release of BDNF and causes autistic-like behavior in mice2013

    • Author(s)
      Tetsushi Sadakata, Yo Shinoda, Megumi Oka, Yukiko Sekine, Yumi Sato, Chihiro Saruta, Hideki Miwa, Mika Tanaka, Shigeyoshi Itohara, and Teiichi Furuichi.
    • Organizer
      43th Annual Meeting of Society for Neuroscience
    • Place of Presentation
      San Diego, California, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 自閉症モデルマウスを用いた発症メカニズムの解明2013

    • Author(s)
      定方哲史
    • Organizer
      平成25年度生理学研究所研究会
    • Place of Presentation
      岡崎
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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