Biological implication and significance of microRNA in inflammatory tumorigenesis - histopathological analysis

• Project/Area Number: 25430143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Fujita Health University
• Principal Investigator: Inada Ken-ichi 藤田保健衛生大学, 医学部, 教授 (70246081)
• Co-Investigator(Kenkyū-buntansha): SHIOGAMA Kazuya 藤田保健衛生大学, 医学部, 助教 (10387699) ; Mizutani Yasuyoshi 藤田保健衛生大学, 医学部, 助教 (10546229)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: microRNA / in situ hybridization / LNA/DNAオリゴプローブ / thyramide / 病理組織切片 / 乳癌 / 扁平上皮癌 / miRNA / 慢性炎症 / 炎症性発癌 / 胃癌 / 肺癌 / 子宮頸癌 / 慢性肝炎 / 肝硬変 / 肝癌

Outline of Final Research Achievements

We examined the expressions of miR-199a and miR-200c by in situ hybridization (ISH) in hepatocellular carcinoma, lung cancer and breast cancer. We found that miR-200c expression is increased in EpCAM-positive breast cancer. We plan to reveal the molecular mechanism of the correlation between miR-200c and EpCAM. However, in this study, the expressions were very weak in almost all cases probably due to the deterioration of ISH probes. We need to improve the sensitivity and specificity of our ISH.
Our approach is unique because there are very few researchers who study the inflammation, tumorigenesis and microRNA by pathological methods. We are continuously studying the pathological relationships using a variety of tumors.

Research Products

• [Journal Article] Enzyme-labeled Antigen Method: Development and Application of the Novel Approach for Identifying Plasma Cells Locally Producing Disease-specific Antibodies in Inflammatory Lesions (2016)
  • Author(s): Mizutani Y, Shiogama K, Onouchi T, Sakurai K, Inada K, Tsutsumi Y.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 49 Issue: 1 Pages: 7-19
  • DOI: 10.1267/ahc.15030
  • NAID: 130005128330
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] New Grocott Stain without Using Chromic Acid (2015)
  • Author(s): Shiogama K, Kitazawa K, Mizutani Y, Onouchi T, Inada K, Tsutsumi Y.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 48 Issue: 1 Pages: 9-14
  • DOI: 10.1267/ahc.14045
  • NAID: 130004902930
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Coated Glass Slides TACAS Are Applicable to Heat-Assisted Immunostaining and <i>In Situ</i> Hybridization at the Electron Microscopy Level (2015)
  • Author(s): Matsui T, Onouchi T, Shiogama K, Mizutani Y, Inada K, Yu F, Hayasaka D, Morita K, Ogawa H, Mahara F, Tsutsumi Y.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 48 Issue: 5 Pages: 153-157
  • DOI: 10.1267/ahc.15012
  • NAID: 130005106598
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Application of the enzyme-labeled antigen method to visualizing plasma cells producing antibodies against Strep A, a carbohydrate antigen, of Streptococcus pyogenes in recurrent tonsillitis. (2015)
  • Author(s): Onouchi T, Mizutani Y, Shiogama K, Inada K, Okada T, Naito K, Tsutsumi Y
  • Journal Title: Microbiol. Immunol. Volume: 59 Issue: 1 Pages: 13-27
  • DOI: 10.1111/1348-0421.12213
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines. (2015)
  • Author(s): Kobayashi, K., Sakurai, K., Hiramatsu, H., Inada, K., Shiogama, K., Nakamura, S., Suemasa, F., Kobayashi, K., Imoto, S., Haraguchi, T., Ito, H., Ishizaka, A., Tsutsumi, Y., & Iba H.
  • Journal Title: Scientific Reports, Volume: 5:8428 Issue: 1 Pages: 8428-8428
  • DOI: 10.1038/srep08428
  • Peer Reviewed / Open Access
• [Journal Article] n situ visualization of plasma cells producing antibodies reactive to Porphyromonas gingivalis in periodontitis: The application of the enzyme-labeled antigen method. (2014)
  • Author(s): Mizutani Y, Tsuge S, Takeda H, Hasegawa Y, Shiogama K, Onouchi T, Inada K, Sawasaki T, Tsutsumi Y
  • Journal Title: Mol. Oral. Microbiol. Volume: 29 Pages: 156-173
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gli regulates MUC5AC transcription in human gasterointestinal cells. (2014)
  • Author(s): Kageyama-Yahara N, Yamamichi N, Takahashi Y, Nakayama C, Shiogama K, Inada K, Konno-Shimizu M, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K
  • Journal Title: Plos One. Volume: 9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis. (2013)
  • Author(s): Konno-Shimizu M., Yamamichi N., Inada K., Kageyama-Yahara N., Shiogama K., Takahashi Y., Asada-Hirayama I., Yamamichi-Nishina M., Nakayama C., Ono S., Kodashima S., Fujishiro M., Tsutsumi Y., Ichinose M., Koike K.
  • Journal Title: PLoS One. 8(2): e56766. Volume: 8
  • Peer Reviewed
• [Journal Article] Demonstration of hepatitis C virus RNA with high-sensitivity in situ hybridization employing a locked nucleic acid probe in humanized liver of infected chimeric mice and in needle biopsied human liver. (2013)
  • Author(s): Shiogama K., Inada K., Kohara M., Teramoto H., Mizutani Y., Onouchi T., Tsutsumi Y.
  • Journal Title: Int. J. Hepatol. Volume: 2013 Pages: 249535-249535
  • Peer Reviewed
• [Journal Article] High-sensitivity epidermal growth factor receptor immunostaining for colorectal carcinomas, compared with EGFR PharmDxTM: A study of diagnostic accuracy. (2013)
  • Author(s): Shiogama K., Wongsiri T., Mizutani Y., Inada K., Tsutsumi Y.
  • Journal Title: Int. J. Clin. Exp. Pathol. Volume: 6 Pages: 24-30
  • Peer Reviewed
• [Journal Article] Peach (Prunus persica) extract inhibits angiotensin II-induced signal transduction in vascular smooth muscle cells. (2013)
  • Author(s): Kono R., Okuno Y., Nakamura M., Inada K., Tokuda A., Yamashita M., Hidaka R., Utsunomiya H.
  • Journal Title: Food Chem. Volume: 139 Pages: 371-376
  • Peer Reviewed
• [Presentation] ホルモン制御性long non-coding RNA であるDRAIC は、前立腺癌の浸潤を抑制する (2016)
  • Author(s): 櫻井浩平、塩竃和也、水谷泰嘉、尾之内高慶、山道信毅、稲田健一、堤寛
  • Organizer: 第105 回 日本病理学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-05-12
• [Presentation] HE染色と免疫染色によるNETsとフィブリンの鑑別 (2016)
  • Author(s): 塩竃和也、尾之内高慶、水谷泰嘉、櫻井浩平、稲田健一、堤寛
  • Organizer: 第105回 日本病理学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-05-12
• [Presentation] 酵素抗原法の技術開発：ホルマリン固定パラフィン包埋切片作製工程が抗体活性におよぼす 影響 (2016)
  • Author(s): 水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤寛
  • Organizer: 第105回 日本病理学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-05-12
• [Presentation] 光-電子相関顕微鏡法によるNETsとフィブリンの微細構造解析 (2016)
  • Author(s): 尾之内高慶、塩竈和也、水谷泰嘉、櫻井浩平、稲田健一、堤寛
  • Organizer: 第105回 日本病理学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-05-12
• [Presentation] 新規癌抑制lncRNA nexus であるDRAIC/PCAT29 は前立腺癌の進展を制御する (2015)
  • Author(s): 櫻井浩平、稲田健一、山道信毅、堤寛
  • Organizer: 第74 回 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] SWI/SNF クロマチン構造変換因子とmiRNA の視点に立った上皮癌の遺伝子制御ネットワー クの理解 (2015)
  • Author(s): 伊庭英夫、小林和善、平松寛明、櫻井浩平、稲田健一、堤寛、原口健
  • Organizer: 第74 回 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] 好中球細胞外トラップ（NETs）とフィブリンはHE染色で鑑別できるか？ (2015)
  • Author(s): 塩竈和也、尾之内高慶、水谷泰嘉、櫻井浩平、稲田健一、堤寛
  • Organizer: 第56回 日本組織細胞化学会 総会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-10-03
• [Presentation] NETsとフィブリンの微細構造：パラフィン切片を用いた走査型電顕および免疫電顕解析 (2015)
  • Author(s): 尾之内高慶、塩竈和也、松井貴弘、水谷泰嘉、櫻井浩平、稲田健一、堤寛
  • Organizer: 第56回 日本組織細胞化学会 総会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-10-03
• [Presentation] 酵素抗原法の技術開発：ホルマリン固定パラフィン包埋切片作製過程における組織内の抗体 失活要因の検証 (2015)
  • Author(s): 水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤寛
  • Organizer: 第56回 日本組織細胞化学会 総会学術集
  • Place of Presentation: 大阪
  • Year and Date: 2015-10-03
• [Presentation] 前立腺癌の進展を制御する新規lncRNA, DRAIC の病理学的解析 (2015)
  • Author(s): 櫻井浩平、稲田健一、塩竃和也、水谷泰嘉、尾之内高慶、堤寛
  • Organizer: 第47 回 藤田学園医学会
  • Place of Presentation: 藤田保健衛生大学
  • Year and Date: 2015-10-01
• [Presentation] 好中球細胞外トラップ（NETs）とフィブリンをHE染色で鑑別する (2015)
  • Author(s): 塩竈和也、尾之内高慶、水谷泰嘉、櫻井浩平、稲田健一、堤寛
  • Organizer: 第47回 藤田学園医学会
  • Place of Presentation: 藤田保健衛生大学
  • Year and Date: 2015-10-01
• [Presentation] 好中球細胞外トラップ(NETs)とフィブリンの微細構造の違い (2015)
  • Author(s): 尾之内高慶、塩竈和也、水谷泰嘉、櫻井浩平、稲田健一、堤寛
  • Organizer: 第47 回 藤田学園医学会
  • Place of Presentation: 藤田保健衛生大学
  • Year and Date: 2015-10-01
• [Presentation] ホルマリン固定パラフィン包埋切片作製過程における抗体活性失活要因の検証～酵素抗原法 の応用をめざして (2015)
  • Author(s): 水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤寛
  • Organizer: 第47回 藤田学園医学会
  • Place of Presentation: 藤田保健衛生大学
  • Year and Date: 2015-10-01