Distribution of gastric epithelial cells with DNA methylation by Helicobacter pylori

• Project/Area Number: 25430127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Nara Medical University (2014-2015); National Center of Neurology and Psychiatry (2013)
• Principal Investigator: Asada Kiyoshi 奈良県立医科大学, 医学部, 研究員 (50311410)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: DNAメチル化 / 胃粘膜幹細胞 / ピロリ菌 / エピジェネティクス

Outline of Final Research Achievements

The aim of this study was to clarify aberrant methylation, induced in gastric epithelial stem cell, plays crucial roles in carcinogenesis. For that purpose, we tried to show distribution of gastric epithelial cells with DNA methylation, induced by Helicobacter pylori infection. However, methylation of a specific gene cannot be quantified in tissue sections along with visualization of their spatial distribution. Taking advantage of direct correlation between aberrant methylation and gene silencing in genes on chromosome X, we performed immunohistochemistry of genes on the chromosome X, such as FHL1 and SMARCA1. Unfortunately, specific staining patterns of these genes in gastric glands with and without aberrant methylation were not obtained.

Research Products

• [Journal Article] FHL1 on chromosome X is a single-hit gastrointestinal tumor-suppressor gene and contributes to the formation of an epigenetic field defect. (2013)
  • Author(s): Asada K, Ando T, Niwa T, Nanjo S, Watanabe N, Okochi-Takada E, Yoshida T, Miyamoto K, Enomoto S, Ichinose M, Tsukamoto T, Ito S, Tatematsu M, Sugiyama T and Ushijima T
  • Journal Title: Oncogene Volume: 32 Issue: 17 Pages: 2140-2149
  • DOI: 10.1038/onc.2012.228
  • Peer Reviewed