Elucidating the molecular mechanism of Warburg effect in cancer cells with iMRM

• Project/Area Number: 25430112
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Kyushu University
• Principal Investigator: Oshikawa Kiyotaka 九州大学, 生体防御医学研究所, 研究員 (50380051)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Keiichi 九州大学, 生体防御医学研究所, 主幹教授 (80291508)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プロテオミクス / がん代謝 / ワールブルグ効果 / がん / 質量分析計 / 癌

Outline of Final Research Achievements

The metabolic network is specific changed in cancer cells. Warburg effect (that is enhanced the glycolysis in normoxia) is first identified cancer-specific metabolic shift, but the precise mechanism is poorly understood. By comparing cancer cells with normal cells, we established cancer cell lines by oncogenes overexpression in normal diploid fibroblast. To quantify the metabolic enzyme levels in normal and cancer cell lines, we performed the in vitro proteome assisted MRM for protein absolute quantification (iMRM). We identified three metabolic enzymes which were upregulated in cancer cell lines. Overexpression of three metabolic enzymes in normal cells enhanced glucose consumption and lactate production in normoxia.

Research Products

• [Remarks] 九州大学生体防御医学研究所分子医科学分野
  • URL: http://www.bioreg.kyushu-u.ac.jp/saibou/index.html