| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Boundary sequences within non-coding region of genomes are important elements to ensure the proper gene structure and expressions by blocking inappropriate interactions between neighboring genes. In this research, we showed that physical properties of DNA molecule play an important role in the activity of the Ars insulator, which was identified in sea urchin arylsulfatase gene. In analyses of DNA physical properties of artificially synthesized trinucleotide repeats revealed that the property of AAT repeat is similar to that of the Ars insulator. Furthermore, mathematical analysis of ChIP-seq data suggested that surrounding sequence of AAT repeats may have regularly spaced array of nucleosomes. Moreover, during the process of this research, we constructed simple elastic network models for exhaustive analysis of long double-stranded DNA dynamics with sequence geometry dependence.
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