Identification of Regulatory Roles of All Transcription Factors from a Single Organism

• Project/Area Number: 25430173
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Hosei University
• Principal Investigator: ISHIHAMA Akira 法政大学, マイクロ・ナノテクノロジー研究センター, 研究員 (80019869)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 転写因子 / RNAポリメラーゼ / シグマ因子 / ゲノム制御 / 転写調節 / 大腸菌 / SELEX / 転写制御 / ゲノム / 遺伝子 / プロモータ－ / 制御ネットワーク

Outline of Final Research Achievements

The genome of the model prokaryote Escherichia coli contains approximately 4,500 genes. Expression pattern of the genome is determined through selective transcription by the RNA polymerase. Its gene selectivity is controlled at two steps through interaction with seven sigma factors and about 300 transcription factors. For identification of regulatory targets by transcription regulators, we identified their binding sites on the E. coli genome using Genomic SELEX system. We have so far identified the regulatory targets for all seven sigma factors and for more then 250 transcription factors. The results altogether are assembled into a single database TEC (Transcription Profile of Escherichia coli) (www.shigen.nig.ac.jp/ecoli/tec/), which is now open to public through NIG (National Institute of Genetics). Noteworthy is that this is the first success of identification of the regulatory roles of the whole set of transcription regulators form a single organism.

Research Products

• [Journal Article] Transcription profile of Escherichia coli: Genomic SELEX search for regulation targets of transcription factors. (2016)
  • Author(s): Ishihama, A., Shimada, T. and Yamazaki, Y.
  • Journal Title: Nucleic Acids Research Volume: 44 Issue: 5 Pages: 2058-2074
  • DOI: 10.1093/nar/gkw051
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcription factor CecR (YbiH) regulates a set of genes affecting the sensitivity of Escherichia coli against cefoperazone and chloramphenicol (2016)
  • Author(s): Yamanaka, Y., Shimada, T., Yamamoto, K. and Ishihama, A.
  • Journal Title: Microbiology Volume: 162 Issue: 7 Pages: 1253-1264
  • DOI: 10.1099/mic.0.000292
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Strength and regulation of seven rRNA promoters in Escherichia coli (2015)
  • Author(s): Maeda, M., Shimada, T. and Ishihama, A.
  • Journal Title: PLoS ONE Volume: 10 Pages: 0144697-0144697
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of transcription factor NimR (YeaM) in sensitivity control of Escherichia coli to 2-nitroimidazole (2015)
  • Author(s): Ogasawara, H., Ohe, S. and Ishihama, A.
  • Journal Title: FEMS Microbiology Letters Volume: 362 Issue: 1 Pages: 1-8
  • DOI: 10.1093/femsle/fnu013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Three members of polyamine modulon under oxidative stress conditions: Two transcription factors (SoxR and EmrR) and a glutathione synthase enzyme (GshA) (2015)
  • Author(s): Sakamoto, A., Terui, Y., Yoshida, T., Yamamoto, T., Suzuki, H., Yamamoto, K., Ishihama A., Igarashi, K. and Kashiwagi, K.
  • Journal Title: PLoS ONE Volume: 10 Issue: 4 Pages: e0124883-e0124883
  • DOI: 10.1371/journal.pone.0124883
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expanded roles of Lrp in transcription regulation of the Eschericia coli genome: Genomic SELEX screening of the regulation targets (2015)
  • Author(s): Shimada, T., Saito, N., Maeda, M., Tanaka, K. and Ishihama, A
  • Journal Title: Microbial Genomics Volume: 1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expanded roles of two-component response regulator OmpR in Escherichia coli: genomic SELEX search for novel regulation targets. (2015)
  • Author(s): Shimada T, Takada H, Yamamoto K, Ishihama A.
  • Journal Title: Genes to Cells Volume: 11 Issue: 11 Pages: 915-931
  • DOI: 10.1111/gtc.12282
  • Peer Reviewed / Open Access
• [Journal Article] Growth phase dependent changes in the structure and protein composition of nucleoid in Escherichia coli (2015)
  • Author(s): Talukder, A.A. and Ishihama, A
  • Journal Title: Science China Life Science Volume: 58 Issue: 9 Pages: 902-911
  • DOI: 10.1007/s11427-015-4898-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cooperative regulation of the common target genes between hydrogen peroxide-response YedVW and copper-response CusSR in Escherichia coli. (2015)
  • Author(s): Urano, H., Yamamoto, Y., Ishihama, A. and Ogasawara, H.: Cooperative regulation of the common target genes between hydrogen peroxide-response YedVW and copper-response CusSR in Escherichia coli.
  • Journal Title: Microbiology Volume: 161 Issue: 4 Pages: 729-738
  • DOI: 10.1099/mic.0.000026
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Induction of the Escherichia coli yijE gene expression by involved in cystine (2015)
  • Author(s): Yamamoto, K., Nonaka, G., Ozawa, T., Nakanishi, K. and Ishihama, A.
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 79 Issue: 2 Pages: 601-612
  • DOI: 10.1080/09168451.2014.972328
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cross-talk in promoter recognition between six NalL-family response regulators of Escherichia coli two-component system (2015)
  • Author(s): Yoshida, M., Ishihama, A. and Yamamoto, K
  • Journal Title: Genes Cells Volume: 20 Issue: 7 Pages: 601-612
  • DOI: 10.1111/gtc.12251
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulatory role of transcription factor SutR (YdcN) in sulfur utilization. (2014)
  • Author(s): Yamamoto, K., Nakano, M. and Ishihama, A.
  • Journal Title: Microbiology Volume: 161 Issue: 1 Pages: 99-111
  • DOI: 10.1099/mic.0.083550-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing. (2014)
  • Author(s): Conway T, Creecy JP, Maddox SM, Grissom JE, Conkle TL, Shadid TM, Teramoto J, San Miguel P, Shimada T, Ishihama A, Mori H, Wanner BL.
  • Journal Title: MBio Volume: 5 Issue: 4
  • DOI: 10.1128/mbio.01442-14
  • Peer Reviewed / Open Access
• [Journal Article] Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation. (2014)
  • Author(s): Dudin O, Geiselmann J, Ogasawara H, Ishihama A, Lacour S.
  • Journal Title: Journal of Bacteriology Volume: 196 Issue: 3 Pages: 707-715
  • DOI: 10.1128/jb.00938-13
  • Peer Reviewed / Open Access
• [Journal Article] Intracellular concentrations of transcription factors in Escherichia coli: 65 species with known regulatory functions. (2014)
  • Author(s): Ishihama, A., Kori, A., Koshio, E., Yamada, K., Maeda, H., Shimada, T., Makinoshima, H., Iwata, A. and Fujita, N.
  • Journal Title: journal of Bacteriology Volume: 196 Pages: 2718-2727
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of cAMP-CRP in transcription activation and repression of the pck gene encoding PEP carboxykinase, the key enzyme of gluconeogenesis. (2014)
  • Author(s): Nakano, M., Ogasawara, H., Shimada, T., Yamamoto, K. and Ishihama, A.
  • Journal Title: FEMS Microbiology Letters Volume: 355 Issue: 2 Pages: 93-99
  • DOI: 10.1111/1574-6968.12466
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Roles of cell division control factor SdiA: recognition of quorum sensing signals and modulation of transcription regulation targets (2014)
  • Author(s): Tomohiro Shimada1,2,3, Kaori Shimada, Makoto Matsui, Yuichi Kitai, Jun Igarashi, Hiroaki Suga, Akira Ishihama
  • Journal Title: Genes to Cells Volume: 19 Issue: 5 Pages: 405-418
  • DOI: 10.1111/gtc.12139
  • Peer Reviewed / Open Access
• [Journal Article] The whole set of constitutive promoters recognized by RNA polymerase RpoD holoenzyme of Escherichia coli (2014)
  • Author(s): Tomohiro Shimada, Yukiko Yamazaki, Kan Tanaka and Akira Ishihama
  • Journal Title: PLos One Volume: 9 Issue: 3 Pages: e90447-e90447
  • DOI: 10.1371/journal.pone.0090447
  • Peer Reviewed
• [Journal Article] Role for σ38 in prolonged survival of Escherichia coli in Drosophila melanogaster. (2014)
  • Author(s): Shiratsuchi, A., Shimamoto, N., Nitta, M., Tuan, T. Q., Firdausi, A., Gawasawa, M., Yamamoto, K., Ishihama, A., and Nakanishi, Y.
  • Journal Title: J. Immunol. Volume: 192 Issue: 2 Pages: 666-675
  • DOI: 10.4049/jimmunol.1300968
  • Peer Reviewed / Open Access
• [Journal Article] Induction of the Escherichia coli yijE gene expression by involved in cystine. (2014)
  • Author(s): Yamamoto, K., Nonaka, G., Ozawa, T., Nakanishi, K. and Ishihama, A.
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 27 Pages: 1-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression levels of transcription factors in Escherichia coli: Growth phase-dependent variation of 94 regulators from 7 families. (2014)
  • Author(s): Yamamoto, K., Watanabe, H. and Ishihama, A.
  • Journal Title: Microbilogy Volume: 160 Issue: 9 Pages: 1903-1913
  • DOI: 10.1099/mic.0.079889-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Role of YdeO regulator for Escherichia coli response to acidic and anaerobic stresses. (2014)
  • Author(s): Yamanaka, Y., Oshima, T., Ishihama, A. and Yamamoto, K.
  • Journal Title: PLoS ONE Volume: 9 Issue: 11 Pages: e111962-e111962
  • DOI: 10.1371/journal.pone.0111962
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Roles of cell division control factor SdiA: Recognition of quorum sensing signals and modulation of transcription regulation targets (2014)
  • Author(s): Shimada, T., Shimada, K., Matsui, M., Kitai, Y., Igarashi, J., Suga, H. and Ishihama, A.
  • Journal Title: Genes Cells Volume: 19
  • Peer Reviewed
• [Journal Article] Role of σ38 in prolonged survival of Escherichia coli in Drosophila melanogaster (2014)
  • Author(s): Shiratsuchi, A., Shimamoto, N., Tusan, T.Q., Mitta, M., Firdausi, A., Nagasawa, M., Yamamoto, K., Ishihama, A. and Nakanishi, Y.
  • Journal Title: J. Immunol. Volume: 192 Pages: 666-675
  • Peer Reviewed
• [Journal Article] Identification of the set of genes, including nonannotated morA, under the direct control of ModE in Escherichia coli. (2013)
  • Author(s): Kurata T, Katayama A, Hiramatsu M, Kiguchi Y, Takeuchi M, Watanabe T, Ogasawara H, Ishihama A, Yamamoto K.
  • Journal Title: Journal of Bacteriology Volume: 195 Issue: 19 Pages: 4496-4505
  • DOI: 10.1128/jb.00304-13
  • Peer Reviewed
• [Journal Article] The nucleoid-associated protein Dan organizes chromosomal DNA through rigid nucleoprotein filament formation in Escherichia coli during anoxia. (2013)
  • Author(s): Lim, C.J., Lee, S.Y., Teramoto, J., Ishihama, A. and Yan, J
  • Journal Title: Nucleic Acids Res. Volume: 41 Issue: 2 Pages: 746-753
  • DOI: 10.1093/nar/gks1126
  • Peer Reviewed
• [Journal Article] Involvement of EnvZ-OmpR two-component system in virulence control of Escherichia coli in Drosophila melanogaster. (2013)
  • Author(s): Pullkay, P., Nakanishi, Y, Nitta, M., Yamamoto, K., Ishihama, A., and Shiratsuchi, A.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 438 Issue: 2 Pages: 306-311
  • DOI: 10.1016/j.bbrc.2013.07.062
  • Peer Reviewed
• [Journal Article] Structural change of DNA induced by nucleoid proteins: Growth phase-specific Fis and stationary phase-specific Dps (2013)
  • Author(s): Yuko T. Sato, Shun N. Watanabe, Takahiro Kenmotsu, Masatoshi Ichikawa, Yuko Yoshikawa, Jun Teramoto, Tadayuki Imanaka, Akira Ishihama and Kenichi Yoshikawa
  • Journal Title: Biophysical Journal Volume: 105 Issue: 4 Pages: 1037-1044
  • DOI: 10.1016/j.bpj.2013.07.025
  • Peer Reviewed
• [Journal Article] Involvement of the ribose operon repressor RbsR in regulation of purine nucleotide synthesis in Escherichia coli (2013)
  • Author(s): Shimada, T., Kori, A. and Ishihama, A.
  • Journal Title: FEMS Microbiol. Lett. Volume: 344 Issue: 2 Pages: 159-165
  • DOI: 10.1111/1574-6968.12172
  • Peer Reviewed
• [Journal Article] Screening of promoter-specific transcription factors: Multiple regulators for the sdiA gene involved in cell differentiation and quorum sensing (2013)
  • Author(s): Shimada, K., Ogasawara, H., Yamada, K., Shimura, M., Kori, A., Shimada, T., Yamanaka,Y., Yamamoto, K. and Ishihama, A.
  • Journal Title: Microbiology Volume: 159 Pages: 2501-2512
  • Peer Reviewed
• [Journal Article] Regulation of the rmf gene encoding ribosome modulation factor in Escherichia coli: Involvement of cAMP receptor protein (2013)
  • Author(s): Shimada, T., Yoshida, H. and Ishihama, A.
  • Journal Title: J. Bacteriol. Volume: 195 Issue: 10 Pages: 2212-2219
  • DOI: 10.1128/jb.02279-12
  • Peer Reviewed
• [Journal Article] バイオフィルム形成に関わる遺伝子群の転写制御 (2013)
  • Author(s): 石浜 明
  • Journal Title: Bacterial Adherence & Biofilm Volume: 26 Pages: 5-8
• [Journal Article] A novel regulator PgrR for switch control of peptideglycan recycling in Escherichia coli. (2012)
  • Author(s): Shimada, T., Yamazaki, K. and Ishihama, A.
  • Journal Title: Genes to Cells Volume: 18 Issue: 2 Pages: 123-134
  • DOI: 10.1111/gtc.12026
  • Peer Reviewed
• [Presentation] 大腸菌バイオフィルム形成統括因子CsgDの新規発現抑制機構の解明 (2016)
  • Author(s): 石塚俊行、太田あず佐、石浜 明、小笠原 寛
  • Organizer: 第10回日本ゲノム微生物学会年会
  • Place of Presentation: 東工大・大岡山、東京都目黒区
  • Year and Date: 2016-03-04
• [Presentation] Milestone toward Understanding Bacterial Genome Regulation (2015)
  • Author(s): Ishihama, A.
  • Organizer: Asian Conference in Transcription 2015
  • Place of Presentation: National University Sigapore, Singapore
  • Year and Date: 2015-12-03
  • Int'l Joint Research / Invited
• [Presentation] Role of the linker between oligomerization and DNA-binding domains of H-NS in gene silencing (2015)
  • Author(s): Yamanaka, Y., Winardhi, R.S., Yan, J., Kenney, L.J., Ishihama, A. and Yamamoto. K.
  • Organizer: Asian Conference in Transcription 2015
  • Place of Presentation: National University Sigapore, Singapore
  • Year and Date: 2015-12-03
  • Int'l Joint Research
• [Presentation] Cross-talk in transcriptional regulation between response regulators of Escherichia coli two- component system (2015)
  • Author(s): Yoshida, M., Yamamoto, K. and Ishihama, A
  • Organizer: Asian Conference in Transcription 2015
  • Place of Presentation: National University Sigapore, Singapore
  • Year and Date: 2015-12-03
  • Int'l Joint Research
• [Presentation] 大腸菌バイオフィルム形成統括因子CsgDの新規転写制御因子の同定と機能解明 (2015)
  • Author(s): 石塚俊行、石浜 明、小笠原 寛
  • Organizer: 第38回日本分子生物学会・第88回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫県神戸市
  • Year and Date: 2015-12-01
• [Presentation] 大腸菌べん毛形成マスターレギュレーターFlhDCの新規転写調節因子の同定と機能解明 (2015)
  • Author(s): 増井祥平、石塚俊行、Parul Singh、Aswin Sai Narain Seshasayee、石浜 明、小笠原 寛
  • Organizer: 第38回日本分子生物学会・第88回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫県神戸市
  • Year and Date: 2015-12-01
• [Presentation] 大腸菌二成分制御系EnvZ-OmpRの宿主内持続感染と宿主傷害性における役割 (2015)
  • Author(s): 白土明子，伊藤貴弘，黒田阿友美，島本尚人，山本兼由、石浜明、中西義信
  • Organizer: 第38回日本分子生物学会・第88回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫県神戸市
  • Year and Date: 2015-12-01
• [Presentation] 細菌ゲノムの段階的高次構造形成 (2015)
  • Author(s): 山中 幸、Yan Jie、Linda J Kenney、石浜 明、山本兼由
  • Organizer: 日本農芸化学会2015関東支部大会
  • Place of Presentation: お茶の水女子大学、東京都文京区
  • Year and Date: 2015-09-26
• [Presentation] 大腸菌二成分制御系EnvZ-OmpRによる宿主感染時の病原性の調節 (2015)
  • Author(s): 伊藤貴弘、黒田阿友美、山本兼由、石浜明、中西義信、白土明子
  • Organizer: 日本生化学会北陸支部第33回支部大会
  • Place of Presentation: 富山大学、富山県富山市
  • Year and Date: 2015-05-23
• [Presentation] 大腸菌シグマ因子RpoSによるショウジョウバエへの持続感染 (2015)
  • Author(s): 島本尚人、新田真緒、山本兼由、石浜明、中西義信、白土明子
  • Organizer: 日本生化学会北陸支部第33回支部大会
  • Place of Presentation: 富山大学、富山県富山市
  • Year and Date: 2015-05-23
• [Presentation] 筋萎縮性側索硬化症の封入体成分TDP-43タンパク質はRNA立体構造を認識する (2014)
  • Author(s): 石黒 亮、渡辺すみ子、渡邊裕斗、山本兼由、石浜 明
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (横浜、神奈川)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 異なる環境シグナルを認識するふたつの二成分制御系YedV/YedW, CusS/CusRによる共通標的遺伝子発現制御機構の解明 (2014)
  • Author(s): 浦野浩行、石浜 明、小笠原 寛
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (横浜、神奈川)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] CRP (cyclic AMP recepter protein)によるバイオフィルム形成調節因子MlrAの発現制御機構の解明 (2014)
  • Author(s): 小笠原 寛、小口卓也、佐野晃太郎、島田友裕、石浜 明
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (横浜、神奈川)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 大腸菌レスポンスレギュレーターFimZによる細胞形態制御 (2014)
  • Author(s): 小川綾乃、石浜 明、山本兼由
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (横浜、神奈川)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 細菌ヒストン様タンパク質H-NSによるゲノム転写制御の機能解析 (2014)
  • Author(s): 山本兼由、山中 幸、大島 拓、石浜 明
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (横浜、神奈川）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 100Sリボゾーム形成に関与するストレス応答因子群の探索 (2014)
  • Author(s): 吉田秀司、島田友裕、牧 泰史、古池 晶、上田雅美、和田千恵子、和田 明、石浜 明
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (横浜、神奈川）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Systematic search for stress-response factors influencing the 100S ribosome formation (2014)
  • Author(s): H. Yoshida, T. Shimada, Y. Maki, S. Furuike, M. Ueta, C. Wada, A. Wada, and A. Ishihama
  • Organizer: 2014 FEBS-EMBO
  • Place of Presentation: Palais des Congres de Paris (Paris, France)
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] 100Sリボソーム形成に影響を与えるストレス応答因子群の探索 (2014)
  • Author(s): 吉田秀司、島田友裕、牧 泰史、古池 晶、上田雅美、和田千恵子、和田 明、石浜 明
  • Organizer: 第16回 日本RNA学会
  • Place of Presentation: ウインク愛知 (名古屋、愛知)
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] 大腸菌GadEによる核様体抑制プロモーターの活性化 (2014)
  • Author(s): 山中 幸、志波 優、山本健太郎、川岸郁朗、吉川博文、石浜 明、山本兼由
  • Organizer: 第11回21世紀大腸菌研究会
  • Place of Presentation: ホテル大観 (盛岡、岩手)
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] 大腸菌バイオフィルム形成統括制御因子CsgDの新規発現調節機構の探索と機能解析 (2014)
  • Author(s): 小笠原寛、石塚俊行、石浜 明
  • Organizer: 第11回21世紀大腸菌研究会
  • Place of Presentation: ホテル大観 (盛岡、岩手)
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] 大腸菌RNAポリメラーゼRpoDホロ酵素によって認識されるConstitutive Promotersの探索と解析 (2014)
  • Author(s): 島田友裕、山崎由紀子、田中 寛、石浜 明
  • Organizer: 第11回21世紀大腸菌研究会
  • Place of Presentation: ホテル大観 (盛岡、岩手)
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] Prokaryotic genome regulation: Mutli-target regulators and multi-factor promoters (2014)
  • Author(s): Ishihama, A.
  • Organizer: 13th Asian Conference on Transcription
  • Place of Presentation: Univ. College, Melbourne, Australia
• [Presentation] Prokaryotic genome regulation: A revolutionary paradigm (2014)
  • Author(s): Ishihama, A.
  • Organizer: 17th Transcription Assembly
  • Place of Presentation: JNCASR, Bangalore, India
  • Invited
• [Presentation] 大腸菌GadEによるゲノム発現制御メカニズムの解明 (2014)
  • Author(s): 山中幸、志波優、川岸郁朗、吉川博文、石浜明、山本兼由
  • Organizer: 日本農芸化学会2014東京大会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] ビフィズス菌との細菌間相互作用に応答する大腸菌遺伝子発現機構 (2014)
  • Author(s): 小島渓晃、森田英利、石浜明、山本兼由
  • Organizer: 日本農芸化学会2014東京大会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] The whole set of constitutive promoters recognized by RNA polymerase RpoD holoenzyme of Escherichia coli (2014)
  • Author(s): Shimada, T., Yamazaki, Y., Tanaka, K. and Ishihama, A
  • Organizer: 法政大学マイクロナノテクノロジー研究開発センター公開シンポジウム
  • Place of Presentation: 法政大学マルチメディアホール
• [Presentation] Screening in vitro of promoter-specific transcription factors: Identification of TFs involved in regulation of the E. coli sdiA gene for control of cell division and differentiation (2014)
  • Author(s): Ishihama, A., Shimada, K., Ogasawara, Y., Shimada, T., Yamada, K., Kori, A., Yamamoto, K. and Kawagishi, I
  • Organizer: 法政大学マイクロナノテクノロジー研究開発センター公開シンポジウム
  • Place of Presentation: 法政大学マルチメディアホール
• [Presentation] 細菌の環境応答機構における制御ネットワーク (2014)
  • Author(s): 吉多美祐、安達友美、渡邊宏樹、石浜 明、山本 兼由
  • Organizer: 法政大学マイクロナノテクノロジー研究開発センター公開シンポジウム
  • Place of Presentation: 法政大学マルチメディアホール
• [Presentation] Transcription regulation by cAMP-CRP of the rmf gene for 10S ribosome formation (2013)
  • Author(s): Yoshida, H., Shimada, T., Maki, Y., Furuike, S., Uea, M., Wada, C., Wada, A. and Ishihama, A.
  • Organizer: The 18th Annu. Meet. RNA Society
  • Place of Presentation: Dovos, Switzerland
• [Presentation] 大腸菌における全tRNA遺伝子オペロンの発現プロファイルの解析-Fisの関与 (2013)
  • Author(s): 秋元隼風、長谷川喬彬、石浜 明、前田理久
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] ひとつのプロモーターを制御するすべての転写因子のPS-TF法による探索: 大腸菌細胞分裂分化制御因子SdiA遺伝子プロモーター (2013)
  • Author(s): 石浜 明、島田佳織、小笠原 寛、島田友裕、山田佳代子, 郡 彩子、山本兼由、川岸郁朗
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 大腸菌RNAポリメラーゼRpoDホロ酵素によって認識されるConstitutive Promotersの探索と解析 (2013)
  • Author(s): 島田友裕, 山崎由紀子, 田中 寛, 石浜 明
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 大腸菌の細胞形態形成制御に関する転写因子群の探索 (2013)
  • Author(s): 小川 綾乃、吉多美祐、石浜 明、山本 兼由
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 大腸菌RNAポリメラーゼに直接結合するタンパク質の網羅的解析 (2013)
  • Author(s): 平 優季、片山 映、石浜 明、山本兼由
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 大腸菌の二成分制御系レスポンスレギュレーター間における転写制御ネットワーク (2013)
  • Author(s): 吉多 美祐、安達友美、渡邊宏樹、石浜 明、山本 兼由
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 大腸菌核様体形成の主要蛋白H-NSのシステマティックな制御機能解析 (2013)
  • Author(s): 山本兼由、山中 幸、大島 拓、山田佳代子、岩田紀子、大村悦子、崎井裕貴、中川日出子、曽和義幸、川岸郁朗、石浜 明
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] PS-TF screeningによる大腸菌バイオフィルム統括制御因子csgDプロモーター特異的転写因子の探索 (2013)
  • Author(s): 小笠原 寛、石塚俊行、石浜 明
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 細菌細胞アミロイド様線維蛋白高発現に応答する転写二成分制御系 (2013)
  • Author(s): 加藤佑輝、石浜 明、小笠原 寛
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 大腸菌機能未知二成分制御系YedV/YedWによる遺伝子発現制御機構の解明 (2013)
  • Author(s): 浦野浩行、石浜明、小笠原 寛
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] 100Sリボゾームの形成に関係するrmf遺伝子の転写制御とストレス応答因子群の探索 (2013)
  • Author(s): 吉田秀司、島田友裕、牧 泰史、古池 晶、上田雅美、和田千恵子、和田 明、石浜 明
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア、国際会議場
• [Presentation] cAMP-CRPによる100Sリボソーム形成遺伝子rmfの転写制御 (2013)
  • Author(s): 島田友裕、吉田秀司、石浜 明
  • Organizer: 第7回日本ゲノム微生物学会若手の会研究会
  • Place of Presentation: ろうきん研修所富士センター
• [Presentation] 大腸菌コロニー形成に関与する新奇遺伝子morA (2013)
  • Author(s): 竹内真実、倉田竜明、石浜明、山本兼由
  • Organizer: 第12回微生物研究会
  • Place of Presentation: 東京電機大学
• [Presentation] Transcriptional regulatory cascade for response to acidic and anaerobic stresses in Escherichia coli (2013)
  • Author(s): 山中幸、大島拓、石浜明、山本兼由
  • Organizer: 第12回微生物研究会
  • Place of Presentation: 東京電機大学
• [Presentation] 大腸菌K12株の全二成分制御系遺伝子プロモーターの包括的発現解析 (2013)
  • Author(s): 渡邊宏樹、石浜明、山本兼由
  • Organizer: 第12回微生物研究会ビフィズス菌との細菌間相互作用に応答する大腸菌遺伝子発現機構
  • Place of Presentation: 東京電機大学
• [Book] Stress and Environmental Control of Gene Expression in Bacteria (2016)
  • Author(s): Ishihama, A.
  • Publisher: John Wiley & Sons
• [Book] Wiley and Blackwell Publishers (2015)
  • Author(s): A. Ishihama
  • Publisher: Stress Response and Environmental Control of Gene Expression in Bacteria
• [Remarks] TECデータベース