Search for new urothelial cancer biomarkers

• Project/Area Number: 25430140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Juntendo University
• Principal Investigator: TAKA HIKARI 順天堂大学, 医学(系)研究科(研究院), 助教 (60338374)
• Co-Investigator(Kenkyū-buntansha): FUJIMURA Tsutomu 東北医科薬科大学, 薬学部, 教授 (70245778)
• Co-Investigator(Renkei-kenkyūsha): SAITO Seiichi 琉球大学, 医学部, 教授 (80235043)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 尿中腫瘍マーカー / 糖タンパク質 / ウエスタンブロット / ELISA / 膀胱がん / 糖タンパク質抗体 / 膀胱癌 / 糖鎖修飾ペプチド抗体 / ストレプトアビジンーフィコエリスリン / 早期発見・診断 / 糖鎖修飾ペプチド / 膀胱がんの早期発見・診断

Outline of Final Research Achievements

Biomarkers from sample of serum or urine have the advantage of being less of a burden to the patients. Currently the most effective biomarker for bladder cancer is NMP22. However, NMP22 has the problem of presenting a very high rate of false positive. In this study, we examined for a new diagnostic biomarker to complement NMP22 using urine from bladder cancer patients in order to attempt early detection of bladder cancer. Protein bands detected at 75kDa (glycoprotein X) and 50kDa (glycoprotein Y) reacted strongly to SNA lectin in the urine of patients with bladder cancer. The stronger reactivity was correlated with an increase in malignancy. The sugar chains of glycoprotein X and Y are suggested the possibility as a novel biomarker which complement NMP22. Analysis of sugar chains of glycoprotein X and Y in urine will be useful tool for early diagnosis of bladder cancer.

Research Products

• [Journal Article] Absence of Elovl6 attenuates steatohepatitis but promotes gallstone formation in a (2015)
  • Author(s): Kuba M, Matsuzaka T, Matsumori R, Saito R, Kaga N, Taka H, Ikehata K, Okada N,
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 17604-17604
  • DOI: 10.1038/srep17604
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. (2015)
  • Author(s): Seko Y, Fujimura T, Yao T, Taka H, Mineki R, Okumura K, Murayama K.
  • Journal Title: Sci Rep. Volume: 8;5 Issue: 1 Pages: 13737-13751
  • DOI: 10.1038/srep13737
  • Peer Reviewed / Open Access
• [Journal Article] A clinically attainable dose of L-asparaginase targets (2015)
  • Author(s): Sugimoto K, Suzuki HI, Fujimura T, Ono A, Kaga N, Isobe Y, Sasaki M, Taka H,
  • Journal Title: Cancer Sci. Volume: 106 Issue: 11 Pages: 1534-1543
  • DOI: 10.1111/cas.12807
  • Peer Reviewed / Open Access
• [Journal Article] Effects of sitagliptin on ectopic fat contents and glucose metabolism in type 2 diabetic patients with fatty liver: A pilot study (2014)
  • Author(s): Watanabe T, Tamura Y, Kakehi S, Funayama T, Takeno K, Kawaguchi M, Yamamoto L, Sato F, Ikeda S, Taka H, Fujimura T, Fujitani Y, Kawamori R, Watada H
  • Journal Title: J Diabetes Invest Volume: Mar;6(2) Issue: 2 Pages: 164-72
  • DOI: 10.1111/jdi.12262
  • Peer Reviewed / Open Access
• [Journal Article] Increased expression of ERp57/GRP58 is protective against pancreatic beta cell death caused by autophagic failure. (2014)
  • Author(s): Yamamoto E, Uchida T, Abe H, Taka H, Fujimura T, Komiya K, Hara A, Ogihara T, Fujitani Y, Ueno T, Takeda S, Watada H.
  • Journal Title: Biochem Biophys Res Commun Volume: 453 Issue: 1 Pages: 19-24
  • DOI: 10.1016/j.bbrc.2014.09.040
  • Peer Reviewed / Open Access
• [Journal Article] A treadmill exercise reactivates the signaling of the mammalian target of rapamycin (mTor) in the skeletal muscles of starved mice. (2014)
  • Author(s): Zheng, D.M., Bian, Z., Furuya, N., Oliva Trejo, J.A., Takeda-Ezaki, M., Takahashi, K., Hiraoka, Y., Mineki, R., Taka, H., Ikeda, S., Komatsu, M., Fujimura, T., Ueno, T., Ezaki, J.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 456 Issue: 1 Pages: 519-526
  • DOI: 10.1016/j.bbrc.2014.11.118
  • Peer Reviewed / Open Access
• [Journal Article] Sialyl-glycoconjugates in cholesterol-rich microdomains of P388 cells are the triggers for apoptosis induced by Rana catesbeiana oocyte ribonuclease. (2014)
  • Author(s): Ogawa Y, Fujimura T, Ozeki Y, et al.
  • Journal Title: Glycoconj J. Volume: 31 Issue: 2 Pages: 171-84
  • DOI: 10.1007/s10719-013-9513-7
  • Peer Reviewed
• [Journal Article] Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus. (2013)
  • Author(s): Ouchi T, Fujimura T, et al.
  • Journal Title: Nat Chem Biol. Volume: 9(4):277-83 Issue: 4 Pages: 277-283
  • DOI: 10.1038/nchembio.1200
  • Peer Reviewed
• [Presentation] 妊娠中の摂取カロリー制限母ラットからの出生仔でみられたグルココルチコイドフィードバックの異常と次世代仔への影響 (2015)
  • Author(s): 根本 崇宏、藤村 務、加賀 直子、高 ひかり、上野 隆、柿沼 吉彦
  • Organizer: ＢＭＢ2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 胎生期低栄養が引き起こす血中代謝物の変動解析 (2015)
  • Author(s): 加賀 直子、高 ひかり、根本 崇宏、藤村 務、上野 隆
  • Organizer: 第9回メタボロームシンポジウム2015
  • Place of Presentation: 三島市民文化会館/三島商工会議所
  • Year and Date: 2015-09-30
• [Presentation] Search of cancer markers in exhaled breath. (2014)
  • Author(s): Fujimura T, Matsumori S , Kaga K, Taka H, Kajiyama Y, Ueno T
  • Organizer: Metabolomics 2014
  • Place of Presentation: 山形県鶴岡市 鶴岡第一ホテル・MARICA
  • Year and Date: 2014-06-23 – 2014-06-26
• [Presentation] 呼気中のがんマーカーの探索 (2013)
  • Author(s): 藤村 務、加賀 直子、松森 聖、梶山 美明、高 ひかり、上野 隆
  • Organizer: がんと代謝研究会
  • Place of Presentation: 山形県 鶴岡メタボロームキャンパス レクチャーホール
• [Presentation] Search of New Urothelial Cancer Biomarkers (2013)
  • Author(s): Tsutomu Fujimura, Hikari Taka, Saiko Kazuno, Reiko Mineki, Kimie Murayama, Takashi Ueno, Jun-ichi Furukawa, Yasuro Shinohara
  • Organizer: HUPO
  • Place of Presentation: 神奈川県 パシフィコ横浜
• [Book] 腎臓病実験法ハンドブック (2013)
  • Author(s): 富野 康日己、藤村 務、高 ひかり、他
  • Total Pages: 238
  • Publisher: 中外医学社