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Development of mouse models for heteroplasmic mitochondrial DNA transmission

Research Project

Project/Area Number 25430101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

SHITARA Hiroshi  公益財団法人東京都医学総合研究所, 基盤技術研究センター, 主任基盤技術研究職員 (90321885)

Co-Investigator(Kenkyū-buntansha) YONEKAWA Hiromichi  公益財団法人東京都医学総合研究所, 基盤技術研究センター, 特任研究員 (30142110)
YAMAGUCHI Midori  公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (20593643)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsミトコンドリア / ミトコンドリアDNA / ヘテロプラズミー
Outline of Final Research Achievements

Mitochondrial DNA (mtDNA) heteroplasmy is the presence of two or more types of mtDNA molecules within single cells and individuals. To investigate the mode of heteroplasmic mtDNA transmission in mammals, heteroplasmic mtDNA mouse strains were established using cytoplasmic transfer methods by microinjection. The proportion of heteroplasmic mtDNA was measured by PCR-RFLP methods, which were improved to eliminate the influence of artifacts. Moreover, a knockout mouse strain was established using CRISPR/Cas9 system to investigate the target gene function associated with the inheritance of heteroplasmic mtDNA.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2016 2014 2013

All Presentation (5 results) (of which Invited: 1 results)

  • [Presentation] C57BL/6JおよびMus spretus由来ミトコンドリアゲノムを有する異質性マウス系統の開発2016

    • Author(s)
      設楽浩志、曹麗琴、山口碧、米川博通、多屋長治
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール(神奈川県川崎市)
    • Year and Date
      2016-05-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 哺乳類における異質性ミトコンドリアDNAの遺伝2014

    • Author(s)
      設楽 浩志
    • Organizer
      第16REG部会
    • Place of Presentation
      順天堂大学(東京都・文京区)
    • Year and Date
      2014-11-08
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] マウス初代接着性細胞におけるミトコンドリア核様体の挙動解析2013

    • Author(s)
      山口 潤也、島貫 碧、米川 博通、設楽 浩志
    • Organizer
      第60日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 1日の時間変化による組織ミトコンドリアDNA量の解析2013

    • Author(s)
      島貫 碧、米川 博通、設楽 浩志
    • Organizer
      第60日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Proportion of large deleted mitochondrial DNA in single primary adherent cells from mouse tail2013

    • Author(s)
      Midori Shimanuki, Junya Yamaguchi, Choji Taya, Hiromichi Yonekawa, Jun-Ichi Hayashi, Hiroshi Shitara
    • Organizer
      International Symposium on Mitochondria 2013: The 13th Conference of Japanese Society of Mitochondrial Research and Medicine (J-Mit)
    • Place of Presentation
      Roppongi Academyhills 49 (Tokyo, Japan)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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