Selective destruction of the portico-thalamic feedback pathway by use of photo-oxidation technique
Project/Area Number |
25430067
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Maebashi Institute of Technology |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KATAOKA Yosuke 国立研究開発法人理化学研究所, 細胞機能評価研究チーム, チームリーダー (40291033)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 眼優位可塑性 / 両眼視 / 大脳視覚野 / 外側膝状体 / 光酸化 / フィードバック投射 / 逆行性標識 / ラット / 第一次視覚野 / アポトーシス / 感受性期 / 細胞外記録 |
Outline of Final Research Achievements |
We have developed a new activity mapping method to study the ocular dominance (OD) plasticity by applying c-Fos immunohisitochemistry. This is a powerful method to apply to rats with smaller binocular regions. To investigate the functional roles of feedback projection from the primary visual cortex to the dorsal lateral geniculate nucleus (LGN), we challenged to apply the photo-oxidation induced apoptosis (i.e. targeted apoptosis). First, we electrophysiologically mapped the LGN and injected choline e6 into the appropriate region. After waiting retrograde transport of chlorin e6 to the layer 6 neurons in V1, the laser irradiation was applied to the cortex. We found that the target was damaged by photo-oxidation induced mechanism, although the best conditions of the amount of chlorin e6 and irradiation were remained to be elucidated. By use of this technique, we first succeeded to pave the way to study the roles of V1-LGN feedback system in the regulation of OD plasticity.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Task and resting-state fMRI reveal altered salience responses to positive stimulin in patients with major depressive disorder.2016
Author(s)
Yang, Y., Zhong, N., Imamura, K., Lu, S., Li, M., Zhou, H., Li, H., Yang, X., Wan, Z., Wang, G., Hu, B., Li, K.
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Journal Title
PLOS ONE
Volume: 11
Issue: 5
Pages: 1-19
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Supranormal orientation selectivity of visual neurons in orientation-restricted animals.2015
Author(s)
Sasaki KS, Kimura R, Ninomiya T, Tabuchi Y, Tanaka H, Fukui M, Asada YC, Arai T, Inagaki M, Nakzono T, Baba M, Kato D, Nishimoto S, Sanada TM, Tani T, Imamura K, Tanaka S, Ohzawa I
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Journal Title
Sci. Rep.
Volume: 5
Issue: 1
Pages: 16712-16712
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Self-regulation of aversive emotion: a dynamic causal model, advances in computational psychophysiology.2015
Author(s)
Zhong, N., Yang, Y., Imamura, K., Lu, S., Li, M., Zhou, H., Wang, G., Li, K.
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Journal Title
Science Supplemnet
Volume: 350
Pages: 25-27
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Brain interleukin-1beta and the intrinsic receptor antagonist control peripheral toll-like receptor 3-mediated suppression of spontaneous activity in rats.2014
Author(s)
Yamato, M., Tamura, Y., Eguchi, A., Watanabe, Y., and Kataoka, Y.,
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Journal Title
PLOS ONE
Volume: 9
Issue: 3
Pages: e90950-e90950
DOI
Related Report
Peer Reviewed / Open Access
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[Book] Brain and Health Informatics2013
Author(s)
Imamura, K., Usui, S., Shirao, T, Kasmatsu, T., Schwabe, L. and Zhong, N.
Total Pages
535
Publisher
Springer
Related Report