| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we developed the novel adenoviral vector systems enabling to identify and target the cancer stem cells (CSCs), based on the adenoviral conditional targeting in stem cell (ACT-SC) method that we previouly developed. We constructed the adenoviral vectors carrying two DNA constructs: the regulatory unit expressing the Cre recombinase under the transcriptional control of a various cell-specific promoter and a switching-expression unit that under normal conditions expresses an upstream neo gene but not a reporter gene. Using glioblastoma-derived cancer stem cell population as a model, we then established the screening system of the promoters possibly responsible for cancer stemness by FACS and sphere formation analyses without harmful effects.
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