Biological implication and significance of microRNA in inflammatory tumorigenesis - histopathological analysis
Project/Area Number |
25430143
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor diagnostics
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Research Institution | Fujita Health University |
Principal Investigator |
Inada Ken-ichi 藤田保健衛生大学, 医学部, 教授 (70246081)
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Co-Investigator(Kenkyū-buntansha) |
SHIOGAMA Kazuya 藤田保健衛生大学, 医学部, 助教 (10387699)
Mizutani Yasuyoshi 藤田保健衛生大学, 医学部, 助教 (10546229)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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Keywords | microRNA / in situ hybridization / LNA/DNAオリゴプローブ / thyramide / 病理組織切片 / 乳癌 / 扁平上皮癌 / miRNA / 慢性炎症 / 炎症性発癌 / 胃癌 / 肺癌 / 子宮頸癌 / 慢性肝炎 / 肝硬変 / 肝癌 |
Outline of Final Research Achievements |
We examined the expressions of miR-199a and miR-200c by in situ hybridization (ISH) in hepatocellular carcinoma, lung cancer and breast cancer. We found that miR-200c expression is increased in EpCAM-positive breast cancer. We plan to reveal the molecular mechanism of the correlation between miR-200c and EpCAM. However, in this study, the expressions were very weak in almost all cases probably due to the deterioration of ISH probes. We need to improve the sensitivity and specificity of our ISH. Our approach is unique because there are very few researchers who study the inflammation, tumorigenesis and microRNA by pathological methods. We are continuously studying the pathological relationships using a variety of tumors.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Coated Glass Slides TACAS Are Applicable to Heat-Assisted Immunostaining and <i>In Situ</i> Hybridization at the Electron Microscopy Level2015
Author(s)
Matsui T, Onouchi T, Shiogama K, Mizutani Y, Inada K, Yu F, Hayasaka D, Morita K, Ogawa H, Mahara F, Tsutsumi Y.
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Journal Title
ACTA HISTOCHEMICA ET CYTOCHEMICA
Volume: 48
Issue: 5
Pages: 153-157
DOI
NAID
ISSN
0044-5991, 1347-5800
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines.2015
Author(s)
Kobayashi, K., Sakurai, K., Hiramatsu, H., Inada, K., Shiogama, K., Nakamura, S., Suemasa, F., Kobayashi, K., Imoto, S., Haraguchi, T., Ito, H., Ishizaka, A., Tsutsumi, Y., & Iba H.
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Journal Title
Scientific Reports,
Volume: 5:8428
Issue: 1
Pages: 8428-8428
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Gli regulates MUC5AC transcription in human gasterointestinal cells.2014
Author(s)
Kageyama-Yahara N, Yamamichi N, Takahashi Y, Nakayama C, Shiogama K, Inada K, Konno-Shimizu M, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K
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Journal Title
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.2013
Author(s)
Konno-Shimizu M., Yamamichi N., Inada K., Kageyama-Yahara N., Shiogama K., Takahashi Y., Asada-Hirayama I., Yamamichi-Nishina M., Nakayama C., Ono S., Kodashima S., Fujishiro M., Tsutsumi Y., Ichinose M., Koike K.
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Journal Title
PLoS One. 8(2): e56766.
Volume: 8
Related Report
Peer Reviewed
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