Identification of chromatin factor which is associated with neuron specific chromatin dynamics.
| Project/Area Number |
25430167
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Kanazawa University |
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Principal Investigator |
Horike Shin-ichi 金沢大学, 学際科学実験センター, 准教授 (40448311)
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| Co-Investigator(Kenkyū-buntansha) |
MEGURO Makiko 金沢大学, 学際科学実験センター, 博士研究員 (20304222)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKABAYASHI Kazuhiko 国立成育医療研究センター, 周産期病態研究部, 室長 (10415557)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エピジェネティクス / インプリンティング / クロマチン / DNA-FISH / ゲノムインプリンティング / クロマチン構造 / 転写マシナリー / 染色体テリトリー / クロマチンダイナミクス / 3Cアッセイ / 非コードRNA / siRNA |
|---|
| Outline of Final Research Achievements |
The PWS/AS imprinted gene cluster on human chromosome 15q11-q13 spans ~4Mb region that includes several protein-coding genes and the 450kb long UBE3A-ATS lncRNA. UBE3A-ATS is expressed from its unmethylated CpG island promoter contained in the defined ICR on the paternal chromosome. Despite the fact that PWS-IC is known to be necessary for the coordinately controlled gene expression of 15q11-q13 imprinted domain, the mechanism by which it act and how it influence to the several protein-coding genes over long distance, is unknown. In order to define the precise long-range gene regulation of 15q11-q13, we examined shRNA-based knockdown experiments by using the human/mouse hybrid cells which is containing a single human chromosome of identified parental origin. Our results support the idea that Methyl-CpG binding proteinsuch as MeCP2, Mbd1 have an essential role for long-range gene regulation of 15q11-q13.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Oxytocin for Male Subjects with Autism Spectrum Disorder and Comorbid Intellectual Disabilities: A Randomized Pilot Study2016
Author(s)
Toshio Munesue, Hiroyuki Nakamura, Mitsuru Kikuchi, Yui Miura, Noriyuki Takeuchi, Tokie Anme, Eiji Nanba, Kaori Adachi, Kiyotaka Tsubouchi, Yoshimichi Sai, Ken-ichi Miyamoto, Shin-ichi Horike, Shigeru Yokoyama, Hideo Nakatani, Yo Niida, Hirotaka Kosaka, Yoshio Minabe and Haruhiro Higashida
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Journal Title
Frontiers in Psychiatry
Volume: 7-2
Pages: 2-2
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation.2016
Author(s)
Guo, L., Yamashita, H., Kou, I., Takimoto, A., Meguro-Horike, M., Horike, S., Adachi, T., Ikegawa, S., Hiraki, Y., Shukunami, C.
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Journal Title
Plos Genetics
Volume: 12
Issue: 1
Pages: e1005802-e1005802
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] 核内ダイナミクスと遺伝子発現2014
Author(s)
堀家慎一
Organizer
名市大エピジェネティクス研究会
Place of Presentation
ホテル木曽路（木曽郡南木曽町，長野県）
Year and Date
2014-09-04 – 2014-09-05
Related Report
Invited
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