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The novel and comprehensive screening of genes resistant to an anticancer drug and radiation in esophageal squamous cell carcinoma

Research Project

Project/Area Number 25430159
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor therapeutics
Research InstitutionKeio University

Principal Investigator

Hirofumi Kawakubo  慶應義塾大学, 医学部, 講師 (20286496)

Co-Investigator(Kenkyū-buntansha) HAYASHIDA Tetsu  慶應義塾大学, 医学部, 助教 (80327543)
TSUTSUI Mai  慶應義塾大学, 医学部, 助教 (90573460)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords食道癌 / 抗癌剤耐性 / 放射線耐性 / トランスポゾン / 網羅的遺伝子解析 / 抗癌剤耐性遺伝子 / シスプラチン耐性 / JAK2 / シスプラチン耐性遺伝子 / 放射線耐性遺伝子 / 5FU耐性遺伝子
Outline of Final Research Achievements

Transposon is a base sequence which transports to other location in the genome at random. Transposon is activated by transposase which is enzyme in transposon its self. Activatted transposon transports to other location in the genome (cut and paste). Inserting CMV promoter to transposon as a transcriptional activator, varioius cells which have vaious overexpressed genes or knocked down genes are obtained. Using this system into esophageal squamous cell cancer (ESCC), drug or radiation was added into these cells, and the resistant colonies were harvested to detect resistant gene. This new gene screening technique was useful for detecting candidate of drug-resistant genes and radio-resistant genes in esophageal squamous cell carcinoma. We identified 37 candidate genes responsible for CDDP resistance in the two cell lines derived from ESCC cells. Eleven genes were detected as candidate radioresistant genes in the two cell lines derived from ESCC cells.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] Comprehensive screening of genes resistant to an anticancer drug in esophageal squamous cell carcinoma2015

    • Author(s)
      Tsutsui M, Kawakubo H,Hayashida T, Fukuda K, Nakamura R, Takahashi T, Wada N, Saikawa Y, Omori T, Takeuchi H, Kitagawa Y
    • Journal Title

      International Journal of Oncology

      Volume: 47 Pages: 867-874

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 食道癌抗癌剤耐性遺伝子としてJAK2遺伝子の検討2015

    • Author(s)
      気賀澤悠,川久保博文,福田和正,中村理恵子,高橋常浩,和田則仁,竹内裕也,北川雄光
    • Organizer
      第26回日本消化器癌発生学会総会
    • Place of Presentation
      米子全日空ホテル(鳥取県米子市)
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] The Novel Comprehensive Screening of Anticancer Drug Resist Genes of Esophageal Cancer.2014

    • Author(s)
      Tsutsui M, Kawakubo H, Hayashida T, Fukuda K, Takeuchi H, Kitagawa Y.
    • Organizer
      14th World Congress of International Society for Diseases of the Esophagus (ISDE)
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-09-22 – 2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] トランスポゾンを用いた網羅的遺伝子解析による食道癌放射線耐性遺伝子の同定2014

    • Author(s)
      武居友子,川久保博文,林田哲,福田和正,中村理恵子,高橋常浩,和田則仁,竹内裕也,才川義朗,大森泰,北川雄光
    • Organizer
      第52回日本癌治療学会学術集会
    • Place of Presentation
      神奈川県横浜市,パシフィコ横浜
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] トランスポゾンを用いた食道癌薬剤耐性遺伝子の網羅的解析法2014

    • Author(s)
      筒井麻衣,川久保博文,林田哲,福田和正,竹内裕也,北川雄光
    • Organizer
      第68回日本食道学会学術集会
    • Place of Presentation
      東京都文京区,東京ドームホテル
    • Year and Date
      2014-07-03 – 2014-07-04
    • Related Report
      2014 Research-status Report
  • [Presentation] トランスポゾンを用いた、薬剤耐性遺伝子の網羅的解析法

    • Author(s)
      筒井麻衣、川久保博文、林田哲、福田和正、竹内裕也、北川雄光
    • Organizer
      第22回日本がん転移学会学術集会・総会
    • Place of Presentation
      長野県,ブエナビスタ松本
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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