| Project/Area Number |
25430086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Shizuoka University |
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Principal Investigator |
KOIKE Toru 静岡大学, 理学部, 講師 (20377716)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIOJIRI Nobuyoshi 静岡大学, 理学部, 教授 (70162568)
TAGAWA Yoh-ichi 東京工業大学, 生命理工学研究科, 准教授 (70262079)
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| Research Collaborator |
ABE Naohiro
SUENAGA Koudai
Yamauchi Masashi
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 肝臓 / 肝再生 / 肝前駆細胞 / Pix-1遺伝子 / CRISPR/Cas9 / ノックアウト / マイクロアレイ / ラットES細胞 / Pdx-1遺伝子 / 相同組換え |
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| Outline of Final Research Achievements |
Proliferation of liver progenitor cells (LPCs) accompanies on many types of liver injury when the replication of hepatocytes is blocked. Although there are enormous number of researches for the characterization of LPCs, their origin and their roles in liver regeneration is still on debate. We recently found that a subpopulation of liver progenitor cells, which situate in periportal area after GalN-induced rat liver damage, express Pdx-1 gene. To address the roles of Pdx-1 in LPCs during liver regeneration, we performed overexpression and gene knockout experiments of Pdx-1 using a LPC cell line. We found that the change of Pdx-1 expression does not alter LPC proliferation. We also performed microarray analysis to compare gene expression of wild type- and knockout- LPCs for the Pdx-1 gene. The data implies that Pdx-1 is involved in the expression of neuroendocrine phenotype of LPC, as well as the suppression of hepatocyte differentiation.
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