| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
It has been reported that Rho signaling is involved in several cellular processes such as cell migration and cell adhesion. In cancer cells, Rho regulates cancer metastasis and tumorigenesis. However, its underlying molecular mechanism is still unknown. In this study, we examined whether mDia, which is a downstream protein of Rho, is involved in Ras-dependent tumorigenesis utilizing DMBA/TPA two-stage chemical carcinogenesis model in mice and examined activation of Ras downstream proteins such as Raf-MEK-ERK and AKT in mDia deleted cultured cells. In conclusion, it was found that the number of papilloma was significantly decreased in mDia1 KO mice. Moreover we found depletion of mDia1 caused to the suppression of MEK-ERK pathway in cultured cells. These results indicate that Rho-mDia signaling plays an important role in Ras-dependent tumorigenesis through MEK-ERK activation.
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