| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
We collected family members developed lung cancer, gastric cancer or sarcoma, and performed whole-exome sequencing on DNA from their blood. A CHEK2 mutation detected in lung cancer siblings was concluded to be the cause of multiple primary lung cancer with accompanying cancers in other organs. In other cases, mutations that caused cancers were not detected.
We developed a high sensitive method to detect rare mutations in blood from cancer patients using deep sequencing. Further, by using molecular barcode technology, we developed a method to determine the nucleotide sequence with high accuracy, and to absolutely quantify DNA molecules.
|
