human gene transgenic and knock-down rat model for HIV-1 infection

Research Project

Project/Area Number	25430084
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Laboratory animal science
Research Institution	Hokkaido University
Principal Investigator	shida Hisatoshi 北海道大学, 遺伝子病制御研究所, 客員教授 (00144395)
Co-Investigator(Kenkyū-buntansha)	Zhang Xianfeng 北海道大学, 遺伝子病制御研究所, 助教 (40374681)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords	HIV-1 / 感染 / ラットモデル / ラット / 感染モデル
Outline of Final Research Achievements	To accelerate development of anti HIV-1 vaccine and therapeutics, a rat HIV-1 infection model is useful. We have already found macrophages of rats that express human CD4, CCR5, CyclinT1 and CRM1 genes are efficiently infected with HIV-1. Here we show that the rat T cells whose CyclophilinA and Apobec3 genes are knocked out allow HIV-1 propagation.

Report

(4 results)