Analysis and regulation of bi-directional changes in maturation status of brain cells utilising in vitro hightrokghput assay system

• Project/Area Number: 25430077
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Fujita Health University
• Principal Investigator: KOSHIMIZU Hisatsugu 藤田保健衛生大学, 総合医科学研究所, 助教 (10610842)
• Co-Investigator(Renkei-kenkyūsha): MIYAKAWA Tsuyoshi 藤田保健衛生大学, 総合医科学研究所, 教授 (10301780)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞成熟度 / 海馬 / 歯状回 / 統合失調症 / うつ病 / モデルマウス / 精神疾患 / 神経細胞成熟度変化 / BDNF / 細胞成熟度異常 / 脳由来神経栄養因子

Outline of Final Research Achievements

We sought to determine the molecular basis of the bi-directional changes in maturation status of brain cells, utilising primary mouse hippocampal (dentate gyrus) cultures as the in vitro hightrokghput assay system. Bioinformatics was also applied for the analysis and we identified several signal molecules that can be involved in the bi-directional changes. We next screened for biological conditions that are able to lead to the changes in cellular maturation status, identifying treatments of several chemicals that may induce the maturation status changes.

Research Products

• [Journal Article] Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice (2014)
  • Author(s): Koshimizu H, Takao K, Matozaki T, Ohnishi H, Miyakawa T
  • Journal Title: PLoSOne Volume: 9 Issue: 2 Pages: e89584-e89584
  • DOI: 10.1371/journal.pone.0089584
  • Peer Reviewed / Open Access
• [Journal Article] ENU-mutagenesis mice with a non-synonymous mutation in Grin1 exhibit abnormal anxiety-like behaviors, impaired fear memory, and decreased acoustic startle response. (2013)
  • Author(s): Umemori J, Takao K, Koshimizu H, Hattori S, Furuse T, Wakana S, Miyakawa T.
  • Journal Title: BMC Res Notes Volume: 6(1) Issue: 1 Pages: 203-203
  • DOI: 10.1186/1756-0500-6-203
  • Peer Reviewed / Open Access
• [Presentation] Transcriptomic “hyper-maturity” of the hippocampus in mice (2015)
  • Author(s): Koshimizu H., Hagihara H., Ohira K., Takao T., Miyakawa T.
  • Organizer: Society for Neuroscience 45th Annual Meeting
  • Place of Presentation: 米国シカゴ
  • Year and Date: 2015-10-20
  • Int'l Joint Research
• [Presentation] Transcriptomic “hyper-maturity” of the hippocampus in mice (2015)
  • Author(s): Koshimizu H., Hagihara H., Ohira K., Takao T., Miyakawa T.
  • Organizer: 15th Annual MCCS Symposium and Poster Session
  • Place of Presentation: 米国シカゴ
  • Year and Date: 2015-10-15
  • Int'l Joint Research
• [Presentation] マウス海馬歯状回における「過成熟」現象 (2015)
  • Author(s): Koshimizu H., Hagihara H., Ohira K., Keizo Takao K., Miyakawa T.
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-30
• [Presentation] Contrasting expression paterns for immaturity and maturity marker genes in the dentate gyrus between mouse lines with ‘immature dentate gyrus’ and mice overexpressing glucocor8coid receptor (2014)
  • Author(s): Koshimizu H., Ohira K., Hagihara H., Takao T., Miyakawa T.
  • Organizer: 包括脳ネットワーク 冬のシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2014-12-12
• [Presentation] Transcriptomic Effects of Antidepressant Treatment and Glucocorticoid Receptor-overexpression on the Maturational Status of Brain Cells in Mice (2014)
  • Author(s): T Miyakawa, H Koshimizu, K Ohira, H Hagihara, R Takeuchi, K Takao
  • Organizer: American College of Neuropsychopharmacology 53rd Annual Meeting
  • Place of Presentation: Phoenix, AZ
  • Year and Date: 2014-12-09
• [Presentation] Contrasting expression patterns for immaturity and maturity marker genes in the dentate gyrus between mouse lines with ‘immature dentate gyrus’ and mice overexpressing glucocorticoid receptor (2014)
  • Author(s): Koshimizu H., Hagihara H., Takao T., Miyakawa T.
  • Organizer: Society for Neuroscience 44th Annual Meeting
  • Place of Presentation: Washington, DC
  • Year and Date: 2014-11-15
• [Presentation] Contrasting expression patterns for immaturity and maturity marker genes in the dentate gyrus between mouse lines with ‘immature dentate gyrus’ and mice overexpressing glucocorticoid receptor (2014)
  • Author(s): Koshimizu H., Hagihara H., Takao T., Miyakawa T.
  • Organizer: Annual Molecular and Cellular Cognition Society Meeting
  • Place of Presentation: Washington, DC
  • Year and Date: 2014-11-13
• [Presentation] Contrasting expression patterns for immaturity and maturity marker genes in the dentate gyrus between mouse lines with ‘immature dentate gyrus’ and mice overexpressing glucocorticoid receptor (2014)
  • Author(s): Koshimizu H., Hagihara H., Takao T., Miyakawa T.
  • Organizer: 日本神経科学学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-12
• [Presentation] Dysregulation of BDNF signaling pathway in the hippocampus of mice with “immature dentate gyrus” (2013)
  • Author(s): Koshimizu H., Ohira K., Hagihara H., Takao K., Takagi T., Kataoka M., Ishii S., Takahashi M., Miyakawa T.
  • Organizer: Annual Molecular and Cellular Cognition Society Meeting
  • Place of Presentation: 米国 San Diego
• [Presentation] Dysregulation of BDNF signaling pathway in the hippocampus of mice with “immature dentate gyrus” (2013)
  • Author(s): Koshimizu H., Ohira K., Hagihara H., Takao K., Takagi T., Kataoka M., Ishii S., Takahashi M., Miyakawa T.
  • Organizer: Society for Neuroscience 43rd Annual Meeting
  • Place of Presentation: 米国 San Diego
• [Presentation] Dysregulation of BDNF-MAPK signaling pathway in the hippocampus of mice with “immature dentate gyrus” (2013)
  • Author(s): Koshimizu H., Ohira K., Hagihara H., Takao K., Takagi T., Kataoka M., Ishii S., Takahashi M., Miyakawa T.
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 横浜
• [Book] 脳科学辞典「行動テストバッテリー」 (2013)
  • Author(s): 高雄 啓三, 小清水 久嗣, 宮川 剛
  • Total Pages: 1
  • Publisher: 脳科学辞典編集委員会
• [Remarks] Researchmap
  • URL: http://researchmap.jp/koshimizu/