| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
PCNA is loaded on chromatin by PCNA loading RFC complex upon initiation of S phase and acts as a platform for a large number of proteins involved not only in DNA replication, but also in replication-linked various processes. To date, three RFC complexes related with PCNA were identified. RFC1-RFC and Ctf18-RFC, function as a PCNA loader, loads PCNA onto DNA. Elg1-RFC also relates with PCNA, but its physiological role in PCNA regulation on chromatin remained unclear.
In this study, PCNA levels on chromatin were increased in Elg1-depleted cells. Various chromatin-associated protein levels on chromatin were affected, and in mitosis, cells with aberrant and lagging chromosomes were frequently detected. These findings suggest that Elg1-RFC has an important role in maintaining chromosome integrity by regulating PCNA levels on chromatin, thereby acting as a PCNA unloader. Our findings demonstrate that Elg1-RFC have unique roles for gemone integrity by controlling PCNA on chromatin.
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