• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The formation of GABAergic network and rhyhm of repiratory in the embryonic development

Research Project

Project/Area Number 25430066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionUniversity of the Ryukyus

Principal Investigator

SHIMIZU Chigusa  琉球大学, 医学(系)研究科(研究院), 特命助教 (70435072)

Co-Investigator(Kenkyū-buntansha) OKABE Akihito  琉球大学, 大学院医学研究科, 准教授 (10313941)
Co-Investigator(Renkei-kenkyūsha) TAKAYAMA Chitoshi  琉球大学, 大学院医学研究科, 教授 (60197217)
YANAGAWA Yuchio  群馬大学, 大学院医学研究科, 教授 (90202366)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
KeywordsGABA / KCC2 / hypogrossal / VGAT / GAD / 呼吸リズム / 舌下神経核 / 細胞内Cl-濃度 / 延髄
Outline of Final Research Achievements

GABA plays important roles in the respiratory rhythm. Role of GABAergic transmission in regulation of medullary respiration-related rhythmic activity (RRA) perinatally, however is yet to be determined. In this study the numbers of RRA were significantly increased in embryonic development but there were no significant changes after birth. VGAT transports GABA to synaptic vesicles. GABAergic action is changed from excitatory to inhibitory by expression of KCC2. Both VGAT and KCC2 deficient mice die in their birthday by respiratory failure. By compared these mice, it found that exocytosis of GABA is necessary to generate RRA, however KCC2 needs for making of rhythm of respiratory. .

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (39 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (28 results) Remarks (3 results)

  • [Journal Article] A novel insulinotropic mechanism of whole grain-derived γ-oryzanol via the suppression of local dopamine D2 receptor signalling in mouse islet.2015

    • Author(s)
      Kozuka C, Sunagawa S, Ueda R, Higa M, Ohshiro Y, Tanaka H, Shimizu-Okabe C, Takayama C, Matsushita M, Tsutsui M, Ishiuchi S, Nakata M, Yada T, Miyazaki JI, Oyadomari S, Shimabukuro M, Masuzaki H.
    • Journal Title

      Br J Pharmacol

      Volume: 172 Issue: 18 Pages: 4519-4534

    • DOI

      10.1111/bph.13236

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] γ-oryzanol protects pancreatic β-cells against endoplasmic reticulum stress in male mice.2015

    • Author(s)
      Kozuka C, Sunagawa S, Ueda R, Higa M, Tanaka H, Shimizu-Okabe C, Ishiuchi S, Takayama C, Matsushita M, Tsutsui M, Miyazaki JI, Oyadomari S, Shimabukuro M, Masuzaki H
    • Journal Title

      Endocrinology

      Volume: 156 Issue: 4 Pages: 1242-1250

    • DOI

      10.1210/en.2014-1748

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] KCC2-mediated regulation of respiration-related rhythmic activity during postnatal development in mouse medulla oblongata.2015

    • Author(s)
      Okabe A., Shimizu-Okabe C., Arata A., Konishi S., Fukuda A., and Takayama C.
    • Journal Title

      Brain Res.

      Volume: 1601 Pages: 31-39

    • DOI

      10.1016/j.brainres.2015.01.007

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of a novel cell-penetrating peptide targeting human glioblastoma cell lines as a cancer-homing transporter.2015

    • Author(s)
      Higa M, Katagiri C, Shimizu-Okabe C, Tsumuraya T, Sunagawa M, Nakamura M, Ishiuchi S, Takayama C, Kondo E, Matsushita M.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 457(2) Issue: 2 Pages: 206-12

    • DOI

      10.1016/j.bbrc.2014.12.089

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of neuropsin in parvalbumin immunoreactivity changes in hippocampal basket terminals of mice reared in various environments2014

    • Author(s)
      Suzuki H, Kanagawa D, Nakazawa H, Tawara-Hirata Y, Kogure Y, Shimizu-Okabe C, Takayama C, Ishikawa Y, Shiosaka S
    • Journal Title

      Front Cell Neurosci

      Volume: 8 Pages: 420-420

    • DOI

      10.3389/fncel.2014.00420

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Embryonic development of GABAergic signaling in the mouse spinal trigeminal nucleus interpolaris2014

    • Author(s)
      Hidemichi Kin, Jeongtae Kim, Chigusa Shimizu-Okabe, Akihito Okabe, Chitoshi Takayama
    • Journal Title

      Neuroscience Letters

      Volume: 566 Pages: 221-225

    • DOI

      10.1016/j.neulet.2014.02.057

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characteristic development of the GABA-removal system in the mouse spinal cord.2014

    • Author(s)
      Kim J, Kosaka Y, Shimizu-Okabe C, Niizaki A, Takayama C.
    • Journal Title

      Neuroscience.

      Volume: 262 Pages: 129-142

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 子持ち研究者, 日本解剖学会男女共同参画ワークショップに参加する2013

    • Author(s)
      清水千草
    • Journal Title

      解剖学雑誌

      Volume: 88 Pages: 67-68

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス坐骨神経損傷モデルにおけるGABA伝達関連分子の発現変化2016

    • Author(s)
      小坂祥範、屋富祖司、金正泰、清水千草、高山千利
    • Organizer
      第121回日本解剖学会
    • Place of Presentation
      ビッグパレットふくしま(福島県福島市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] wx/ae brown rice reduced the fat accumulation and improved dyslipidemia.2016

    • Author(s)
      Chigusa Shimizu,Chisayo Kozuka, Shiori Kobayashi, Yu Miyazaki, Jeongtae Kim, Hiroaki Masuzaki, Chitoshi Takayama.
    • Organizer
      第93回日本生理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス坐骨神経損傷モデルにおけるGABA伝達関連分子の発現変化2016

    • Author(s)
      小坂祥範、屋富祖司、金正泰、清水千草、高山千利
    • Organizer
      第93回日本生理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Development of inhibitory system in the mouse spinal cord2016

    • Author(s)
      清水千草
    • Organizer
      第93回日本生理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Sequential embryonic development of GABAergic input the hypothalamus2015

    • Author(s)
      Shimizu-Okabe C., Kobayashi M., Kim J., Niizaki A., Matsushita M., Masuzaki H., and Takayama C.
    • Organizer
      第38回日本神経科学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28 – 2015-07-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of glycinergic system in the mouse spinal cord.2015

    • Author(s)
      Sunagawa M., Shimizu-Okabe C., Kim J., Kobayashi S., and Takayama C.
    • Organizer
      第38回日本神経科学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28 – 2015-07-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Sequential embryonic development of GABAergic input the hypothalamus2015

    • Author(s)
      Shimizu-Okabe C., Kobayashi M., Kim J., Niizaki A., Matsushita M., Masuzaki H., and Takayama C.
    • Organizer
      第38回日本神経科学会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Development of glycinergic system in the mouse spinal cord.2015

    • Author(s)
      Sunagawa M., Shimizu-Okabe C., Kim J., Kobayashi S., and Takayama C.
    • Organizer
      第38回日本神経科学会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 玄米由来生理活性物質が担う新規の膵島機能・インスリン分泌調節機構の解明2015

    • Author(s)
      小塚智沙代、比嘉盛丈、田仲秀明、砂川澄人、植田玲、清水千草、高山千利、松下正之、親泊政一、島袋充生、益崎裕章
    • Organizer
      第58回 日本糖尿病学会年次学術集会
    • Place of Presentation
      山口
    • Year and Date
      2015-05-21 – 2015-05-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 玄米由来生理活性物質が担う新規の膵島機能・インスリン分泌調節機構の解明2015

    • Author(s)
      小塚智沙代、比嘉盛丈、田仲秀明、砂川澄人、植田玲、清水千草、高山千利、松下正之、親泊政一、島袋充生、益崎裕章
    • Organizer
      第58回 日本糖尿病学会年次学術集会
    • Place of Presentation
      海峡メッセ下関(山口県下関市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 玄米有効成分による膵内分泌機能改善の新たな分子機構2015

    • Author(s)
      小塚智沙代、砂川澄人、比嘉盛丈、池間朋己、田仲秀明、清水千草、高山千利、松下正之、親泊政一、島袋充生、益崎裕章
    • Organizer
      第52回 日本臨床分子医学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2015-04-10 – 2015-04-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 玄米有効成分による膵内分泌機能改善の新たな分子機構2015

    • Author(s)
      小塚智沙代、砂川澄人、比嘉盛丈、池間朋己、田仲秀明、清水千草、高山千利、松下正之、親泊政一、島袋充生、益崎裕章
    • Organizer
      第52回 日本臨床分子医学会学術集会
    • Place of Presentation
      みやこメッセ(京都府京都市)
    • Year and Date
      2015-04-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Kinase activity of TRPM7 regulates lipid metabolism.2015

    • Author(s)
      Katagiri C., Taku K., Shimizu C., Kozuka C., Konishi M., Tomizawa K., Takayama C., Mazuzaki H., Kozak A., and Matsushita M.
    • Organizer
      第120回日本解剖学会・第92回日本生理学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of novel target genes of mood stabilizer treatment.2015

    • Author(s)
      Takamatsu G., Shimizu C., Katagiri C., Tsumuraya T., Hayakawa T., Kondo T., and Takayama C., Matsushita M.
    • Organizer
      第120回日本解剖学会・第92回日本生理学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] GABAergic signaling in the developing CNS-GABAergic neurons and GABAA receptors.2015

    • Author(s)
      Takayama C., and Shimizu C.
    • Organizer
      第120回日本解剖学会・第92回日本生理学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Perinatal modulations of the respiration-related rhythmic activities by GABA and Cl- co-transporters2015

    • Author(s)
      Okabe A., Shimizu C., and Takayama C.
    • Organizer
      第120回日本解剖学会・第92回日本生理学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 難消化米(wx/ae)による脂肪肝及び脂質異常症の予防・改善効果2015

    • Author(s)
      Shimizu C., Kozuka C., Kobayashi C., Miyazaki Y., Kim J., Kotaniguchi M., Nakaya M., Kitamura S., Masuzaki H., and Takayama C.
    • Organizer
      第120回日本解剖学会・第92回日本生理学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 双極性障害治療の新規標的分子の同定2015

    • Author(s)
      高松岳矢、清水千草、片桐千秋、圓谷智之、早川朋子、近藤 毅、高山千利、松下正之
    • Organizer
      第36回沖縄精神神経学会
    • Place of Presentation
      沖縄
    • Year and Date
      2015-02-07
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス脊髄におけるグリシン作動性神経回路の発達変化2014

    • Author(s)
      砂川昌信、金正泰、小林しおり、清水千草、高山千利
    • Organizer
      日本解剖学会第70回九州支部学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2014-10-25
    • Related Report
      2014 Research-status Report
  • [Presentation] 高脂肪食摂取マウスにおける脂肪肝及び脂質異常症の難消化米(wx/ae)による改善効果2014

    • Author(s)
      清水千草、小塚智沙代、小林しおり、宮崎悠、金正泰、益崎裕章、高山千利
    • Organizer
      第35回日本肥満学会
    • Place of Presentation
      宮崎
    • Year and Date
      2014-10-24 – 2014-10-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Characteristic development of GABA-removal system in the mouse spinal cord2014

    • Author(s)
      Kim J., Kosaka Y., Shimizu-Okabe C., Niizaki A., and Takayama C.
    • Organizer
      韓国獣医解剖学会
    • Place of Presentation
      韓国
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report
  • [Presentation] Unique development of the GABA-removal system in the mouse spinal cord.2014

    • Author(s)
      Kim J., Kosaka Y., Shimizu-Okabe C., Nizaki A., and Takayama C.
    • Organizer
      第63回韓国解剖学会学術集会
    • Place of Presentation
      韓国
    • Year and Date
      2014-10-16 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 周産期における呼吸リズム形成に対するGABA応答性の変化2014

    • Author(s)
      清水千草、岡部明仁、高山千利
    • Organizer
      第57回日本神経化学会
    • Place of Presentation
      奈良
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
  • [Presentation] ErbB4/パルブアルブミン陽性インターニューロンを介したニューロプシンもよる海馬興奮性神経細胞の制御2014

    • Author(s)
      鈴木春満、金河大、中澤瞳、清水千草、高山千利、塩坂貞夫
    • Organizer
      第57回日本神経化学会
    • Place of Presentation
      奈良
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
  • [Presentation] Unique development of the GABA-removal system in the mouse spinal cord.2013

    • Author(s)
      Kim J, Kosaka Y, Shimizu-Okabe C, Niizaki A, Takayama C.
    • Organizer
      第63回韓国解剖学会学術集会
    • Place of Presentation
      韓国
    • Related Report
      2013 Research-status Report
  • [Presentation] GABAergic System in the hypothalamus.2013

    • Author(s)
      Shimizu-Okabe C,Kobayashi M, Niizaki A, Takayama C.
    • Organizer
      第36回内藤コンファレンス
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] Distinct development of GABA removing system in the ventral and dorsal horns in the mouse spinal cord.2013

    • Author(s)
      Takayama C, Kim J.,Kosaka Y, Shimizu-Okabe C, Niizaki A.
    • Organizer
      第36回日本神経科学大会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Developmental changes in the respiration-related rhythmic activity in mouse hypoglossal nucleus.2013

    • Author(s)
      Okabe A, Arata A, Shimizu-Okabe C, Konishi S, Fukuda A, Takayama C.
    • Organizer
      第36回日本神経科学大会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Remarks] http://www.u-ryukyu.ac.jp/

    • Related Report
      2015 Annual Research Report
  • [Remarks] http://w3.u-ryukyu.ac.jp/anatomy2/index.html

    • Related Report
      2014 Research-status Report
  • [Remarks] http://w3.u-ryukyu.ac.jp/anatomy2/index.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi