cell cycle regulation and genome integrity by novel PCNA unloading mechanism
| Project/Area Number |
25430171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | University of Hyogo |
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Principal Investigator |
SHIOMI Yasushi 兵庫県立大学, 生命理学研究科, 准教授 (80380567)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | PCNA / RFC複合体 / PCNAローディング / PCNAアンローディング / 細胞周期制御 / ゲノム維持 / Elg1-RFC / PCNAローダー / PCNAアンローダー / 細胞周期 / 複製 |
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| Outline of Final Research Achievements |
PCNA is loaded on chromatin by PCNA loading RFC complex upon initiation of S phase and acts as a platform for a large number of proteins involved not only in DNA replication, but also in replication-linked various processes. To date, three RFC complexes related with PCNA were identified. RFC1-RFC and Ctf18-RFC, function as a PCNA loader, loads PCNA onto DNA. Elg1-RFC also relates with PCNA, but its physiological role in PCNA regulation on chromatin remained unclear.
In this study, PCNA levels on chromatin were increased in Elg1-depleted cells. Various chromatin-associated protein levels on chromatin were affected, and in mitosis, cells with aberrant and lagging chromosomes were frequently detected. These findings suggest that Elg1-RFC has an important role in maintaining chromosome integrity by regulating PCNA levels on chromatin, thereby acting as a PCNA unloader. Our findings demonstrate that Elg1-RFC have unique roles for gemone integrity by controlling PCNA on chromatin.
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Report
(4 results)
Research Products
(20 results)
