| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In the present study, we attempted to establish a novel method to monitor chaperone-mediated autophagy (CMA) and microautophagy (mA) acitivities, separately. siRNA-mediated knockdown of CMA- and mA-related proteins enabled us to assess CMA and mA activities, separately, in cells expressing GAPDH-HT, a reporter protein of CMA/mA activity. Using this method, we revealed that the CMA/mA activity is significantly decreased in drug-induced cell model of Pakinson's disease. Moreover, a chemical that activates CMA prominently inhibited cell death in this model. These findings strongly suggest that distrubance of CMA/mA activity would render the pathogenesis of Parkinson's disease.
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