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Construction of tumor-development mice model using Abcb10 knock out mice exhibiting PPIX accumulation

Research Project

Project/Area Number 25430114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor biology
Research InstitutionKagoshima University

Principal Investigator

Yamamoto Masatatsu  鹿児島大学, 医歯学域医学系, 助教 (40404537)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsAbcb10 / ABCB10 / PPIX / 腫瘍学発癌 / 腫瘍学発ガン
Outline of Final Research Achievements

Abcb10 is involved in the heme biosynthesis; however its exact function is unknown. We have carried out the development and analysis of Abcb10 knockout mice in order to elucidate the function.
Although an association between abcb10 depression and cancer development has been suggested, the role of abcb10 in the process of tumor progression is unclear.
We transplanted two Abcb10 Knock-down tumor cells to mice and observed. While the tumors of Abcb10 Knock-down B16 melanoma cells (P53 wild) were smaller in than those of wt ones, the tumors of Abcb10 knock-down LLC cells (P53 mutant) were larger. In the chemical carcinogenesis experiments using wt Abcb10 and Abcb10 +/- mice, the incidences of tumor development in Abcb10 +/- mice tended to be higher without statistical significance in comparison in wt Abcb10 mice.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2015 2014 2013

All Journal Article (13 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 13 results,  Open Access: 5 results) Presentation (3 results) (of which Invited: 2 results)

  • [Journal Article] ATP7B expression confers multidrug resistance through drug sequestration2015

    • Author(s)
      Moinuddin FM, Shinsato Y, Komatsu M, Mitsuo R, Minami K, Yamamoto M, Kawahara K, Hirano H, Arita K, Furukawa T
    • Journal Title

      Oncotarget

      Volume: NA Issue: 16 Pages: 22779-22790

    • DOI

      10.18632/oncotarget.8059

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ribonucleotide reductase is an effective target to overcome gemcitabine resistance in gemcitabine-resistant pancreatic cancer cells with dual resistant factors2015

    • Author(s)
      Minami K, Shinsato Y, Yamamoto M, Takahashi H, Zhang S, Nishizawa Y, Tabata S, Ikeda R, Kawahara K, Tsujikawa K, Chijiiwa K, Yamada K, Akiyama S, Torras S, Pastor-Anglada M, Furukawa T, Yasuo T.
    • Journal Title

      J Pharmacol Sci

      Volume: 127 Issue: 3 Pages: 319-325

    • DOI

      10.1016/j.jphs.2015.01.006

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thymidine phosphorylase activates NFκB and stimulates the expression of angiogenic and metastatic factors in human cancer cells2014

    • Author(s)
      Tabata S, Ikeda R, Yamamoto M, Shimaoka S, Mukaida N, Takeda Y, Yamada K, Soga T, Furukawa T, Akiyama S
    • Journal Title

      Oncotarget

      Volume: 5 Pages: 10473-10485

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of ABCB6 Is Related to Resistance to 5-FU, SN-38 and Vincristine., Anticancer Res2014

    • Author(s)
      Minami K, Kamijo Y, Nishizawa Y, Tabata S, Horikuchi F, Yamamoto M, Kawahara K, Shinsato Y, Tachiwada T, Chen ZS, Tsujikawa K, Nakagawa M, Seki N, Akiyama S, Arima K, Takeda Y, Furukawa T
    • Journal Title

      Anticancer Res

      Volume: 34 Pages: 4767-73

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Establishment of a primary hepatocyte culture from the small Indian mongoose (Herpestes auropunctatus) and distribution of mercury in liver tissue2014

    • Author(s)
      Horai S, Yanagi K, Kaname T, Yamamoto M, Watanabe I, Ogura G, Abe S, Tanabe S, Furukawa T
    • Journal Title

      Ecotoxicology

      Volume: 23 Issue: 9 Pages: 1681-9

    • DOI

      10.1007/s10646-014-1307-6

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus casei inhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells2014

    • Author(s)
      Yukihiro Hiramatsu, Masatatsu Yamamoto, Tomomitsu Satho, Keiichi Irie, Akiko Kai, Saori Uyeda, Yuki Fukumitsu, Akihisa Toda, Takeshi Miyata, Fumio Miake, Takeshi Arakawa and Nobuhiro Kashige
    • Journal Title

      BMC Biotechnology

      Volume: 14 Issue: 1 Pages: 1-9

    • DOI

      10.1186/1472-6750-14-38

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Abcb10 role in heme biosynthesis in vivo: Abcb10 knockout in mice causes anemia with protoporphyrin IX and iron accumulation.2014

    • Author(s)
      Yamamoto M, Arimura H, Fukushige T, Minami K, Nishizawa Y, Tanimoto A, Kanekura T, Nakagawa M, Akiyama S, Furukawa T
    • Journal Title

      Mol Cell Biol.

      Volume: 6 Issue: 6 Pages: 1077-84

    • DOI

      10.1128/mcb.00865-13

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Design and synthesis of prostate cancer antigen-1 (PCA-1/ALKBH3) inhibitors as anti-prostate cancer drugs.2014

    • Author(s)
      S. Nakao, M. Mabuchi, T.Shimizu, Y. Itoh, Y. Takeuchi, M. Ueda, H. Mizuno, N. Shigi, I. Ohshio, K. Jinguji, Y. Ueda, M. Yamamoto, T. Furukawa, S. Aoki, K. Tsujikawa, A. Tanaka
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 24 Issue: 4 Pages: 1071-1074

    • DOI

      10.1016/j.bmcl.2014.01.008

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells.2014

    • Author(s)
      Tajitsu Y, Ikeda R, Nishizawa Y, Mataki H, Che XF, Sumizawa T, Nitta M, Yamaguchi T, Yamamoto M, Tabata S, Akiyama S, Yamada K, Furukawa T, Takeda Y
    • Journal Title

      Int J Mol Med

      Volume: 3 Issue: 3 Pages: 703-8

    • DOI

      10.3892/ijmm.2013.1428

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular basis for the regulation of hypoxia-inducible factor-1α levels by 2-deoxy-D-ribose.2014

    • Author(s)
      Ikeda R, Tabata S, Tajitsu Y, Nishizawa Y, Minami K, Furukawa T, Yamamoto M, Shinsato Y, Akiyama S, Yamada K, Takeda Y
    • Journal Title

      Oncol Rep

      Volume: 3 Issue: 3 Pages: 1444-8

    • DOI

      10.3892/or.2013.2572

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reduction of MLH1 and PMS2 confers temozolomide resistance and is associated with recurrence of glioblastoma.2013

    • Author(s)
      Shinsato Y, Furukawa T, Yunoue S, Yonezawa H, Minami K, Nishizawa Y, Ikeda R, Kawahara K, Yamamoto M, Hirano H, Tokimura H, Arita K.
    • Journal Title

      Oncotarget.

      Volume: 12 Pages: 2261-70

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] VEGF expression is augmented by hypoxia-induced PGIS in human fibroblasts.2013

    • Author(s)
      Wang J, Ikeda R, Che XF, Ooyama A, Yamamoto M, Furukawa T, Hasui K, Zheng CL, Tajitsu Y, Oka T, Tabata S, Nishizawa Y, Eizuru Y, Akiyama S.
    • Journal Title

      Int J Oncol.

      Volume: 3 Issue: 3 Pages: 746-54

    • DOI

      10.3892/ijo.2013.1994

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells.2013

    • Author(s)
      Suenaga M, Yamamoto M, Tabata S, Itakura S, Miyata M, Hamasaki S, Furukawa T.
    • Journal Title

      Anticancer Res.

      Volume: 4 Pages: 1547-54

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 『遺伝子組換え乳酸菌を用いた腸管内DDSの開発と腸管疾患への応用に向けて』2015

    • Author(s)
      山本雅達
    • Organizer
      霧島神経薬理フォーラム
    • Place of Presentation
      鹿児島県霧島市霧島国際ホテル
    • Year and Date
      2015-08-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Abcb10 knockout mice causes anemia with protoporphyrin IX and iron accumulation: Abcb10 is essential for heme biosynthesis in vivo.2014

    • Author(s)
      山本 雅達
    • Organizer
      生体制御・創薬研究ワークショップ
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Research-status Report
  • [Presentation] Abcb10はHeme合成に必須であって、その欠損マウスはProtoporphyrin IXと鉄 の高蓄積と血球分化不全を示す2013

    • Author(s)
      山本 雅達、有村 博史、福重 智子、河原 康一、中川 昌之、金蔵 拓郎、古川 龍彦
    • Organizer
      第8回トランスポーター研究会年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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