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Development of novel human antibody against malignant glioma-derived stem cell

Research Project

Project/Area Number 25430166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor therapeutics
Research InstitutionShizuoka Cancer Center Research Institute

Principal Investigator

Akiyama Yasuto  静岡県立静岡がんセンター(研究所), その他部局等, その他 (70222552)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords抗体医薬 / がん性幹細胞 / 悪性グリオーマ / ヒト化マウス / ヒトモノクローナル抗体
Outline of Final Research Achievements

We aimed with developing human monoclonal antibody against YKL-40 protein, which is specifically expressed on malignant glioma-derived stem cell line established at Shizuoka Cancer Center, using humanized NOG-IL-4 transgenic (TG) mice. After immunization of recombinant human YKL-40 protein to humanized NOG-IL-4 TG mice and verification of antibody titer elevation, 7 anti-YKL-40 antibody-bearing single B cells out of ten million oflymphocytes were identified and sebsequently 4 specific antibody cDNAs were successfully cloned. Finally, 3 human recombinat monoclonal antibodies against YKL-40 protein were obtained using expi293 cells transduced with antibody genes consisting of VH and VL gene. These antibodies have still weak binding affinity for YKL-40 protein, therefore affinity maturation process will be needed using a phage display system.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report

Research Products

(15 results)

All 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Effect of STAT3 inhibition on the metabolic switch in a highly STAT3-activated lymphoma cell line2015

    • Author(s)
      Akiyama Y
    • Journal Title

      Cancer Genomics Proteomics

      Volume: 12(3) Pages: 133-142

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunologically augmented skin flap as a novel dendritic cell vaccine against head and neck cancer in a rat model2015

    • Author(s)
      Inoue K, Akiyama Y et al.
    • Journal Title

      Cancer Sci

      Volume: 106 Pages: 143-150

    • DOI

      doi: 10.1111/cas.12586. Epub 2015 Feb 4

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] YKL-40 downregulation is a key factor to overcome temozolomide resistance in a glioblastoma cell line2014

    • Author(s)
      Akiyama Y
    • Journal Title

      Oncol Rep

      Volume: 32 Pages: 159-166

    • DOI

      doi: 10.3892/or.2014.3195. Epub 2014 May 16

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of the STAT3 inhibitor STX-0119 on the proliferation of a temozolomide-resistant glioblastoma cell line2014

    • Author(s)
      Ashizawa T, Akiyama Y
    • Journal Title

      Int J Oncol

      Volume: 45 Pages: 411-418

    • DOI

      doi: 10.3892/ijo.2014.2439. Epub 2014 May 12.

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] The identification of affinity peptide ligands specific to the variable region of human antibodies2014

    • Author(s)
      Akiyama Y
    • Journal Title

      Biomed Res

      Volume: 35 Pages: 105-116

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Anti-vascular endothelial growth factor receptor (VEGFR) 2 autoantibody identification in glioblastoma patient using single B cell-based antibody gene cloning2014

    • Author(s)
      Iizuka A, Akiyama Y et al.
    • Journal Title

      Immunol Lett

      Volume: 159 Pages: 15-22

    • DOI

      10.1016/j.imlet.2014.02.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients2014

    • Author(s)
      Akiyama Y et al.
    • Journal Title

      Oncol Rep

      Volume: 31 Pages: 1683-1690

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of the STAT3 inhibitor STX-0119 on the proligeration of cancer stem cells derived from glioblastoma patients2013

    • Author(s)
      Ashizawa T, Akiyama Y et al.
    • Journal Title

      Int J Oncol

      Volume: 43 Pages: 219-227

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Progect HOPE (High-tech Omics-based Patient Evaluation)-Immunosuppressive gene expression for 1,000 cancer patients2015

    • Author(s)
      秋山靖人
    • Organizer
      第74回日本癌学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-10-10 – 2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Anti-PD-1 antibody therapy using humanized MHC-dKO NOG mouse2015

    • Author(s)
      芦澤 忠
    • Organizer
      第74回日本癌学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-10-09 – 2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Autoantibody identification in glioblastoma patient using single B cell-based antibody gene cloning2014

    • Author(s)
      飯塚 明
    • Organizer
      第73回日本癌学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-09-27 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Project HOPE (High-tech Omics-based Patient Evaluation) for cancer clinics-Studies on glioblastoma cell lines2014

    • Author(s)
      秋山 靖人
    • Organizer
      第73回日本癌学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-09-26 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Effect of the STAT3 inhibitor STX-0119 on in vitro and in vivo against temozolomide-resistant glioblastoma cell line2014

    • Author(s)
      芦沢 忠
    • Organizer
      第73回日本癌学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-09-25 – 2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Effect of the STAT3 inhibitor STX-0119 on the proligeration of cancer stem cells derived from glioblastoma patients2013

    • Author(s)
      Ashizawa T, Akiyama Y et al.
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      20131003-20131003
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 抗蛍光色素モノクローナル抗体2013

    • Inventor(s)
      秋山靖人、飯塚 明
    • Industrial Property Rights Holder
      静岡県
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特願2013-248159
    • Filing Date
      2013-11-29
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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