| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The metabolic network is specific changed in cancer cells. Warburg effect (that is enhanced the glycolysis in normoxia) is first identified cancer-specific metabolic shift, but the precise mechanism is poorly understood. By comparing cancer cells with normal cells, we established cancer cell lines by oncogenes overexpression in normal diploid fibroblast. To quantify the metabolic enzyme levels in normal and cancer cell lines, we performed the in vitro proteome assisted MRM for protein absolute quantification (iMRM). We identified three metabolic enzymes which were upregulated in cancer cell lines. Overexpression of three metabolic enzymes in normal cells enhanced glucose consumption and lactate production in normoxia.
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