Construction of tumor-development mice model using Abcb10 knock out mice exhibiting PPIX accumulation

• Project/Area Number: 25430114
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Kagoshima University
• Principal Investigator: Yamamoto Masatatsu 鹿児島大学, 医歯学域医学系, 助教 (40404537)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Abcb10 / ABCB10 / PPIX / 腫瘍学発癌 / 腫瘍学発ガン

Outline of Final Research Achievements

Abcb10 is involved in the heme biosynthesis; however its exact function is unknown. We have carried out the development and analysis of Abcb10 knockout mice in order to elucidate the function.
Although an association between abcb10 depression and cancer development has been suggested, the role of abcb10 in the process of tumor progression is unclear.
We transplanted two Abcb10 Knock-down tumor cells to mice and observed. While the tumors of Abcb10 Knock-down B16 melanoma cells (P53 wild) were smaller in than those of wt ones, the tumors of Abcb10 knock-down LLC cells (P53 mutant) were larger. In the chemical carcinogenesis experiments using wt Abcb10 and Abcb10 +/- mice, the incidences of tumor development in Abcb10 +/- mice tended to be higher without statistical significance in comparison in wt Abcb10 mice.

Research Products

• [Journal Article] ATP7B expression confers multidrug resistance through drug sequestration (2015)
  • Author(s): Moinuddin FM, Shinsato Y, Komatsu M, Mitsuo R, Minami K, Yamamoto M, Kawahara K, Hirano H, Arita K, Furukawa T
  • Journal Title: Oncotarget Volume: NA Issue: 16 Pages: 22779-22790
  • DOI: 10.18632/oncotarget.8059
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ribonucleotide reductase is an effective target to overcome gemcitabine resistance in gemcitabine-resistant pancreatic cancer cells with dual resistant factors (2015)
  • Author(s): Minami K, Shinsato Y, Yamamoto M, Takahashi H, Zhang S, Nishizawa Y, Tabata S, Ikeda R, Kawahara K, Tsujikawa K, Chijiiwa K, Yamada K, Akiyama S, Torras S, Pastor-Anglada M, Furukawa T, Yasuo T.
  • Journal Title: J Pharmacol Sci Volume: 127 Issue: 3 Pages: 319-325
  • DOI: 10.1016/j.jphs.2015.01.006
  • Peer Reviewed / Open Access
• [Journal Article] Thymidine phosphorylase activates NFκB and stimulates the expression of angiogenic and metastatic factors in human cancer cells (2014)
  • Author(s): Tabata S, Ikeda R, Yamamoto M, Shimaoka S, Mukaida N, Takeda Y, Yamada K, Soga T, Furukawa T, Akiyama S
  • Journal Title: Oncotarget Volume: 5 Pages: 10473-10485
  • Peer Reviewed / Open Access
• [Journal Article] Expression of ABCB6 Is Related to Resistance to 5-FU, SN-38 and Vincristine., Anticancer Res (2014)
  • Author(s): Minami K, Kamijo Y, Nishizawa Y, Tabata S, Horikuchi F, Yamamoto M, Kawahara K, Shinsato Y, Tachiwada T, Chen ZS, Tsujikawa K, Nakagawa M, Seki N, Akiyama S, Arima K, Takeda Y, Furukawa T
  • Journal Title: Anticancer Res Volume: 34 Pages: 4767-73
  • Peer Reviewed
• [Journal Article] Establishment of a primary hepatocyte culture from the small Indian mongoose (Herpestes auropunctatus) and distribution of mercury in liver tissue (2014)
  • Author(s): Horai S, Yanagi K, Kaname T, Yamamoto M, Watanabe I, Ogura G, Abe S, Tanabe S, Furukawa T
  • Journal Title: Ecotoxicology Volume: 23 Issue: 9 Pages: 1681-9
  • DOI: 10.1007/s10646-014-1307-6
  • Peer Reviewed / Open Access
• [Journal Article] Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus casei inhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells (2014)
  • Author(s): Yukihiro Hiramatsu, Masatatsu Yamamoto, Tomomitsu Satho, Keiichi Irie, Akiko Kai, Saori Uyeda, Yuki Fukumitsu, Akihisa Toda, Takeshi Miyata, Fumio Miake, Takeshi Arakawa and Nobuhiro Kashige
  • Journal Title: BMC Biotechnology Volume: 14 Issue: 1 Pages: 1-9
  • DOI: 10.1186/1472-6750-14-38
  • Peer Reviewed / Open Access
• [Journal Article] Abcb10 role in heme biosynthesis in vivo: Abcb10 knockout in mice causes anemia with protoporphyrin IX and iron accumulation. (2014)
  • Author(s): Yamamoto M, Arimura H, Fukushige T, Minami K, Nishizawa Y, Tanimoto A, Kanekura T, Nakagawa M, Akiyama S, Furukawa T
  • Journal Title: Mol Cell Biol. Volume: 6 Issue: 6 Pages: 1077-84
  • DOI: 10.1128/mcb.00865-13
  • Peer Reviewed
• [Journal Article] Design and synthesis of prostate cancer antigen-1 (PCA-1/ALKBH3) inhibitors as anti-prostate cancer drugs. (2014)
  • Author(s): S. Nakao, M. Mabuchi, T.Shimizu, Y. Itoh, Y. Takeuchi, M. Ueda, H. Mizuno, N. Shigi, I. Ohshio, K. Jinguji, Y. Ueda, M. Yamamoto, T. Furukawa, S. Aoki, K. Tsujikawa, A. Tanaka
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 24 Issue: 4 Pages: 1071-1074
  • DOI: 10.1016/j.bmcl.2014.01.008
  • Peer Reviewed
• [Journal Article] Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells. (2014)
  • Author(s): Tajitsu Y, Ikeda R, Nishizawa Y, Mataki H, Che XF, Sumizawa T, Nitta M, Yamaguchi T, Yamamoto M, Tabata S, Akiyama S, Yamada K, Furukawa T, Takeda Y
  • Journal Title: Int J Mol Med Volume: 3 Issue: 3 Pages: 703-8
  • DOI: 10.3892/ijmm.2013.1428
  • Peer Reviewed
• [Journal Article] Molecular basis for the regulation of hypoxia-inducible factor-1α levels by 2-deoxy-D-ribose. (2014)
  • Author(s): Ikeda R, Tabata S, Tajitsu Y, Nishizawa Y, Minami K, Furukawa T, Yamamoto M, Shinsato Y, Akiyama S, Yamada K, Takeda Y
  • Journal Title: Oncol Rep Volume: 3 Issue: 3 Pages: 1444-8
  • DOI: 10.3892/or.2013.2572
  • Peer Reviewed
• [Journal Article] Reduction of MLH1 and PMS2 confers temozolomide resistance and is associated with recurrence of glioblastoma. (2013)
  • Author(s): Shinsato Y, Furukawa T, Yunoue S, Yonezawa H, Minami K, Nishizawa Y, Ikeda R, Kawahara K, Yamamoto M, Hirano H, Tokimura H, Arita K.
  • Journal Title: Oncotarget. Volume: １２ Pages: 2261-70
  • Peer Reviewed
• [Journal Article] VEGF expression is augmented by hypoxia-induced PGIS in human fibroblasts. (2013)
  • Author(s): Wang J, Ikeda R, Che XF, Ooyama A, Yamamoto M, Furukawa T, Hasui K, Zheng CL, Tajitsu Y, Oka T, Tabata S, Nishizawa Y, Eizuru Y, Akiyama S.
  • Journal Title: Int J Oncol. Volume: 3 Issue: 3 Pages: 746-54
  • DOI: 10.3892/ijo.2013.1994
  • Peer Reviewed
• [Journal Article] Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells. (2013)
  • Author(s): Suenaga M, Yamamoto M, Tabata S, Itakura S, Miyata M, Hamasaki S, Furukawa T.
  • Journal Title: Anticancer Res. Volume: 4 Pages: 1547-54
  • Peer Reviewed
• [Presentation] 『遺伝子組換え乳酸菌を用いた腸管内DDSの開発と腸管疾患への応用に向けて』 (2015)
  • Author(s): 山本雅達
  • Organizer: 霧島神経薬理フォーラム
  • Place of Presentation: 鹿児島県霧島市霧島国際ホテル
  • Year and Date: 2015-08-16
  • Invited
• [Presentation] Abcb10 knockout mice causes anemia with protoporphyrin IX and iron accumulation: Abcb10 is essential for heme biosynthesis in vivo. (2014)
  • Author(s): 山本 雅達
  • Organizer: 生体制御・創薬研究ワークショップ
  • Place of Presentation: 鹿児島
• [Presentation] Abcb10はHeme合成に必須であって、その欠損マウスはProtoporphyrin IXと鉄 の高蓄積と血球分化不全を示す (2013)
  • Author(s): 山本 雅達、有村 博史、福重 智子、河原 康一、中川 昌之、金蔵 拓郎、古川 龍彦
  • Organizer: 第８回トランスポーター研究会年会
  • Place of Presentation: 熊本
  • Invited