Analysis of bone formation activity during pregnancy by in vivo imaging

• Project/Area Number: 25430088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: The University of Tokyo
• Principal Investigator: NAKANISHI Tomoko 東京大学, 医科学研究所, 助教 (10344863)
• Co-Investigator(Kenkyū-buntansha): HATSUZAWA Kiyotaka 鳥取大学, 医学部, 教授 (20256655)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オステオカルシン / 妊娠 / ルシフェラーゼ / 骨形成 / 胎児 / ホルモン / 骨代謝 / 遺伝子発現

Outline of Final Research Achievements

In OC-Luc mice, expressing luciferase under the control human osteocalcin (OC) promoter, luminescence increased bimodally during pregnancy. TRAP activity and blood concentration of estrogen/progesterone showed positive correlation with luminescence increase during pregnancy. However, expression of endogenous mouse OC decreased and did not correlate with the rise of luminescence derived from OC-Luc during pregnancy. Luminescence derived from OC-Luc was also expressed in placenta, where no expression of mouse OC has been detected. These results show that human OC promoter was controlled differently from mouse OC promoter, suggesting that OC-Luc mice reflects the human OC expression during pregnancy.

Research Products

• [Journal Article] In vivo determination of vitamin D function using transgenic mice carrying a human osteocalcin luciferase reporter gene. (2013)
  • Author(s): Nakanishi, T., Saito, R., Taniguchi, M., Oda, H., Soma, A., Yasunaga, M., Yamane, M., Sato, K.
  • Journal Title: BioMed Res. Int. Volume: 2013 Pages: 895706-895706
  • DOI: 10.1155/2013/895706
  • Peer Reviewed
• [Presentation] Bioluminescence imaging of bone formation using hairless osteocalcin-luciferase transgenic mice (2014)
  • Author(s): 中西友子
  • Organizer: 第8回Bone Research Seminar
  • Place of Presentation: 東京コンファレンスセンター(品川)
  • Invited
• [Presentation] 発光イメージングを利用したマウスにおけるリアルタイム骨形成活性評価 (2013)
  • Author(s): 中西友子
  • Organizer: 2013年国際骨代謝学会・日本骨代謝学会 第2回合同国際会議
  • Place of Presentation: 神戸国際会議場
• [Presentation] In vivo imagingを用いた妊娠期における骨形成変化の解析 (2013)
  • Author(s): 田中佑佳、中西 友子、堀直裕、初沢清隆、佐藤建三
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場