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Role for ER stress responsive ER-Golgi SNARE in processing of Alzheimer`s disease-related protein and autophagy

Research Project

Project/Area Number 25430072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKyorin University

Principal Investigator

Suga Kei  杏林大学, 医学部, 講師 (30306675)

Co-Investigator(Renkei-kenkyūsha) MISHIMA Tatsuya  杏林大学, 医学部, 助教 (40317095)
KOFUJI Takefumi  杏林大学, 医学部, 助教 (40365200)
SAITO Ayako  杏林大学, 医学部, 実験助手 (80424109)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords神経科学 / 脳・神経 / ERストレス / アルツハイマー / オートファジー / SNARE / syntaxin / 細胞死
Outline of Final Research Achievements

Iam focusing on the role of Syntaxin 5 (Syx5), one of the ER-Golgi SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein receptor) protein, in the trafficking and processing of Alzheimer’s disease (AD)-related proteins. In this study, I examined the role of Syntaxin5 (Syx5) in the ER stress-induced autophagy and its effect on the processing of endogenous b-amyloid precursor protein (bAPP) in neuronal cells. I found that the inhibition of bAPP processing caused by ER stress was reversed by the knockdown of Syx5 expression. In addition, there was a positive correlation between the activation of the autophagy flux and the expression level of Syx5 protein. Furthermore, upregulation of Syx5 protein was important for the late stages of autophagy flux. These results suggest that, among ER-Golgi SNAREs, Syx5 is likely to have a role in mediating ER stress-induced autophagy and affecting bAPP processing in neuronal cells.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 5 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Time lapse imaging analysis of the effect of ER stress modulators on apoptotic cell assessed by caspase3/7 activation in NG108-15 cells2016

    • Author(s)
      Ayako Saito, Kei Suga, Risa Ono-Nakagawa, Masumi Sanada, and Kimio Akagawa
    • Journal Title

      Data in Brief

      Volume: 6 Pages: 20-27

    • DOI

      10.1016/j.dib.2015.11.030

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Data supporting ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid β peptide secretion2015

    • Author(s)
      Kei Suga, Ayako Saito, and Kimio Akagawa
    • Journal Title

      Data in Brief

      Volume: 5 Pages: 782-788

    • DOI

      10.1016/j.dib.2015.10.025

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Data for the effects of ER and Golgi stresses on the ER-Golgi SNARE Syntaxin5 expression and on the βAPP processing in cultured hippocampal neurons2015

    • Author(s)
      Kei Suga, Ayako Saito, Tatsuya Mishima, and Kimio Akagawa
    • Journal Title

      Data in Brief

      Volume: 5 Pages: 114-123

    • DOI

      10.1016/j.dib.2015.08.023

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ER and Golgi stresses increase ER-Golgi SNARE Syntaxin5: Implications for organelle stress and βAPP processing2015

    • Author(s)
      Kei Suga, Ayako Saito, Tatsuya Mishima, and Kimio Akagawa
    • Journal Title

      Neurosci. Lett.

      Volume: 604 Pages: 30-35

    • DOI

      10.1016/j.neulet.2015.07.017

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid β peptide secretion2015

    • Author(s)
      Kei Suga, Ayako Saito, and Kimio Akagawa
    • Journal Title

      Exp.Cell Res.

      Volume: 332 Issue: 1 Pages: 11-23

    • DOI

      10.1016/j.yexcr.2015.01.001

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] FRET-based evaluation of Bid cleavage in a single primary cultured neuron.2013

    • Author(s)
      Nakazawa H
    • Journal Title

      Neurosci Lett.

      Volume: 536 Pages: 24-28

    • DOI

      10.1016/j.neulet.2012.11.059

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Upregulation of ER-Golgi SNARE Syntaxin5 by ER stress and its relationship with βAPP processing2015

    • Author(s)
      Kei Suga, Ayako Saito, Tatsuya Mishima, and Kimio Akagawa
    • Organizer
      第58回日本神経化学会大会
    • Place of Presentation
      大宮
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 小胞輸送系の制御におけるBAG6複合体の機能解明2014

    • Author(s)
      土屋 悠吾、須賀 圭、川原 裕之
    • Organizer
      第37回日本分子生物会大会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Upregulation of ER-Golgi SNARE Syntaxin5 by ER stress and its relationship with βAPP processing2014

    • Author(s)
      Kei Suga, Ayako Saito, and Kimio Akagawa
    • Organizer
      第57回日本神経化学会大会
    • Place of Presentation
      奈良
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
  • [Presentation] ER stress suppress Abeta secretion and induces the expression of ER-Golgi SNARE including Syntaxin5 proteins in neuronal cells2013

    • Author(s)
      Suga K, Saito A, Mishima T, Akagawa K
    • Organizer
      第56回日本神経化学会大会
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Research-status Report
  • [Remarks] 杏林大学医学部細胞生理学教室ホームページ

    • URL

      http://www.kyorin-u.ac.jp/univ/faculty/medicine/labo/cell_physiology.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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