Investigation of discrimination and activation of Dynamin 2 GTPase by Girdin
Project/Area Number |
25430118
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor biology
|
Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
Miyoshi Hiroshi 聖マリアンナ医科大学, 医学部, 講師 (80322519)
|
Co-Investigator(Kenkyū-buntansha) |
ENOMOTO Atsushi 名古屋大学, 医学研究科, 准教授 (20432255)
YUZAWA Satoru 九州大学, 医学研究院, 助教 (40515029)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | ダイナミン / Girdin / エンドサイトーシス / 浸潤・転移 / 細胞運動 |
Outline of Final Research Achievements |
The activation mechanism of dynamin GTPase by Girdin was investigated by X-ray crystallography and confocal laser scanning microscopy. We showed that the actin-binding protein girdin is a regulator of cargo-selective CME. Girdin interacts with dynamin 2, a GTPase that excises endocytic vesicles from the plasma membrane, and functions as its GTPase activating protein. Interestingly, girdin depletion leads to the defect in clathrin-coated pit formation in the center of cells. Also, we find that girdin differentially interacts with some cargoes, which competitively prevents girdin from interacting with dynamin 2 and confers the cargo selectivity for CME.
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Report
(4 results)
Research Products
(1 results)
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[Journal Article] Regulation of cargo-selective endocytosis by dynamin 2 GTPase-activating protein girdin.2014
Author(s)
Liang Weng, Atsushi Enomoto, Hiroshi Miyoshi, Kiyofumi Takahashi, Naoya Asai, Nobuhiro Morone, Ping Jiang, Jian An, Takuya Kato, Keisuke Kuroda, Takashi Watanabe, Masato Asai, Maki Ishida-Takagishi, Yoshiki Murakumo, Hideki Nakashima, Kozo Kaibuchi and Masahide Takahashi.
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Journal Title
EMBO Journal.
Volume: 33
Issue: 18
Pages: 2098-2112
DOI
Related Report
Peer Reviewed / Open Access