| Project/Area Number |
25430172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Yuji 東邦大学, 理学部, 講師 (70362522)
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| Research Collaborator |
KUWATA Yusuke 東邦大学, 理学部, 博士研究員 (00772708)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 全ゲノム / 比較ゲノム / 生殖細胞ゲノム / 体細胞ゲノム / 染色体放出 / ゲノム再編成 / 次世代シーケンシング / ヌタウナギ / 全ゲノム解析 / 次世代シーケンサー / 比較ゲノミクス |
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| Outline of Final Research Achievements |
In inshore hagfish, Eptatretus burgeri, it is known that chromosome elimination occurs during early embryogenesis, and shown that the eliminated chromosomes are mosaics of highly repetitive, germline-restricted DNA families. In this study, using the next-generation sequencing technology, whole DNA sequences of somatic and germline genomes in this specie were determined. The current assembly constructed 94,650 scaffolds (>500bp) in somatic, and 83,538 scaffolds (>500bp) in germline genomes, subsequently the genome size of somatic and germline genomes were estimated to be 1.77 Gb and 2.09 Gb, from the k-mer (k=40) analysis, indicating the similar values calculated by previous studies. At present, the germline-restricted sequences, namely the eliminated genome is determined and investigated by the mapping of RNA-seq data and annotated the transcriptional regions.
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