Investigation of tumor heterogeneity based on analyses of transcriptomic entropy

• Project/Area Number: 25430139
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Keio University
• Principal Investigator: WAKUI MASATOSHI 慶應義塾大学, 医学部, 講師 (90240465)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: がんの個性診断 / 腫瘍不均一性 / 遺伝子発現 / エントロピー / 情報理論 / 代謝経路 / トランスクリプトーム / シャノンのエントロピー / 相互情報量 / ヒトがん細胞株 / 癌

Outline of Final Research Achievements

We successfully applied information theory represented by Shannon’s entropy and mutual information to quantification of the tumor heterogeneity reflected in the transcriptome in each of biological pathways. In fact, metabolic pathways exhibiting high or low heterogeneity were detected among a number of human cancer cell lines as well as between different oxygen environments of cell culture. These results provide insights into tumor heterogeneity from the perspective of metabolic biology, suggesting the potential of diagnostic and therapeutic application.

Research Products

• [Journal Article] nnate Response to Human Cancer Cells with or without IL-2 Receptor Common γ-Chain Function in NOD Background Mice Lacking Adaptive Immunity. (2015)
  • Author(s): Nishime C, Kawai K, Yamamoto T, Katano I, Monnai M, Goda N, Mizushima T, Suemizu H, Nakamura M, Murata M, Suematsu M, Wakui M.
  • Journal Title: J Immunol Volume: 195 Issue: 4 Pages: 1883-1890
  • DOI: 10.4049/jimmunol.1402103
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bevacizumab terminates homeobox B9-induced tumor proliferation by silencing microenvironmental communication. (2014)
  • Author(s): Yoshinori Hoshino, Tetsu Hayashida, Akira Hirata, Hidena Takahashi, Naokazu Chiba, Mitsuyo Ohmura, Masatoshi Wakui, Hiromitsu Jinno, Hirotoshi Hasegawa, Shyamala Maheswaran, Makoto Suematsu, Yuko Kitagawa,
  • Journal Title: Molecular Cancer Volume: 13(1) Issue: 1 Pages: 102-102
  • DOI: 10.1186/1476-4598-13-102
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lewis Lung Carcinoma Progression Is Facilitated by TIG-3 Fibroblast Cells. (2013)
  • Author(s): Yamauchi Y, Izumi Y, Asakura K, Kawai K, Wakui M, Ohmura M, Suematsu M, Nomori H
  • Journal Title: Anticancer Res. Volume: 33 Pages: 3791-3798
  • Peer Reviewed
• [Presentation] Innate Effector Mechanisms for Elimination of Human Cancer Cells in the NOD-Background Mice Lacking Adaptive Immunity. (2016)
  • Author(s): Nishime C, Wakui M, Kawai K, Yamamoto T, Katano I, Monnai M, Goda N, Mizushima T, Suemizu H, Nakamura M, Murata M, Suematsu M
  • Organizer: 5th International Workshop on Humanized Mice (IWHM5)
  • Place of Presentation: チューリッヒ（スイス）
  • Year and Date: 2016-01-28
  • Int'l Joint Research