Methods and strategies to determine epigenetic variation in human disease: association with R/G-band boundaries on human chromosomes
| Project/Area Number |
25430168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | University of Shizuoka (2016)
Hamamatsu University School of Medicine (2013-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
前川 真人 浜松医科大学, 医学部, 教授 (20190291)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 染色体バンド / DNA複製 / 染色体バンド境界 / DNA複製タイミング / 神経可塑性 / 脳神経疾患 / ヒトゲノム / 染色体 / バンド境界 / 疾患遺伝子 |
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| Outline of Final Research Achievements |
The human genome is composed of large-scale compartmentalized structures resulting from variations in the amount of guanine and cytosine residues (GC%) and in the timing of DNA replication. These compartmentalized structures are related to the light- and dark-staining bands along chromosomes after the appropriate staining. We propose that R/G-chromosomal boundaries, which correspond to regions showing a switch in replication timing from early to late S phase (early/late-switch regions) and of transition in GC%, have an extremely low number of replication origins and more non-B-form DNA structures than other genomic regions. Further, we suggest that genes located at R/G boundaries and which contain such DNA sequences have an increased risk of genetic instability and of being associated with human diseases. Finally, we propose strategies for genome and epigenome analyses based on R/G boundaries.
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Report
(5 results)
Research Products
(11 results)
