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Establishment of freezing method of rabbit embryos for cryopreservation of laboratory rabbits as human disease models

Research Project

Project/Area Number 25430091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionSaga University

Principal Investigator

KITAJIMA Shuji  佐賀大学, 総合分析実験センター, 准教授 (70284643)

Co-Investigator(Kenkyū-buntansha) NISHIJIMA Kazutoshi  秋田大学, バイオサイエンス教育研究センター, 准教授 (70435874)
MATSUHISA Fumikazu  佐賀大学, 総合分析実験センター, 助教 (20754253)
Co-Investigator(Renkei-kenkyūsha) FAN Jianglin  山梨大学, 医学工学総合研究部, 教授 (60272192)
MATSUDA Yukihisa  秋田大学, バイオサイエンス教育研究センター, 准教授 (50157327)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords凍結保存 / バイオリソース / ウサギ / 疾患モデル
Outline of Final Research Achievements

In recent years, cryopreservation by spermatozoa and embryos become an important technique to preserve strains of the laboratory animals as human disease models. In this study, we found that rabbit morula stage embryos vitrified by combination of FES (Ficoll and ethylene glycol in sucrose buffer) and special device (Cryotop) shown good in vitro development rate equally to that of non-freezing embryos. In addition to establishment of cryopreservation method in rabbit embryos, we could do endoscopic embryo transfer technique which is also necessary to preserve useful laboratory rabbit strains as human disease models.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014 2013 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (5 results)

  • [Journal Article] Motility and fertility of rabbit sperm cryopreserved using soybean lecithin as an alternative to egg yolk2015

    • Author(s)
      Nishijima, K., Kitajima, S., Koshimoto, C., Morimoto, M., Watanabe, T., Fan, J., Matsuda, Y.
    • Journal Title

      Theriogenology

      Volume: 84 Issue: 7 Pages: 1172-1175

    • DOI

      10.1016/j.theriogenology.2015.06.018

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rabbit models for the study of human atherosclerosis: from pathophysiological mechanisms to translational medicine2015

    • Author(s)
      Fan, J., Kitajima, S., Watanabe, T., Xuc, J., Zhang, J., Enqi Liu, E., Chen, Y.E.
    • Journal Title

      Pharmacology & Therapeutics

      Volume: 146 Pages: 104-119

    • DOI

      10.1016/j.pharmthera.2014.09.009

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm2014

    • Author(s)
      Kazutoshi Nishijima, Shinji Yamaguchi, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, TeruoWatanabe, Jianglin Fan, Shuji Kitajima
    • Journal Title

      Experimental Animals

      Volume: 63 Issue: 2 Pages: 149-154

    • DOI

      10.1538/expanim.63.149

    • NAID

      130003391615

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of type III antifreeze protein on sperm and embryo cryopreservation in rabbit2014

    • Author(s)
      Kazutoshi Nishijima, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, TeruoWatanabe, Jianglin Fan, Shuji Kitajima
    • Journal Title

      Cryobiology

      Volume: (in press) Issue: 1 Pages: 22-25

    • DOI

      10.1016/j.cryobiol.2014.04.014

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human apolipoprotein AII protects against diet-induced atherosclerosis in transgenic rabbits.2013

    • Author(s)
      Wang, Y.
    • Journal Title

      Arterioscler Thromb Vas Biol.

      Volume: 33 Issue: 2 Pages: 224-231

    • DOI

      10.1161/atvbaha.112.300445

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Delaying embryo development by storing at 4°C for synchronization to recipients in microinjection technique in rabbits2013

    • Author(s)
      Nishijima, K., Liu, E., Yamaguchi, S., Tanaka, M., Morimoto, M., Watanabe, T., Fan, J. and Kitajima, S.
    • Journal Title

      Lab. Anim

      Volume: 47 Issue: 1 Pages: 53-57

    • DOI

      10.1258/la.2012.012097

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 脂質代謝・動脈硬化研究におけるウサギの有用性-ヒトapoAIIトランスジェニックウサギの開発と応用-2013

    • Author(s)
      小池智也,北嶋修司,西島和俊,渡辺照男,塩見雅志,範 江林
    • Journal Title

      関西実験動物研究会会報

      Volume: 35 Pages: 67-73

    • Related Report
      2013 Research-status Report
  • [Presentation] ウサギ胚凍結におけるデバイスおよび凍結液の比較検討2016

    • Author(s)
      松久葉一、秋吉俊明、新見 学、森本正敏、範 江林、北嶋修司
    • Organizer
      第63回日本実験動物学会
    • Place of Presentation
      ミューザ川崎(神奈川)
    • Year and Date
      2016-05-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] クライオトップを用いたウサギ桑実胚の凍結における凍結保存液の検討2015

    • Author(s)
      秋吉俊明、松久葉一、江里口理嘉、森本正敏、北嶋修司
    • Organizer
      第33回九州実験動物研究会総会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Metabolomic profiling reveals muscle metabolites that are associated with favorable glucose, lipid and energy metabolism in lipoprotein lipase transgenic rabbits2015

    • Author(s)
      Nishida, Y, Nishijima, K., Mi-ichi, F., Yamada, Y., Tanaka, H., Fan, J., Kitajima, S., Tanaka, K.
    • Organizer
      5th ICCR Congress on Chronic Societal Cardiometabolic Diseases
    • Place of Presentation
      Quebec (Canada)
    • Year and Date
      2015-07-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 大豆レシチンを用いたウサギ精子の凍結法検討2014

    • Author(s)
      西島和俊、秋吉俊明、森本正敏、渡辺照男、範江林、北嶋修司
    • Organizer
      第3回ウサギバイオサイエンス研究会
    • Place of Presentation
      山梨大学
    • Year and Date
      2014-08-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 佐賀大学における遺伝子改変ウサギの開発と凍結精子による保存状況2014

    • Author(s)
      北嶋修司、西島和俊、森本正敏、渡辺照男、範 江林
    • Organizer
      第3回ウサギバイオサイエンス研究会
    • Place of Presentation
      山梨大学
    • Year and Date
      2014-08-02
    • Related Report
      2014 Research-status Report
  • [Presentation] Metabolic features of skeletal muscle in lipoprotein lipase transgenic rabbits exhibiting anti-obesity/diabetes2014

    • Author(s)
      Nishida, Y., Nishijima, K., Mi-ichi, F., Fan, J., Kitajima, S. and Tanaka, S.
    • Organizer
      10th international conference of the metaboromics society
    • Place of Presentation
      Tsuroka, Japan
    • Year and Date
      2014-06-23 – 2014-06-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 遺伝子改変ウサギの開発と維持-医学研究への利用2013

    • Author(s)
      北嶋修司、西島和俊
    • Organizer
      実験動物医学会シンポジウム
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ヒトアポリポ蛋白A-IIは動脈硬化を抑制する:新しいトランスジェニックウサギモデルの開発及び応用2013

    • Author(s)
      王 瑶、新見 学、西島和俊、Ahmed Bilal Waqar、于 瑩、小池智也、北嶋修司、範 江林
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2013 Research-status Report
  • [Presentation] ウサギの精子および胚凍結における不凍タンパク質の効果2013

    • Author(s)
      西島和俊、田中麻衣、酒井悠輔、越本知大、森本正敏、渡辺照男、範 江林、北嶋修司
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトアポリポ蛋白AII遺伝子導入ウサギの骨代謝2013

    • Author(s)
      西島和俊、増山律子、小池智也、森本正敏、渡辺照男、範 江林、北嶋修司
    • Organizer
      第2回ウサギバイオサイエンス研究会
    • Place of Presentation
      山形テルサ(山形県山形市)
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトアポリポ蛋白AII遺伝子導入ウサギの脂質代謝および骨形成能の評価2013

    • Author(s)
      西島和俊、増山律子、小池智也、山中仁木、範 江林、北嶋修司
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜県岐阜市)
    • Related Report
      2013 Research-status Report
  • [Presentation] ウサギの麻酔下採血における血清動態に関する知見・第2報2013

    • Author(s)
      河村祥介、西島和俊、川辺敏晃、福田孝規、清永智昭、井上聖也、北嶋修司
    • Organizer
      第31回九州実験動物研究会総会
    • Place of Presentation
      八千代座(熊本県山鹿市)
    • Related Report
      2013 Research-status Report
  • [Remarks] 研究活動の紹介 (佐賀大学総合分析実験センター生物資源開発部門)

    • URL

      http://www.animal.med.saga-u.ac.jp/index.php?id=2

    • Related Report
      2015 Annual Research Report
  • [Remarks] 当センターで維持している遺伝子改変ウサギ

    • URL

      http://www.animal.med.saga-u.ac.jp/index.php?id=13

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
  • [Remarks] 研究活動の紹介(佐賀大学総合分析実験センター生物資源開発部門)

    • URL

      http://www.animal.med.saga-u.ac.jp/index.php?id=2

    • Related Report
      2014 Research-status Report
  • [Remarks] 研究活動の紹介(佐賀大学総合分析実験センター生物資源開発部門ホームページ)

    • URL

      http://www.animal.med.saga-u.ac.jp/index.php?id=2

    • Related Report
      2013 Research-status Report
  • [Remarks] 施設で維持している遺伝子改変ウサギ

    • URL

      http://www.animal.med.saga-u.ac.jp/index.php?id=13

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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