Development of the novel vector system targeting and identifying cancer stem cells for innovative cancer therapy
Project/Area Number |
25430153
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor therapeutics
|
Research Institution | Kagoshima University |
Principal Investigator |
Ijichi Nobhiro 鹿児島大学, 医歯学域医学系, 助教 (80380624)
|
Co-Investigator(Kenkyū-buntansha) |
小戝 健一郎 鹿児島大学, 医歯学域医学系, 教授 (90258418)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 腫瘍溶解性ウイルス / がん遺伝子治療 / 癌幹細胞 / アデノウイルス |
Outline of Final Research Achievements |
In this study, we developed the novel adenoviral vector systems enabling to identify and target the cancer stem cells (CSCs), based on the adenoviral conditional targeting in stem cell (ACT-SC) method that we previouly developed. We constructed the adenoviral vectors carrying two DNA constructs: the regulatory unit expressing the Cre recombinase under the transcriptional control of a various cell-specific promoter and a switching-expression unit that under normal conditions expresses an upstream neo gene but not a reporter gene. Using glioblastoma-derived cancer stem cell population as a model, we then established the screening system of the promoters possibly responsible for cancer stemness by FACS and sphere formation analyses without harmful effects.
|
Report
(5 results)
Research Products
(8 results)
-
[Journal Article] Association of positive EBAG9 immunoreactivity with unfavorable prognosis in breast cancer patients treated with tamoxifen.2013
Author(s)
Ijichi N, Shigekawa T, Ikeda K, Miyazaki T, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki S, Saeki T, Inoue S
-
Journal Title
Clin Breast Cancer
Volume: 13
Issue: 6
Pages: 465-470
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-