| Project/Area Number |
25430093
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Keio University |
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Principal Investigator |
Aiura Koichi 慶應義塾大学, 医学部, 講師 (00184010)
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| Co-Investigator(Kenkyū-buntansha) |
Itano Osamu 慶應義塾大学, 医学部, 講師 (90265827)
Kitago Minoru 慶應義塾大学, 医学部, 講師 (70296599)
Matsuda Sachiko 慶應義塾大学, 医学部, 特任講師 (90534537)
Onishi Akira 日本大学, 生物資源科学部, 教授 (30414890)
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| Co-Investigator(Renkei-kenkyūsha) |
Kojima Misaki 独立行政法人農業生物資源研究所, 家畜ゲノム研究ユニット, 上級研究員 (80355742)
Tanigichi Yoshihito 杏林大学, 医学部, 教授 (00324616)
Yoshiura Yasushi 独立行政法人水産総合研究センター, 増養殖研究所, 研究員 (90372052)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | NASHモデル / 動物モデル / NASH |
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| Outline of Final Research Achievements |
The aim of this study was to develop a mouse model of hepatocellular carcinoma with nonalcoholic steatohepatitis that develops quickly and reflects all clinically relevant features.
Three-week-old C57BL/6J male mice were fed either a standard diet (MF) or a choline-deficient, high-fat diet (HFCD). The mice in the MF+diethylnitrosamine (DEN) and HFCD+DEN groups received a one-time intraperitoneal injection of DEN at the start of the respective feeding protocols. The mice in the HFCD and HFCD+DEN groups developed obesity early in the experiment and insulin resistance after 12 weeks. The HFCD+DEN group presented the highest incidence of nonalcoholic steatohepatitis. The levels of fibrosis and steatosis varied, but they tended to increase every 4 weeks in the HFCD and HFCD+DEN groups. Computed tomography scans indicated that all the HFCD+DEN mice developed hepatic tumors from 20 weeks, some of which were glutamine synthetase-positive.
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