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The basic research of preclinical simulation system for chemotherapy using "individual Cancer Xenografts"

Research Project

Project/Area Number 25430098
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionCentral Institute for Experimental Animals

Principal Investigator

KAWAI KENJI  公益財団法人実験動物中央研究所, その他部局等, 研究員 (30414064)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Masato  東海大学, 医学部, 教授 (00164335)
宮城 洋平  地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, その他 (00254194)
Co-Investigator(Renkei-kenkyūsha) MIYAGI Yohei  神奈川県立がんセンター, 臨床研究所, 部長 (00254194)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords疾患モデル / 個別化医療 / NOGマウス / NOG
Outline of Final Research Achievements

We established rapid and effective protocols for making individual Cancer Xenograft (iCX). In the iCXs, morphological characteristics, gene expression profiles, and genetic alteration patterns were all well preserved. In colorectal cancer iCXs, Collagen gel droplet embedded culture-drug sensitivity tests (CD-DST) were performed. The CD-DST results between original and iCX specimens were generally correlated (R2=0.01-0.89). The result of CD-DST using iCX was compatible with clinical effects of anti-cancer drug. Zinc finger protein 185 (ZNF185) is one of proteins selected from proteome analysis utilizing cancer xenograft models. We also analyzed the association between ZNF185 and the clinicopathological characteristics of colon cancer. ZNF185 expression was found to be an independent indicator of liver metastasis and prognosis in patients with colon cancer. Our iCXs with clinical information are a promising advance that may accelerate the development of anticancer therapies.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Establishment of patient-derived cancer xenografts in immunodeficient NOG mice.2015

    • Author(s)
      Chijiwa T, Kawai K, Noguchi A, Sato H, Hayashi A, Cho H, Shiozawa M, Kishida T, Morinaga S, Yokose T, Katayama M, Takenaka N, Suemizu H, Yamada R, Nakamura Y, Ohtsu T, Takano Y, Imai K, Miyagi Y, Nakamura M
    • Journal Title

      International Journal of Oncology

      Volume: 47 Issue: 1 Pages: 61-70

    • DOI

      10.3892/ijo.2015.2997

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Zinc finger protein 185 is a liver metastasis-associated factor of colon cancer.2014

    • Author(s)
      Furukawa D, Chijiwa T, Matsuyama M, Mukai M, Matsuo E, Nishimura O, Kawai K, Suemizu H, Hiraoka N, Nakagohri T, Yasuda S, Nakamura M
    • Journal Title

      Molecular and Clinical Oncology

      Volume: 2 Issue: 5 Pages: 709-713

    • DOI

      10.3892/mco.2014.298

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A versatile technique for the in vivo imaging of human tumor xenografts using near-infrared fluorochrome-conjugated macromolecule probes.2013

    • Author(s)
      Suemizu H, Kawai K, Higuchi Y, Hashimoto H, Ogura T, Itoh T, Sasaki E, Nakamura M.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 12 Pages: e82708-e82708

    • DOI

      10.1371/journal.pone.0082708

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Clinical applications of PDX/NOG models for personalized chemotherapy – possible use in chemo-sensitivity testing and clinical sequencing2016

    • Author(s)
      Chijiwa T, Isagawa T, Noguchi A, Sato H, Hayashi A, Cho H, Shiozawa M, Kishida T, Morinaga S, Yokose T, Katayama M, Takenaka N, Haraguchi M, Miyao N, Tateishi Y, Kawai K, Suemizu H, Yamada R, Nakamura Y, Imai K, Komura D, Ishikawa S, Nakamura M, Miyagi Y
    • Organizer
      AACR special conference on Patient-Derived Cancer Models
    • Place of Presentation
      ニューオーリンズ
    • Year and Date
      2016-02-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The valuable experimental model system "Cancer Xenopatient" established in NOG mice2015

    • Author(s)
      Chijiwa T, Kawai K, Noguchi A, Sato H, Hayashi A, Cho H, Shiozawa M, Kishida T, Morinaga S, Yokose T, Katayama M, Takenaka N, Suemizu H, Yamada R, Nakamura Y, Ohtsu T, Takano Y, Imai K, Miyagi Y, Nakamura M
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      フィラデルフィア
    • Year and Date
      2015-04-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NOGマウスによる高効率がん患者由来Xenograft株の樹立2013

    • Author(s)
      千々和剛、宮城洋平、川井健司、野口映、山田六平、末水洋志、高野康雄、中村雅登
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 大腸癌肝転移からのゼノグラフト作製とCD-DST法での抗がん剤感受性解析2013

    • Author(s)
      澤崎翔,塩澤学,赤池信,片山雄介,沼田幸司,樋口晃生,五代天偉,森永聡一郎,小嶋由香,川井健司,中村雅登,高野康雄,利野靖,益田宗孝,宮城洋平
    • Organizer
      第51回日本癌治療学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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