| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
The genome of the model prokaryote Escherichia coli contains approximately 4,500 genes. Expression pattern of the genome is determined through selective transcription by the RNA polymerase. Its gene selectivity is controlled at two steps through interaction with seven sigma factors and about 300 transcription factors. For identification of regulatory targets by transcription regulators, we identified their binding sites on the E. coli genome using Genomic SELEX system. We have so far identified the regulatory targets for all seven sigma factors and for more then 250 transcription factors. The results altogether are assembled into a single database TEC (Transcription Profile of Escherichia coli) (www.shigen.nig.ac.jp/ecoli/tec/), which is now open to public through NIG (National Institute of Genetics). Noteworthy is that this is the first success of identification of the regulatory roles of the whole set of transcription regulators form a single organism.
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