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Development of novel humanized mouse model recapiturated human allergic responses.

Research Project

Project/Area Number 25430099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionCentral Institute for Experimental Animals

Principal Investigator

Ito Ryoji  公益財団法人実験動物中央研究所, その他部局等, 研究員 (60425436)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsヒト化マウス / アレルギー / アレルギーモデル / ヒト免疫
Outline of Final Research Achievements

A purpose of this study is to develop human allergy model using humanized mice. We evaluated human allergic responses by PCA reaction usinghumanized hIL-3/GM-CSF Tg mice, which can be fully differentiated human mast cells and basophils, after administration of the serum from pollinosis patients and pollen extract.Human mast cell-mediated allergic reaction efficientry developed in the skin of Tg mice but not of nonTg mice. Furthermore, we established novel humanized mice introduced hIL-5 gene into IL-3/GM Tg mice. In lungs of these triple-Tg mice, human eosinophils has infiltrated after intratracheal administration of HDM. Therefore, these humanized mice may contribute to develop the new anti-allergic drugs.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Predominant Development of Mature and Functional Human NK Cells in a Novel Human IL-2-Producing Transgenic NOG Mouse2015

    • Author(s)
      Katano, I. Takahashi, T. Ito, R. Kamisako, T. Mizusawa, T. Ka, Y. Ogura, T. Suemizu, H. Kawakami, Y. Ito, M.
    • Journal Title

      J Immunol

      Volume: 194 Issue: 7 Pages: 3513-3525

    • DOI

      10.4049/jimmunol.1401323

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ヒト化マウスを用いたヒトアレルギーモデルの確立2015

    • Author(s)
      伊藤亮治
    • Journal Title

      アレルギーの臨床

      Volume: 478 Pages: 1164-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Visualization of the human CD4(+) T-cell response in humanized HLA-DR4-expressing NOD/Shi-scid/gammac(null) (NOG) mice by retrogenic expression of the human TCR gene2015

    • Author(s)
      Takahashi, T. Katano, I. Ito, R. Ito, M.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 456 Issue: 1 Pages: 219-224

    • DOI

      10.1016/j.bbrc.2014.11.062

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] NOD-Rag2null IL-2Rγnull mice: an alternative to NOG mice for generation of humanized mice.2014

    • Author(s)
      Katano I, Ito R, Kamisako T, Eto T, Ogura T, Kawai K, Suemizu H, Takahashi T, Kawakami Y, Ito M.
    • Journal Title

      Exp Anim.

      Volume: 63 Pages: 321-330

    • NAID

      130004677826

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effective expansion of engrafted human hematopoietic stem cells in bone marrow of mice expressing human Jagged-12014

    • Author(s)
      Negishi, N. Suzuki, D. Ito, R. Irie, N. Matsuo, K. Yahata, T. Nagano, K. Aoki, K. Ohya, K. Hozumi, K. Ando, K. Tamaoki, N. Ito, M. Habu, S.
    • Journal Title

      Exp Hematol

      Volume: 42 Issue: 6 Pages: 487-94

    • DOI

      10.1016/j.exphem.2014.02.001

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effective expansion of engrafted human hematopoietic stem cells in bone marrow of mice expressing human Jagged-12014

    • Author(s)
      Negishi N, Suzuki D, Ito R, Irie N, Matsuo K, Yahata T, Nagano K, Aoki K, Ohya K, Hozumi K, Ando K, Tamaoki N, Ito M, Habu S
    • Journal Title

      Experimental Hematology

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Establishment of a Human Allergy Model Using Human IL-3/GM-CSF-Transgenic NOG Mice.2013

    • Author(s)
      Ito, R., T. Takahashi, I. Katano, K. Kawai, T. Kamisako, T. Ogura, M. Ida-Tanaka, H. Suemizu, S. Nunomura, C. Ra, A. Mori, S. Aiso, and M. Ito.
    • Journal Title

      J Immunol

      Volume: 191 Issue: 6 Pages: 2890-2899

    • DOI

      10.4049/jimmunol.1203543

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient in vivo depletion of CD8+ T lymphocytes in common marmosets by novel CD8 monoclonal antibody administration2013

    • Author(s)
      Yoshida T, Suzuki S, Iwasaki Y, Kaneko A, Saito A, Enomoto Y, Higashino A, Watanabe A, Suzuki J, Inoue K, Kuroda T, Takada M, Ito R, Ito M, Akari H
    • Journal Title

      Immunology Letters.

      Volume: 154 Issue: 1-2 Pages: 12-17

    • DOI

      10.1016/j.imlet.2013.08.005

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Generation of human asthmatic airway inflammation model by administration of IL-33 into novel humanized IL-3/GM-CSF/IL-5 transgenic NOG mice.2016

    • Author(s)
      Ryoji Ito, Satoshi Nunomura, Ikumi Katano, Takeshi Takahashi, and Mamoru Ito
    • Organizer
      5th International Workshop on Humanized Mice
    • Place of Presentation
      チューリッヒ、スイス
    • Year and Date
      2016-01-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Intratracheal administration of IL-33 effectively induces eosinophilic airway inflammation in novel humanized NOG mice.2015

    • Author(s)
      Ryoji Ito, Satoshi Nunomura, Ikumi Katano, Takeshi Takahashi, and Mamoru Ito
    • Organizer
      日本免疫学会
    • Place of Presentation
      札幌
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Human mast cell mediated allergic responses in humanized NOG mouse expressing human IL-3 and GM-CSF2013

    • Author(s)
      Ryoji Ito, Takeshi Takahashi, Ikumi Katano, Kenji Kawai, Mamoru Ito
    • Organizer
      4th International Workshop on Humanized mice
    • Place of Presentation
      韓国・ソウル
    • Related Report
      2013 Research-status Report
  • [Presentation] IgE-mediated allergic responses in human mast cells in humanized NOG mice expressing human IL-3 and GM-CSF2013

    • Author(s)
      Ryoji Ito, Takeshi Takahashi, Ikumi Katano, Kenji Kawai, Satoshi Nunomura, Chisei Ra, Akio Mori, Sadakazu Aiso, Mamoru Ito
    • Organizer
      第15回国際免疫学会
    • Place of Presentation
      イタリア・ミラノ
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトアレルギー反応を起こすヒト化モデルマウスの開発2013

    • Author(s)
      伊藤亮治
    • Organizer
      第60回日本実験動物学会
    • Place of Presentation
      茨城・つくば市
    • Related Report
      2013 Research-status Report
  • [Presentation] 生体内ヒトアレルギー応答を可能にしたヒト化マウスの開発2013

    • Author(s)
      Ryoji Ito, Ikumi Katano, Takeshi Takahashi, Mamoru Ito
    • Organizer
      第42回日本免疫学会
    • Place of Presentation
      千葉市
    • Related Report
      2013 Research-status Report
  • [Book] Humanized Mice for HIV Research2015

    • Author(s)
      Ryoji Ito, Ikumi Katano and Mamoru Ito
    • Total Pages
      8
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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