Development of novel humanized mouse model recapiturated human allergic responses.
Project/Area Number |
25430099
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | Central Institute for Experimental Animals |
Principal Investigator |
Ito Ryoji 公益財団法人実験動物中央研究所, その他部局等, 研究員 (60425436)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | ヒト化マウス / アレルギー / アレルギーモデル / ヒト免疫 |
Outline of Final Research Achievements |
A purpose of this study is to develop human allergy model using humanized mice. We evaluated human allergic responses by PCA reaction usinghumanized hIL-3/GM-CSF Tg mice, which can be fully differentiated human mast cells and basophils, after administration of the serum from pollinosis patients and pollen extract.Human mast cell-mediated allergic reaction efficientry developed in the skin of Tg mice but not of nonTg mice. Furthermore, we established novel humanized mice introduced hIL-5 gene into IL-3/GM Tg mice. In lungs of these triple-Tg mice, human eosinophils has infiltrated after intratracheal administration of HDM. Therefore, these humanized mice may contribute to develop the new anti-allergic drugs.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Predominant Development of Mature and Functional Human NK Cells in a Novel Human IL-2-Producing Transgenic NOG Mouse2015
Author(s)
Katano, I. Takahashi, T. Ito, R. Kamisako, T. Mizusawa, T. Ka, Y. Ogura, T. Suemizu, H. Kawakami, Y. Ito, M.
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Journal Title
J Immunol
Volume: 194
Issue: 7
Pages: 3513-3525
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Effective expansion of engrafted human hematopoietic stem cells in bone marrow of mice expressing human Jagged-12014
Author(s)
Negishi, N. Suzuki, D. Ito, R. Irie, N. Matsuo, K. Yahata, T. Nagano, K. Aoki, K. Ohya, K. Hozumi, K. Ando, K. Tamaoki, N. Ito, M. Habu, S.
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Journal Title
Exp Hematol
Volume: 42
Issue: 6
Pages: 487-94
DOI
Related Report
Peer Reviewed
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[Journal Article] Effective expansion of engrafted human hematopoietic stem cells in bone marrow of mice expressing human Jagged-12014
Author(s)
Negishi N, Suzuki D, Ito R, Irie N, Matsuo K, Yahata T, Nagano K, Aoki K, Ohya K, Hozumi K, Ando K, Tamaoki N, Ito M, Habu S
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Journal Title
Experimental Hematology
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Establishment of a Human Allergy Model Using Human IL-3/GM-CSF-Transgenic NOG Mice.2013
Author(s)
Ito, R., T. Takahashi, I. Katano, K. Kawai, T. Kamisako, T. Ogura, M. Ida-Tanaka, H. Suemizu, S. Nunomura, C. Ra, A. Mori, S. Aiso, and M. Ito.
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Journal Title
J Immunol
Volume: 191
Issue: 6
Pages: 2890-2899
DOI
Related Report
Peer Reviewed
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[Journal Article] Efficient in vivo depletion of CD8+ T lymphocytes in common marmosets by novel CD8 monoclonal antibody administration2013
Author(s)
Yoshida T, Suzuki S, Iwasaki Y, Kaneko A, Saito A, Enomoto Y, Higashino A, Watanabe A, Suzuki J, Inoue K, Kuroda T, Takada M, Ito R, Ito M, Akari H
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Journal Title
Immunology Letters.
Volume: 154
Issue: 1-2
Pages: 12-17
DOI
Related Report
Peer Reviewed
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